Single molecule laser spectroscopy

Atta, Don Van; Okasha, Aly

doi:10.1016/j.saa.2014.07.085

Cited by 35 publications

(15 citation statements)

References 83 publications

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“…The other sources of errors may result from the chemical instability of fluorophore and/or sample optical properties altered by the presence of liposomes [ 6 ]. In a typical fluorescence correlation spectroscopy experiment the focal volume is determined using the water-soluble dye such as Rhodamine or Alexa 488 [ 17 ]. In this approach it is assumed that the water-soluble fluorescence dye diffusion coefficient is known in advance and that the photo-bleaching effect is negligible.…”

Section: Resultsmentioning

confidence: 99%

The Effect of a Fluorophore Photo-Physics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation Spectroscopy

et al. 2015

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Fluorescence Correlation Spectroscopy (FCS) is a technique, which allows determination of the diffusion coefficient and concentration of fluorescent objects suspended in the solution. The measured parameter is the fluctuation of the fluorescence signal emitted by diffusing molecules. When 100 nm DOPC vesicles labeled with various fluorescent dyes (Fluorescein-PE, NBD-PE, Atto488 DOPE or βBodipy FL) were measured, different values of diffusion coefficients have been obtained. These diffusion coefficients were different from the expected values measured using the dynamic light scattering method (DLS). The FCS was initially developed for solutions containing small fluorescent molecules therefore the observed inconsistency may result from the nature of vesicle suspension itself. The duration of the fluorescence signal may depend on the following factors: the exposure time of the labeled object to the excitation beam, the photo-physical properties (e.g., stability) of a fluorophore, the theoretical model used for the calculations of the diffusion coefficient and optical properties of the vesicle suspension. The diffusion coefficients determined for differently labeled liposomes show that its dependence on vesicle size and quantity of fluorescent probed used for labeling was significant demonstrating that the fluorescence properties of the fluorophore itself (bleaching and/or blinking) were critical factors for a correct outcome of FCS experiment. The new, based on combined FCS and DLS measurements, method for the determination of the focal volume prove itself to be useful for the evaluation of a fluorescence dye with respect to its applicability for FCS experiment.Electronic supplementary materialThe online version of this article (doi:10.1007/s10895-015-1752-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

The Effect of a Fluorophore Photo-Physics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation Spectroscopy

et al. 2015

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“…The mathematical development of the modeling ideas [4][5][6][7] is the basis for the collective model which has been particularly successful for A=24, 150<A<190 and A>230. The collective model could describe both the rotational states and the vibrational states, in molecular spectra, [8][9][10] one could easily see the evidences about three types of electronic excitation [11][12][13] through the electronic transitions, vibrational levels transitions and/or rotational states. On the other hand, inside the nuclei, one could be easily notice that the nucleons suffer the same transitions.…”

Section: Introductionmentioning

confidence: 99%

Particular Investigation of Energy Backbending Phenomena for Even-Even Nuclei 102Mo and 102Pd Modeling Approach

ELkhamisy

Atta

2020

International Journal of Advanced Scientific Research and Innov

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“…What we desire is not only to map the electron density around the studied molecules, but to determine the MEP surface. The characterization techniques of the polymers and nanostructures in scientific research work are depending mainly on the molecular spectroscopic tools like FTIR and Raman [28,29], together with the imaging techniques like electron microscopy and optical microscopic tools [30,31]. Molecular modeling has its importance that leads to understanding the recorded spectra and the detected images.…”

Section: Introductionmentioning

confidence: 99%

Molecular electrostatic potential mapping for PANI emeraldine salts and PANI@Ag core-shell

et al. 2019

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T HE quantum mechanical calculations have been utilized using the density function theory (DFT) byB3LYP method on the basis set 6-31g(d,p) to calculate the single point energy of Polyaniline emeraldine nitrate salt (PANI-nitrate). Polyaniline emeraldine sulphate (PANI-Sulphate) was also calculated on the same level. At the end the Ag@PANI-nitrate core-shell was calculated on the same level of calculations. For the previously named structures,the total dipole moment vector of every molecule was also calculated in addition to the energy band gap between the highest occupied molecular orbital (HOMO) and the lowest occupied molecular orbital (LUMO) and the distribution of the Electrostatic potential. More investigations for the studied molecules have been done in the presence of water molecules.

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Single molecule laser spectroscopy

Cited by 35 publications

References 83 publications

The Effect of a Fluorophore Photo-Physics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation Spectroscopy

The Effect of a Fluorophore Photo-Physics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation Spectroscopy

Particular Investigation of Energy Backbending Phenomena for Even-Even Nuclei 102Mo and 102Pd Modeling Approach

Molecular electrostatic potential mapping for PANI emeraldine salts and PANI@Ag core-shell

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