Single Molecule Protein Segments Sequencing by a Plasmonic Nanopore

Zhou, Juan; Lan, Qing; Li, Wang; Ji, Lina; Wang, Kang; Xia, Xing‐Hua

doi:10.1021/acs.nanolett.3c00086

Cited by 16 publications

(15 citation statements)

References 57 publications

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“…We found that the amino acid residues played an important role in the conformational change of CaM. Similar method was also utilized to study the unfolding and multistep sequential translocation of a single cytochrome c molecule through a SERS active gold plasmonic nanopore (Figure b) …”

Section: Advancements In Nanopore Sensorsmentioning

confidence: 94%

Developing Solid-State Single-, Arrayed-, and Composite-Nanopore Sensors for Biochemical Sensing Applications

Li,

Huang,

Wang

et al. 2024

Acc. Mater. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Ions, small molecules, and biomacromolecules are important components of the human body. They usually play important roles in various physiological and pathological events, showing a close relationship with human health. However, due to the ultralow concentrations of these substances and the presence of interfering chemicals, accurate and reliable measurement of these ions and molecules remains a huge challenge. Nanopore sensors, which combine nanofluidic and electrochemical technologies, have received a great deal of attention in recent years. Nanopore sensing is generally realized by measuring the electrochemical behaviors of ions in nanopores, which endows this technique with the advantages of high sensitivity, fast response, high sampling frequency, and experimental simplicity. In addition, owing to the confinement effect, the interaction between analytes and the nanopore is greatly enhanced, which can further improve the sensing sensitivity, even achieving single-entity analysis. With the development of materials science, micro/ nanoprocessing technologies, and mass transport theories at the nanoscale, nanopore sensors have established themselves as a promising tool for the analysis of ions, biomolecules, and nanoparticles. Nanopore materials, as the core of nanopore sensors, can be classified into three categories based on the pore structure: single nanopores, arrayed nanopores, and composite nanopores. Single nanopores include two-dimensional (2D) material based single nanopores and glass/quartz nanopipettes. The single-pore structure can offer high sensitivity and spatial resolution, making single nanopores suitable for singleentity analysis. Arrayed nanopores consist of a large number of orderly arranged pores, generally including polymer nanopores and metal oxide nanopores. Arrayed-nanopore sensors possess advantages, including easy preparation, low cost, and high throughput, making them widely applicable in biochemical and environmental analysis. Composite nanopores, on the other hand, combine nanopores with other materials, such as conductive polymers and plasmonic metals, which can further enhance the sensitivity and accuracy of nanopore sensing. Through introducing recognition elements into these nanopores, the interaction between the analyte and the recognition elements can produce predictable changes in the nanopore properties, such as diameter, pore shape, surface charge, and wettability, resulting in readable changes in ion-current signals.In this Account, we summarize the recent advancements in nanopore materials, nanopore-sensing mechanisms, and practical nanopore sensing applications, which are mainly based on work published by our group. We first briefly introduce single-, arrayed-, and composite-nanopore materials and their corresponding fabrication methods and then summarize the functionalization techniques employed to incorporate recognition sites within the nanopores. Then, we provide a glimpse of the fundamentals of nanopore sensing, inc...

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Section: Advancements In Nanopore Sensorsmentioning

confidence: 94%

Developing Solid-State Single-, Arrayed-, and Composite-Nanopore Sensors for Biochemical Sensing Applications

Li,

Huang,

Wang

et al. 2024

Acc. Mater. Res.

Self Cite

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“…[112] As shown in Figure 11b, Ji and Wang et al combined SERS with a goldcoated nanopipette to obtain direct insight into protein segments, which shed light on nanopipette-based protein sequencing. [113]…”

Section: Optical Methodsmentioning

confidence: 99%

Section: Other Applicationsmentioning

confidence: 99%

Solid‐State Glass Nanopipettes: Functionalization and Applications

Wang,

Tang,

Qiu

et al. 2024

Chemistry A European J

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Solid‐state glass nanopipettes provide a promising confined space that offers several advantages such as controllable size, simple preparation, low cost, good mechanical stability, and good thermal stability. These advantages make them an ideal choice for various applications such as biosensors, DNA sequencing, and drug delivery. In this review, we first delve into the functionalized nanopipettes for sensing various analytes and the methods used to develop detection means with them. Next, we provide an in‐depth overview of the advanced functionalization methodologies of nanopipettes based on diversified chemical kinetics. After that, we present the latest state‐of‐the‐art achievements and potential applications in detecting a wide range of targets, including ions, molecules, biological macromolecules, and single cells. We examine the various challenges that arise when working with these targets, as well as the innovative solutions developed to overcome them. The final section offers an in‐depth overview of the current development status, newest trends, and application prospects of sensors. Overall, this review provides a comprehensive and detailed analysis of the current state‐of‐the‐art functionalized nanopipette perception sensing and development of detection means and offers valuable insights into the prospects for this exciting field.

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“…Integration of SERS and nanopore could directly facilitate further generation of sequencing and the development of molecular dynamic research and related fields, such as biocatalysts and drug development . However, the efficiency of plasmonic coupling of the nanopore structure are still not high enough to provide sufficient fingerprint information on molecules in the nanopore, − which demands rational design of nanopore structures …”

Section: Challenges and Opportunitiesmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy: Current Understanding, Challenges, and Opportunities

Ma,

Pan,

Wang

et al. 2024

ACS Nano

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While surface-enhanced Raman spectroscopy (SERS) has experienced substantial advancements since its discovery in the 1970s, it is an opportunity to celebrate achievements, consider ongoing endeavors, and anticipate the future trajectory of SERS. In this perspective, we encapsulate the latest breakthroughs in comprehending the electromagnetic enhancement mechanisms of SERS, and revisit CT mechanisms of semiconductors. We then summarize the strategies to improve sensitivity, selectivity, and reliability. After addressing experimental advancements, we comprehensively survey the progress on spectrum−structure correlation of SERS showcasing their important role in promoting SERS development. Finally, we anticipate forthcoming directions and opportunities, especially in deepening our insights into chemical or biological processes and establishing a clear spectrum−structure correlation.

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Single Molecule Protein Segments Sequencing by a Plasmonic Nanopore

Cited by 16 publications

References 57 publications

Developing Solid-State Single-, Arrayed-, and Composite-Nanopore Sensors for Biochemical Sensing Applications

Developing Solid-State Single-, Arrayed-, and Composite-Nanopore Sensors for Biochemical Sensing Applications

Solid‐State Glass Nanopipettes: Functionalization and Applications

Surface-Enhanced Raman Spectroscopy: Current Understanding, Challenges, and Opportunities

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