Single Nucleotide Polymorphisms in a Gene for Translation Initiation Factor (eIF4G) of Rice (Oryza sativa) Associated with Resistance to Rice tungro spherical virus

Abstract

Rice tungro disease (RTD) is a serious constraint to rice production in South and Southeast Asia. RTD is caused by Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Rice cv. Utri Merah is resistant to RTSV. To identify the gene or genes involved in RTSV resistance, the association of genotypic and phenotypic variations for RTSV resistance was examined in backcross populations derived from Utri Merah and rice germplasm with known RTSV resistance. Genetic analysis revealed that resistance to RTSV in Utri Merah was controlled by a single recessive gene (tsv1) mapped within an approximately 200-kb region between 22.05 and 22.25 Mb of chromosome 7. A gene for putative translation initiation factor 4G (eIF4G(tsv1)) was found in the tsv1 region. Comparison of eIF4G(tsv1) gene sequences among susceptible and resistant plants suggested the association of RTSV resistance with one of the single nucleotide polymorphism (SNP) sites found in exon 9 of the gene. Examination of the SNP site in the eIF4G(tsv1) gene among various rice plants resistant and susceptible to RTSV corroborated the association of SNP or deletions in codons for Val(1060-1061) of the predicted eIF4G(tsv1) with RTSV resistance in rice.

Retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV en México. II. Oportunidades para la selección asistida

“…En efecto, a la fecha todos los genes recesivos involucrados en las interacciones planta-potyvirus, grupo al que pertenecen BCMV y BCMNV, se han caracterizado como factores del inicio de la traducción, principalmente eIF4E, eIF4G o sus isoformas (Wang et al, 2011). Estos incluyen 14 genes de resistencia recesivos, de los cuales 12 son eIF4E o su isoforma y dos eIF4G o su isoforma (Albar et al, 2006;Lee et al, 2010). Por lo tanto, la familia de proteínas eIF4E y eIF4G son particularmente importantes, pues son esenciales tanto para la susceptibilidad como la resistencia a las infecciones por algunos virus de ARN (Potyvirus, Cucumovirus, Carmovirus y Bymovirus) en plantas como Arabidopsis, Capsicum spp., Lactuca spp., Lycopersicon spp., Cucumis melo, y Hordeum vulgare (Robaglia y Caranta, 2006).…”

“…Indeed, to date all recessive genes involved in plant-potyvirus interactions, group to which BCMV and BCMNV belong, it have been characterized as factors in the initiation of translation, mainly eIF4E, eIF4G or its isoforms (Wang et al, 2011). These include 14 recessive resistance genes, of which 12 are eIF4E or its isoform and two eIF4G or its soform (Albar et al, 2006;Lee et al, 2010). Therefore, the protein family eIF4E and eIF4G are particularly important because they are essential for both susceptibility and resistance to infection by some RNA virus (Potyvirus, Cucumovirus, Carmovirus and bymovirus) in plants such as Arabidopsis, Capsicum spp., Lactuca spp., Lycopersicon spp., Cucumis melo, and Hordeum vulgare (Robaglia and Caranta, 2006).…”

Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana

“…Overexpression of eIF1A improved tolerance to salt stress (Rausell et al, 2003), while overexpression of eIF5A increased resistance to osmotic, nutrient, oxidative, and high temperature stresses (Ma et al, 2010;Xu et al, 2011;Wang et al, 2012). eIF4G plays significant roles against tungro spherical virus ( Lee et al, 2010), and eIF4E is involved in resistance against plum pox virus (Wang et al, 2013), potato virus Y (Duan et al, 2012), and maize rough dwarf disease (Shi et al, 2013). Thus, specific manipulation of initiation factors may afford an approach to improve plant survival under stress or pathogen attack.…”

Application and utilization of marker assisted selection for biotic stress resistance in hybrid rice (Oryza sativaL.)

“…Rice blast disease caused by the ascomycete fungus, Magnaporthe oryzae (Couch and Kohn 2002), is also one of the leading causes of great yield loss of rice worldwide and is the most devastating fungal disease on cultivated rice as well as other species of the Poaceae (Zeigler et al 1994;Talbot and Foster 2001;Talbot 2003;Niño et al 2015). Rice tungro disease (RTD) is caused by two viruses, Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus (RTBV), and is one of the most serious diseases of rice in South and Southeast Asia (Lee et al 2010). Rice damaged by RTD show symptoms, such as small growth and yellow-orange discoloration of leaves (Hibino 1983).…”