Single-molecule nanopore sensing of actin dynamics and drug binding

Wang, Xiaoyi; Wilkinson, Mark D.; Lin, Xiaoyan; Ren, Ren; Willison, Keith R.; Ivanov, Aleksandar P.; Baum, Jake; Edel, Joshua B.

doi:10.1039/c9sc05710b

Cited by 39 publications

(45 citation statements)

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“…[111][112][113][114] As such, nanopipettes have been used for the detection of a range of analyte molecules including nucleic acids, proteins, and nucleic acid protein complexes. 23,25,[115][116][117][118][119][120] Nevertheless, their wider deployment is limited by difficulties in the production of pore arrays and nanopipettes with reproducible pore sizes.…”

Section: Solid State Nanoporesmentioning

confidence: 99%

In situsolid-state nanopore fabrication

et al. 2021

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Section: Solid State Nanoporesmentioning

confidence: 99%

In situsolid-state nanopore fabrication

et al. 2021

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“…[16][17][18][19][20][21][22][23] However, detection of proteins remains challenging due to fast analyte transport, low capture rates, and the need for relatively high protein concentration. [24,25] To date, a number of valuable approaches have emerged for nanopore based protein sensing. For example, the use of high salt conditions to slow down the translocation, or the use of high bandwidth instruments to resolve the fast transients.…”

Section: Doi: 101002/smtd202000356mentioning

confidence: 99%

Selective Sensing of Proteins Using Aptamer Functionalized Nanopore Extended Field‐Effect Transistors

et al. 2020

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The ability to sense proteins and protein-related interactions at the singlemolecule level is becoming of increasing importance to understand biological processes and diseases better. Single-molecule sensors, such as nanopores have shown substantial promise for the label-free detection of proteins; however, challenges remain due to the lack of selectivity and the need for relatively high analyte concentrations. An aptamer-functionalized nanopore extended field-effect transistor (nexFET) sensor is reported here, where protein transport can be controlled via the gate voltage that in turn improves single-molecule sensitivity and analyte capture rates. Importantly, these sensors allow for selective detection, based on the choice of aptamer chemistry, and can provide a valuable addition to the existing methods for the analysis of proteins and biomarkers in biological fluids.

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“…[ 8,11,12,79 ] Besides, methods that rapidly differentiate various dynamic states of proteins with low‐cost will greatly benefit drug design industry. [ 80 ] However, nowadays, the sensitivity of solid‐state nanopore sensors, which is characterized by signal to noise ratio (SNR = Δ I signal / I RMS ), is far from meeting the expectations. Here, we review three key physical factors that govern the sensitivity of protein sensing on the solid‐state nanopore platform: the limited temporal resolution, the electrical noise, and amplitude of current blockade, and then summarize the efforts to push the limits of sensitivity.…”

Section: Sensitivitymentioning

confidence: 99%

Four Aspects about Solid‐State Nanopores for Protein Sensing: Fabrication, Sensitivity, Selectivity, and Durability

Tong

Zhao

2020

Adv Healthcare Materials

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Solid‐state nanopores are a mimic of innate biological nanopores embedded on lipid membranes. They are fabricated on thin suspended layers of synthetic materials that provide superior thermal, mechanical, chemical stability, and geometry flexibility. As their counterpart biological nanopores reach the goal of DNA sequencing and become commercial, solid‐state nanopores thrive in aspects of protein sensing and have become an important research component for clinical diagnostic technologies. This review focuses on resistive pulse sensing modes, which are versatile for low‐cost, portable sensing devices and summarizes four main aspects toward commercially available resistive pulse‐based protein sensing techniques using solid‐state nanopores. In each aspect of fabrication, sensitivity, selectivity, and durability, brief fundamentals are introduced and the challenges and improvements are discussed. The rapid advance of a practical technique requires greater multidisciplinary cooperation. The review aims at clarifying existing obstacles in solid‐state nanopore based protein sensing, intriguing readers with existing solutions and finally encouraging multidisciplinary researchers to advance the development of this promising protein sensing methodology.

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Single-molecule nanopore sensing of actin dynamics and drug binding

Abstract: Nanopipettes were used for real-time investigation into actin dynamics and drug binding at single-molecule resolution, showing promise for a better understanding of the mechanism of protein–protein interactions and drug discovery.

Cited by 39 publications

References 50 publications

In situsolid-state nanopore fabrication

In situsolid-state nanopore fabrication

Selective Sensing of Proteins Using Aptamer Functionalized Nanopore Extended Field‐Effect Transistors

Four Aspects about Solid‐State Nanopores for Protein Sensing: Fabrication, Sensitivity, Selectivity, and Durability

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