Single nucleotide polymorphism rs4284505 in microRNA17 and risk of dental fluorosis

Abstract

Objectives: The aim of this study is to evaluate the association between the single nucleotide polymorphism (SNP) rs4284505 within the gene that codifies microRNA17 (miRNA17) and dental fluorosis (DF) in a group of children. Methods: Children living in a city with fluoridation of public water supplies were included. DF was assessed in erupted permanent teeth by Dean's modified index. The miR-SNP rs4284505 was selected in miRNA17 and genotyping was carried out by real-time PCR. Genotype and allelic distributions between DF and control, and between DF phenotypes (mild, moderate and severe) and control were analysed. Results: Among a total of 527 children enrolled for the study, 383 were DF free and 144 presented DF. In the dominant model analysis (AA þ AG vs. GG) the miR-SNP rs4284505 was associated with moderate DF, with carriers of the GG genotype having an increased risk of more than two times for DF (p ¼ 0.031; Odds Ratio ¼ 2.26, Confidence Interval 95%¼ 1.04-4.73). Allelic distribution showed borderline statistical significance for moderate DF with the carriers of G allele having an increased risk for DF (p ¼ .050; Odds Ratio ¼ 1.75, Confidence Interval 95%¼ 1.00-3.12). Conclusion: The miR-SNP rs4284505 in miRNA17 was associated with an increased risk of DF.

Single Nucleotide Polymorphisms and Dental Fluorosis: A Systematic Review

“…These teams analyzed TUFT1 rs4970957 and miRNA17 rs4284505, indicating that alleles A and G increased the risk of dental fluorosis. In addition, a second team reported that the association occurred with the moderate phenotype of the disease [ 24 , 32 ].…”

“…D4: Bias in measurement of the outcome. D5: Bias in the selection of the report result [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ].…”

The genetic determinants of oral diseases in Africa: The gaps should be filled

“…and is associated with increased dental caries ( 54 , 55 ). Dental fluorosis presents with differing severity in individuals exposed to similar levels of fluoride intake, suggesting the influence of genetic factors and gene-environment interactions ( 56 ).…”

Do methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 polymorphisms modify changes in intelligence of school-age children in areas of endemic fluorosis?

“…[18,19] Several studies have reported that genetic polymorphisms can modify the harmful effects of fluoride, such as dental and skeletal fluorosis. [20,21] Studies have also shown that gene polymorphisms may modulate sensitivity to the effects of fluoride exposure on intelligence. For example, polymorphisms of the catechole-o-methyltransferase gene and dopamine receptor-2 gene have been reported to modify the adverse effects of fluoride on IQ scores.…”

“…Individual susceptibility (eg, gene polymorphisms) is an important factor that affects the sensitivity of body health to environmental factors [18,19] . Several studies have reported that genetic polymorphisms can modify the harmful effects of fluoride, such as dental and skeletal fluorosis [20,21] . Studies have also shown that gene polymorphisms may modulate sensitivity to the effects of fluoride exposure on intelligence.…”