Single‐Molecule Sensors: Challenges and Opportunities for Quantitative Analysis

Gooding, J. Justin; Gaus, Katharina

doi:10.1002/anie.201600495

Cited by 244 publications

(279 citation statements)

References 89 publications

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“…The second reason is that if quantitation is achieved via molecular counting, the sensors can be calibration free. Third, if one can monitor how single molecules interact with the sensor, then it may be possible to differentiate specific binding events from nonspecific binding events . The significant challenge with single molecule measurement relates to the background noise from a large number of other species within the sample.…”

Section: Introductionmentioning

confidence: 99%

How Nanoparticles Transform Single Molecule Measurements into Quantitative Sensors

Bennett

Tilley

et al. 2019

Advanced Materials

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.201904339.Single molecule measurements are revolutionizing the understanding of the stochastics of behavior of single molecules. There is a common theme referred to as a near-field approach, in how many single molecule measurements are being performed in assays. The term near field is used because the measurement volume is typically very small such that a single molecule, or a single molecule binding pair, within that volume is of an appreciable concentration. The next development in detection will be performing many single molecule measurements at one time such that single molecule measurements can be used as the basis for quantitative analysis. There have already been some notable developments in this direction. Again, all have a common theme in that nanoparticles are used to create many near-field volumes that can be measured simultaneously. Herein, the coupled developments in nanoparticles and measurement strategies that allow nanoparticles to be the backbone of the next generation of sensing technologies are discussed. 8 of 8) www.advmat.de www.advancedsciencenews.com (

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Section: Introductionmentioning

confidence: 99%

How Nanoparticles Transform Single Molecule Measurements into Quantitative Sensors

Bennett

Tilley

et al. 2019

Advanced Materials

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“…Universal label-free detection and characterization of single biomolecules, in particular proteins, is a grand ambition in the development of diagnostic sensors. [1] Beyond the obvious advantage that single-molecule biosensors feature ultimate sensitivity with detection at the fundamental limit of one single molecule, such sensors would be able to spot rare aberrant biomolecules in an abundant background of healthy ones, [2] probe substructure of single molecules, [3] and allow to study behavior of single-molecular interactions, [4] ideally all without the need for chemical labeling. Two important approaches that are being explored to achieve such sensors are plasmonic nanoantenna apertures [4] and nanopores, both biological [5] and solid-state nanopores.…”

Section: Introductionmentioning

confidence: 99%

Nano‐Optical Tweezing of Single Proteins in Plasmonic Nanopores

2019

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Single‐molecule sensing technologies aim to detect and characterize single biomolecules, but generally need labeling while the measurement times and throughput are severely restricted by a lack of positional control over the molecule. Here, a plasmonic nanopore biosensor is reported where single molecules can be electrophoretically delivered into a nanopore sensor with a plasmonic nanoantenna that is used to optically trap single molecules for extended measurement times. Using the light transmission through the antenna as read‐out, optical trapping of 20 nm diameter polystyrene nanoparticles and individual beta‐amylase proteins, a 200 kDa enzyme, in the plasmonic nanoantenna are demonstrated. Application of an electrical bias voltage allows the increase of the event rate over an order of magnitude as well as shorten the residence time of the proteins in the plasmonic nanopore as they can controllably be drawn out of the trap by electrical forces. Trapping is found to be assisted by protein–surface interactions and trapped proteins can denature on the nanopore surface. The integration of two single‐molecule sensors, a plasmonic nanoantenna and solid‐state nanopore, creates independent control handles at the single‐molecule level—the optical trapping force and electrophoretic force—which provides augmented control over single molecules.

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“…[1] Simple molecular electronic functional devices, such as molecular rectifiers, [2,3] molecular switches, [4,5] molecular wires [6,7] and molecular sensors [8][9][10] have been designed theoretically and synthesized experimentally on single-molecular scale. [1] Simple molecular electronic functional devices, such as molecular rectifiers, [2,3] molecular switches, [4,5] molecular wires [6,7] and molecular sensors [8][9][10] have been designed theoretically and synthesized experimentally on single-molecular scale.…”

Section: Introductionmentioning

confidence: 99%

Spin Logic Gates Operated by Protonation and Magnetism in Molecular Combinational Circuits

Zhao

Zou

Sun

et al. 2019

Advcd Theory and Sims

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The protonation and magnetism effects on the spin transport properties of benzo[b]phenazine (BPE) and bis(o-phenylenediamine) manganese (IV) complex (Mn-OPD) molecules in series and in parallel are theoretically investigated using density functional theory (DFT) combined with nonequilibrium Green's function method (NEGF). The spin-resolved currentvoltage curves show that the magnetic field mainly regulate the spin-polarized direction of current, and the protonation effect will significantly reduce the magnitude of spin-polarized current. Moreover, under two orthogonal inputs, the molecular combinational circuits can behave as AND and OR spin logic gates, suggesting the application potential of integrating logic operations for future spin-based nanoelectronics. In addition, we have observed the Fano resonance phenomenon in the parallel molecular junction, and the Fano resonance feature can be canceled by protonation effect. This work proposes a feasible way to construct a complex single-molecule spin logic gate device.

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Single‐Molecule Sensors: Challenges and Opportunities for Quantitative Analysis

Cited by 244 publications

References 89 publications

How Nanoparticles Transform Single Molecule Measurements into Quantitative Sensors

How Nanoparticles Transform Single Molecule Measurements into Quantitative Sensors

Nano‐Optical Tweezing of Single Proteins in Plasmonic Nanopores

Spin Logic Gates Operated by Protonation and Magnetism in Molecular Combinational Circuits

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