Single-molecule trapping and measurement in solution

Bespalova, Maria; Sushanta, Mahanta,; Krishnan, Madhavi

doi:10.1016/j.cbpa.2019.05.013

Cited by 22 publications

(16 citation statements)

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“…As stated earlier, DEP is a phenomenon where a polarizable particle can be moved when placed in a non-uniform electric field with an intensity gradient. DEP trapping offers fast, non-invasive, force-based manipulation of biomolecules [342,343]. Ghomian et al developed a low-cost DEP-based trapping platform that offers easy and reproducible fabrications of devices [341].…”

Section: Trapping Single-moleculesmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

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Section: Trapping Single-moleculesmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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“…A growing area of research explores non-invasive techniques based on spatial confinement or surface charge induced trapping of single-molecules to extend observation times. 16,17 Here, we address these challenges by confining single FRET-labelled molecules non-invasively in solution with electrokinetic trapping in an Anti-Brownian ELectrokinetic trap (ABEL trap). 18–20 The ABEL trap confines a molecule's Brownian motion by applying an electric force acting on its surface charge (Fig.…”

Section: Introductionmentioning

confidence: 99%

Single-molecule FRET combined with electrokinetic trapping reveals real-time enzyme kinetics of individual F-ATP synthases

Sielaff

Dienerowitz

2022

Nanoscale

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“…To address these limitations and observe proteins and molecular motors in action a growing field of research devoted to the development of single-molecule confinement tools gained momentum during the past decade [17][18][19]. The challenge is to monitor a single proteintypically 5-10 nm in effective diameter -long enough to detect conformational changes during one reaction cycle of its natural function while avoiding the need for surface immobilization.…”

Section: Introductionmentioning

confidence: 99%