Single nucleotide polymorphism association study for backfat and intramuscular fat content in the region between SW2098 and SW1881 on pig chromosome 61

Lee, K. T.; Byun, Mi Jeong; Kang, Ki Ser; Hwang, Hye‐Yeon; Park, E. W.; Kim, J. M.; Kim, T. H.; Lee, S. H.

doi:10.2527/jas.2011-4228

Cited by 7 publications

(8 citation statements)

References 40 publications

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“…In the present study, the c.919G>A, 307G>R CBG variant was significantly associated with backfat thickness, postmortem pH 24h , CIE a*, CIE b*, water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force (Table 2). Its association with drip loss has been also reported previously (Esteve et al 2011; Lee et al (2012), who reported that backfat thickness and fat content are associated with lean-meat content. Furthermore, in the present study, the variant G allele was associated with decreases in backfat thickness, post-mortem pH 24h , CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force.…”

Section: Discussionsupporting

confidence: 76%

Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs

et al. 2016

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Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G>A, which corresponds to 307G>R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH 24h , meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH 24h , CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meatquality traits.Key words: CBG, Berkshire, nsSNP, cortisol, meat-quality trait, molecular marker.Résumé : La transcortine (CBG, « corticosteroid-binding globulin ») est une protéine de transport plasmatique qui a une activité de liaison aux glucocorticoïdes. Dans la présente étude, nous avons identifié l'expression du gène CBG dans plusieurs tissus de quatre races de porcs : Berkshire, Duroc, Landrace et Yorkshire. L'expression de l'ARNm de CBG a été détectée dans le foie des quatre races et était la plus élevée chez les porcs Berkshire. Nous avons aussi trouvé des polymorphismes mononucléotidiques (SNP, « single nucleotide polymorphism ») dans le gène CBG chez les porcs Berkshire, incluant SNP c.919G>A, qui correspond à 307G>R. Nous avons analysé les relations entre cette variante CBG et diverses caractéristiques de qualité de viande. Le SNP était associé de façon significativement à l'épaisseur du gras dorsal, le pH post-mortem 24 h, la couleur de la viande [CIE a* (rougeur), CIE b* (degré de couleur jaune)], la capacité de rétention d'eau, la teneur en gras, la teneur en humidité, la teneur en protéines, les pertes à la cuisson et les forces de cisaillement. Par contre, les effets différaient selon le genre : les valeurs étaient associées de façon significative avec presque toutes les caractéristiques chez les truies nullipares, tandis l'association n'était significative que pour les pertes à la cuisson et les forces de cisaillement chez les castrats. L'allèle variant G était associé à une diminution d'épaisseur de gras dorsal, de pH post-mortem 24 h, de CIE a*, de teneur en gra...

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Section: Discussionsupporting

confidence: 76%

Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs

et al. 2016

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“…Of the four polymorphic sites, g.955T>C SNP was previously found (kps6413; Lee et al, 2012), and the other SNPs were newly identified in this study (g.196A>G; ss974514574, g.392C>A; ss974514575, g.3247A>T; ss974514576). To estimate the genotypic and allelic frequencies for the four SNPs, 678 Berkshire pigs were genotyped and the results are given in Table 2.…”

Section: Snp Identification and Association Studysupporting

confidence: 57%

“…It was also associated with drip loss, MC_L, and moisture traits (P < 0.05). GADD45A was previously reported to be significantly associated with IMF and BF traits in a reference family crossed by Korean native pig and Landrace (Lee et al, 2012). Despite the different pig populations, this SNP had the same effect on IMF and BF traits.…”

Section: Snp Identification and Association Studymentioning

confidence: 86%

“…In pigs, the GADD45A gene is located on SSC6 (Kim et al, 2006). Previously, the porcine GADD45A was suggested to be a powerful marker of IMF and BF traits (Lee et al, 2012). However, the structural variation and regulatory functions of porcine GADD45A have not been examined.…”

Section: Snp Identification and Association Studymentioning

confidence: 99%

“…Polymorphisms in LEPR and h-FABP have been reported to be associated with IMF (Mackowski et al, 2005;Ovilo et al, 2005;Gerbens et al, 1997Gerbens et al, , 1999Gerbens et al, , 2000. Recently, the growth arrest and DNA-damage-inducible protein 45 alpha gene (GADD45A) was reported to be a potential candidate gene controlling QTL for BF and IMF traits (Lee et al, 2012). It was reported that GADD45A, which is located near LEPR, mapped to the pig chromosome 6q32 region, associated with a QTL for fat-deposit traits (Kim et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Novel SNPs in the growth arrest and DNA damage-inducible protein 45 alpha gene (GADD45A) associated with meat quality traits in Berkshire pigs

