Single nucleotide polymorphism associated with disease resistance in Penaeus vannamei

Mangabeira-Silva, Iasmim Santos; Soares, Paulo Eduardo; Lanza, Daniel Carlos Ferreira

doi:10.1016/j.jip.2020.107498

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…Hundreds of SNPs related to resistance have been identified, and a number of targeted proteins have been proposed for L . vannamei [ 18 , 21 ]. As expected, many innate immune genes, including those encoding anti-lipopolysaccharide factor, ubiquitin carboxyl-terminal hydrolase, PI3K regulatory subunit, Toll-like receptors, and hemocyanin, have been proposed to be targets for resistance in association studies [ 18 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…vannamei [ 18 , 21 ]. As expected, many innate immune genes, including those encoding anti-lipopolysaccharide factor, ubiquitin carboxyl-terminal hydrolase, PI3K regulatory subunit, Toll-like receptors, and hemocyanin, have been proposed to be targets for resistance in association studies [ 18 , 21 ]. Whereas, it remains unclear whether these genes play a key role in resistance, and no relationship has been found between them and colonization resistance.…”

Section: Discussionmentioning

confidence: 99%

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Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

Yuan,

Yu,

et al. 2024

PLoS Pathog

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Vibriosis is one of the most serious diseases that commonly occurs in aquatic animals, thus, shaping a steady inherited resistance trait in organisms has received the highest priority in aquaculture. Whereas, the mechanisms underlying the development of such a resistance trait are mostly elusive. In this study, we constructed vibriosis-resistant and susceptible families of the Pacific white shrimp Litopenaeus vannamei after four generations of artificial selection. Microbiome sequencing indicated that shrimp can successfully develop a colonization resistance trait against Vibrio infections. This trait was characterized by a microbial community structure with specific enrichment of a single probiotic species (namely Shewanella algae), and notably, its formation was inheritable and might be memorized by host epigenetic remodeling. Regardless of the infection status, a group of genes was specifically activated in the resistant family through disruption of complete methylation. Specifically, hypo-methylation and hyper-expression of genes related to lactate dehydrogenase (LDH) and iron homeostasis might provide rich sources of specific carbon (lactate) and ions for the colonization of S. algae, which directly results in the reduction of Vibrio load in shrimp. Lactate feeding increased the survival of shrimp, while knockdown of LDH gene decreased the survival when shrimp was infected by Vibrio pathogens. In addition, treatment of shrimp with the methyltransferase inhibitor 5-azacytidine resulted in upregulations of LDH and some protein processing genes, significant enrichment of S. algae, and simultaneous reduction of Vibrio in shrimp. Our results suggest that the colonization resistance can be memorized as epigenetic information by the host, which has played a pivotal role in vibriosis resistance. The findings of this study will aid in disease control and the selection of superior lines of shrimp with high disease resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

Yuan,

Yu,

et al. 2024

PLoS Pathog

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“…The association of STR markers with specific phenotypes in marine shrimp Penaeus vannamei has been relatively unexplored, primarily due to the availability of new SNP-based genotyping methods [ 18 ]. Unlike SNPs, which typically require around 60 to 100 markers for genotyping individuals, a small number of STRs (sometimes even fewer) can accomplish the same task [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome

Mangabeira-Silva,

Soares,

Vieira da Silva

et al. 2024

PLoS ONE

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In this study, an extensive analysis of microsatellite markers (Single Tandem Repeats—STRs) in Penaeus vannamei was conducted at an advanced level. The markers were thoroughly examined, characterized, and specific markers located within coding regions were identified. Out of a total of 306 STRs, 117 were classified as perfect markers based on their single repeat motif. Among these perfect markers, 62 were found to be associated with predicted coding genes (mRNA), which were involved in various functions such as binding, catalytic activity, ATP-dependent activity, transcription, structural and molecular regulation. To validate the accuracy of the findings, a sample of nine markers was subjected to in vitro testing, which confirmed the presence of polymorphisms within the population. These results suggest the existence of different protein isoforms within the population, indicating the potential of these markers for application in both population and phenotype-genotype association studies. This innovative approach opens up new possibilities for investigating the impact of genomic plasticity in populations of P. vannamei.

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Transcriptomic analysis reveals nanoplastics-induced apoptosis, autophagy and immune response in Litopenaeus vannamei

Li,

Ye,

Zhu

et al. 2024

Science of The Total Environment

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Single nucleotide polymorphism associated with disease resistance in Penaeus vannamei

Cited by 3 publications

References 63 publications

Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome

Transcriptomic analysis reveals nanoplastics-induced apoptosis, autophagy and immune response in Litopenaeus vannamei

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