Single nucleotide polymorphism genotyping of ALDH2 gene based on asymmetric PCR and fluorescent probe-mediated melting curves

Zhang, Limei; Liú, Dan; Li, Baolin; Xie, Jingling; Liu, Jinbo; Zhang, Zhang

doi:10.1016/j.ab.2021.114509

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Recently we developed an endpoint genotyping assay using two TaqMan probes which are a FAM-labeled probe for detection of the se del2 allele and a VIC-labeled probe for detection of the FUT2 coding region [12]. The TaqMan probes are also available for fluorescence melting curve analysis (FMCA) to detect SNPs [18][19][20][21]. FMCA is one of the most robust SNP detection methods [18].…”

Section: Introductionmentioning

confidence: 99%

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Soejima¹,

Koda²

2023

Preprint

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The α(1,2)fucosyltransferase (Se enzyme) encoded by FUT2 is involved in the secretor status of ABH(O) blood group antigens. The sedel2 allele is one of the non-functional FUT2 (se) alleles in which 9.3 kb containing the entire coding region of FUT2 is deleted by Alu-mediated nonhomologous recombination. In addition to this allele, three SNPs of FUT2, c.375A>G, c.385A>T, and c.571C>T, appear to be prevalent in certain Oceanian populations such as Polynesians. Recently, we developed an endpoint genotyping assay to determine the sedel2 zygosity, using a FAM-labeled probe for detection of the sedel2 allele and a VIC-labeled probe for detection of FUT2. In this study, instead of the VIC probe, a HEX-labeled probe covering both c.375A>G and c.385A>T and a Cy5-labeled probe covering c.571C>T were added to the sedel2 allele assay mixture to allow simultaneous detection of these four variations by endpoint genotyping for the sedel2 zygosity and fluorescence melting curve analysis for c.375A>G, c.385A>T, and c.571C>T genotyping. The results obtained from 24 Samoan subjects with this method were identical to those obtained with previous methods. Therefore, it appears that present method can accurately determine these four variations simultaneously.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Soejima¹,

Koda²

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Recently, we developed an endpoint genotyping assay using two TaqMan probes, which were a FAM-labeled probe for detection of the se del2 allele and a VIC-labeled probe for detection of the FUT2 coding region [ 12 ]. The TaqMan probes are also available for fluorescence melting curve analysis (FMCA) to detect SNPs [ 18 , 19 , 20 , 21 ]. FMCA is one of the most robust SNP detection methods [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of c.375A>G, c.385A>T, c.571C>T, and sedel2 of FUT2 via Real-Time PCR in a Single Tube

Soejima

Koda

2023

Diagnostics

View full text Add to dashboard Cite

α(1,2)fucosyltransferase (Se enzyme) encoded by FUT2 is involved in the secretor status of ABH(O) blood group antigens. The sedel2 allele is one of the non-functional FUT2 (se) alleles in which 9.3 kb, containing the entire coding region of FUT2, is deleted by Alu-mediated nonhomologous recombination. In addition to this allele, three SNPs of FUT2, c.375A>G, c.385A>T, and c.571C>T, appear to be prevalent in certain Oceanian populations such as Polynesians. Recently, we developed an endpoint genotyping assay to determine sedel2 zygosity, using a FAM-labeled probe for detection of the sedel2 allele and a VIC-labeled probe for the detection of FUT2. In this study, instead of the VIC probe, a HEX-labeled probe covering both c.375A>G and c.385A>T and a Cy5-labeled probe covering c.571C>T were added to the sedel2 allele assay mixture to allow for the simultaneous detection of these four variations via endpoint genotyping for sedel2 zygosity and fluorescence melting curve analysis for c.375A>G, c.385A>T, and c.571C>T genotyping. The results obtained from 24 Samoan subjects using this method were identical to those obtained using previous methods. Therefore, it appears that the present method can accurately determine these four variations simultaneously.

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Single nucleotide polymorphism-based biomarker in primary hypertension

Laxmi,

Golmei,

Srivastava

et al. 2024

European Journal of Pharmacology

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Single nucleotide polymorphism genotyping of ALDH2 gene based on asymmetric PCR and fluorescent probe-mediated melting curves

Cited by 3 publications

References 29 publications

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Detection of c.375A>G, c.385A>T, c.571C>T, and sedel2 of FUT2 via Real-Time PCR in a Single Tube

Single nucleotide polymorphism-based biomarker in primary hypertension

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