Single Nucleotide Polymorphisms from CSF2, FLT1, TFPI and TLR9 Genes Are Associated with Prelabor Rupture of Membranes

Abstract

A prelabor rupture of membranes (PROM) and its subtypes, preterm PROM (pPROM) and term PROM (tPROM), are associated with disturbances in the hemostatic system and angiogenesis. This study was designed to demonstrate the role of single nucleotide polymorphisms (SNPs), localized in CSF2 (rs25881), FLT1 (rs722503), TFPI (C-399T) and TLR9 (rs352140) genes, in PROM. A population of 360 women with singleton pregnancy consisted of 180 PROM cases and 180 healthy controls. A single-SNP analysis showed a similar distribution of genotypes in the studied polymorphisms between the PROM or the pPROM women and the healthy controls. Double-SNP TT variants for CSF2 and FLT1 polymorphisms, CC variants for TLR9 and TFPI SNPs, TTC for CSF2, FLT1 and TLR9 polymorphisms, TTT for FLT1, TLR9 and TFPI SNPs and CCCC and TTTC complex variants for all tested SNPs correlated with an increased risk of PROM after adjusting for APTT, PLT parameters and/or pregnancy disorders. The TCT variants for the CSF2, FLT1 and TLR9 SNPs and the CCTC for the CSF2, FLT1, TLR9 and TFPI polymorphisms correlated with a reduced risk of PROM when corrected by PLT and APTT, respectively. We concluded that the polymorphisms of genes, involved in hemostasis and angiogenesis, contributed to PROM.

Effects of chronic hypoxia on the gene expression profile in the embryonic heart in three Chinese indigenous chicken breeds (Gallus gallus)

“…ESCO2 was upregulated during fin regeneration and specifically within the blastema. ESCO2 knockdown significantly reduced the CX43/GJA1 expression, which was required for cell-cell communication (22).…”

Association of Single Nucleotide Polymorphisms from Angiogenesis-Related Genes, ANGPT2, TLR2 and TLR9, with Spontaneous Preterm Labor

“…We have ruled out inter alia, women with pPROM or cervical insufficiency both from the PTL and control cohorts studied, all of which can be considered to be the strengths of our research. Among the various risk factors of PTL, pPROM is one of the most serious pregnancy complications, causing one-third of all PTLs and found in approximately 3-4% of all deliveries [101]. Cervical insufficiency occurs in 0.05 to 2.0% of pregnant women and is a well-known risk factor for PTD and mid-trimester pregnancy loss [102].…”

Associations of Maternal Serum Iodine Concentration with Obstetric Complications and Birth Outcomes—Longitudinal Analysis Based on the Huizhou Mother–Infant Cohort, South China

“…Our analysis found an increased risk of PROM in the highest quartile of SIC during early pregnancy, and the association was more evident after the exclusion of women with preterm delivery. Although we adjusted the range of possible confounders in the multivariable logistic regression model, residual confounding was unavoidable as we did not investigate infection or trauma before or at delivery, the diseased condition of the membrane itself, and the polymorphisms of genes involved in hemostasis and angiogenesis contributing to PROM [28]. Although there has been no report on SIC and PROM, studies on maternal thyroid dysfunction and the risk of PROM have reported inconsistent findings [29,30].…”