Cho¹,

Lee²,

Choi³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study was conducted to evaluate the porcine gene GADD45A (growth arrest and DNA-damage-inducible protein 45 alpha) as a positional candidate controlling quantitative trait loci (QTL) for meat quality traits on chromosome 6 (SSC6). Four exons of the porcine GADD45A gene were defined from cDNA and BAC clone sequences. A total of 4 single nucleotide polymorphisms (SNPs) were identified in porcine GADD45A. The association of these SNPs (g.196A>G, g.392C>A, g.955T>C and g.3247A>T) with meat quality traits was evaluated in 678 Berkshire pigs. The genotype distribution of only one SNP (g.3247A>T) conformed to Hardy Weinberg equilibrium in the pig population analyzed in this study, and the other SNPs were not in Hardy-Weinberg equilibrium. All four SNPs were significantly associated with meat quality traits. Three SNPS (g.196A>G, g.392C>A, (2015) and g.955T>C) showed similar significant association patterns for drip loss, cooking loss, meat color (lightness; MC_L and yellowness; MC_B), shear force and water-holding capacity traits. By contrast, g.3247A>T had a different association pattern with other traits such as intramuscular fat content (IMF) and backfat thickness (BF), drip loss, MC_L, and moisture. These findings will provide useful information for genetic characterization or association studies in other pig populations. Additionally, these markers can potentially be applied in pig breeding programs to improve meat quality traits, including IMF and BF.

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Genome-wide association studies for seven production traits highlight genomic regions useful to dissect dry-cured ham quality and production traits in Duroc heavy pigs

Bertolini

Schiavo

Galimberti

et al. 2018

Animal

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Protected designation of origin dry-cured hams are obtained from heavy pigs (slaughtered at about 160 kg of live weight). A specific breeding program designed to improve meat quality for this production has included as key traits the level of intermuscular fat between the leg muscles and ham weight loss during the seasoning period together with a balance between fat and lean cuts. In this study we carried out genome-wide association studies for seven traits used in the genetic merit of Italian Duroc heavy pigs, five related to meat and carcass quality traits (visible intermuscular fat, ham weight loss at first salting, backfat thickness, ham weight and lean cuts), and two related to performance and efficiency traits (average daily gain and feed : gain ratio). A total of 573 performance-tested pigs were genotyped with the Illumina PorcineSNP60 BeadChip and genome-wide association analyses were carried out using the Bayes B approach with the 1 Mb window option of GenSel and random residuals for each of the seven traits. Detected windows were supported by independent single nucleotide polymorphism analyses with a linear mixed model (LMM) approach on the same animals for the same traits. A total of 30 windows identifying different quantitative trait loci (QTL) were detected and among those, 27 were confirmed by LMM in one of these traits. Among the confirmed windows, three QTL were reported for visible intermuscular fat, seven for ham weight loss at first salting and five and four for backfat thickness and lean cut, respectively. A total of eight QTL were detected for the other production traits. No overlapping QTL were reported except for one window on porcine chromosome 10 between lean cuts and ham weight that contained the CACNB2 gene that has been already associated with loin marbling score in other Duroc pigs. Several regions contained genes that have been already associated with production traits in other pig breeds, including Duroc lines, related to fat deposition or muscle structure. This work reports, for the first time, genome-wide association study results for several traits in Italian Duroc heavy pigs. These results will be useful to dissect the genetic basis for dry-cured ham production traits that determine the total genetic merit index of Italian Duroc pigs.

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Single nucleotide polymorphism association study for backfat and intramuscular fat content in the region between SW2098 and SW1881 on pig chromosome 61

Cited by 7 publications

References 40 publications

Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs

Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs

Novel SNPs in the growth arrest and DNA damage-inducible protein 45 alpha gene (GADD45A) associated with meat quality traits in Berkshire pigs

Genome-wide association studies for seven production traits highlight genomic regions useful to dissect dry-cured ham quality and production traits in Duroc heavy pigs

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