Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons

Godó, Soma; Barabás, Katalin; Lengyel, Ferenc; Ernszt, Dávid; Kovács, Tamás; Kecskés, Miklós; Varga, Csaba; Jánosi, Tibor Z.; Makkai, Géza; Kovács, Gergely; Orsolits, Barbara; Fujiwara, Takeshi; Kusumi, Akihiro; Ábrahám, István M.

doi:10.3389/fcell.2021.708715

Cited by 3 publications

(7 citation statements)

References 74 publications

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“…On one hand, the MLE does not reveal any significant effect due to E2 treatment, on the other hand the application of E2 significantly decreases the diffusion coefficients based on statistics on the MSD results. Previous study ( Godó et al, 2021 ) has shown that GPER1 internalization depletes the GPER1 which is crucial for the effectiveness of E2 in soma, indicating the propriety of MLE based result.…”

Section: Resultsmentioning

confidence: 84%

“…The result of MLE can be surprising as the lower E2 concentration (100 pM) evoked a significant decrease of the diffusion coefficients, while the administration of the higher dose of E2 (100 nM) did not induce any change. This effect was previously investigated ( Godó et al, 2021 ) and it was revealed that the difference may be the consequence of GPER1 internalization in the soma induced by 100 nM E2. It was also demonstrated that both ERβ and GPER1 are required for the effect of E2.…”

Section: Resultsmentioning

confidence: 93%

“…To detect GluR2-AMPAR molecules in the plasma membranes of differentiated PC12 (dPC12) ( Godó et al, 2021 ), live-cell immunofluorescent labeling was performed. Before single-molecule imaging, dPC12 were incubated with ATTO 488-labeled antibodies directed against the extracellular N-terminal domain of rat GluR2 (1:100, Alomone Labs Cat #: AGC-005-AG) in dRPMI cell culture medium at 37°C for 6 min.…”

Section: Methodsmentioning

confidence: 99%

“…Accordingly, measuring the diffusion parameters of the AMPARs can provide a better understanding of the non-classical E2 effects on learning and memory processes ( Godó et al, 2021 ). Therefore, it is crucial to improve currently available methods to analyze membrane receptor movements.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies ( Barabas et al, 2021 ; Godó et al, 2021 ) on lateral movement of receptors in the plasma membrane have demonstrated the value of the data extracted from SMD. SMD is a technique that can identify individual molecules and create the trajectories of these particles for detailed analysis.…”

Section: Introductionmentioning

confidence: 99%

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Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

Makkai

Ábrahám²,

Barabás³

et al. 2023

Front. Neuroinform.

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The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a deeper understanding of the underlying mechanisms of non-classical estradiol actions can be achieved by investigating receptor dynamics. Diffusion coefficient is a crucial and widely used parameter to characterize the movement of receptors in the cell membrane. The aim of this study was to investigate the differences between maximum likelihood-based estimation (MLE) and mean square displacement (MSD) based calculation of diffusion coefficients. In this work we applied both MSD and MLE to calculate diffusion coefficients. Single particle trajectories were extracted from simulation as well as from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor tracking in live estradiol-treated differentiated PC12 (dPC12) cells. The comparison of the obtained diffusion coefficients revealed the superiority of MLE over the generally used MSD analysis. Our results suggest the use of the MLE of diffusion coefficients because as it has a better performance, especially for large localization errors or slow receptor movements.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

Makkai

Ábrahám²,

Barabás³

et al. 2023

Front. Neuroinform.

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GLUT4 dispersal at the plasma membrane of adipocytes: a super-resolved journey

Geiser,

Foylan,

Tinning

et al. 2023

Bioscience Reports

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In adipose tissue, insulin stimulates glucose uptake by mediating the translocation of GLUT4 from intracellular vesicles to the plasma membrane. In 2010, insulin was revealed to also have a fundamental impact on the spatial distribution of GLUT4 within the plasma membrane, with the existence of two GLUT4 populations at the plasma membrane being defined: 1) as stationary clusters and 2) as diffusible monomers. In this model, in the absence of insulin, plasma membrane-fused GLUT4 are found to behave as clusters. These clusters are thought to arise from an exocytic event that retains GLUT4 at the fusion site; this has been proposed to function as an intermediate hub between GLUT4 exocytosis and re-internalisation. By contrast, insulin stimulation induces the dispersal of GLUT4 clusters into monomers and favors a distinct type of GLUT4-vesicle fusion event, known as fusion-with-release exocytosis. Here, we review how super-resolution microscopy approaches have allowed investigation of the characteristics of plasma membrane-fused GLUT4 and further discuss regulatory step(s) involved in the GLUT4 dispersal machinery, introducing the scaffold protein EFR3 which facilitates localisation of phosphatidylinositol 4-kinase type IIIα (PI4KIIIα) to the cell surface. We consider how dispersal may be linked to the control of transporter activity, consider whether macro-organisation may be a widely used phenomenon to control proteins within the plasma membrane, and speculate on the origin of different forms of GLUT4-vesicle exocytosis.

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Estradiol and Estrogen-like Alternative Therapies in Use: The Importance of the Selective and Non-Classical Actions

et al. 2022

Self Cite

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Estrogen is one of the most important female sex hormones, and is indispensable for reproduction. However, its role is much wider. Among others, due to its neuroprotective effects, estrogen protects the brain against dementia and complications of traumatic injury. Previously, it was used mainly as a therapeutic option for influencing the menstrual cycle and treating menopausal symptoms. Unfortunately, hormone replacement therapy might be associated with detrimental side effects, such as increased risk of stroke and breast cancer, raising concerns about its safety. Thus, tissue-selective and non-classical estrogen analogues have become the focus of interest. Here, we review the current knowledge about estrogen effects in a broader sense, and the possibility of using selective estrogen-receptor modulators (SERMs), selective estrogen-receptor downregulators (SERDs), phytoestrogens, and activators of non-genomic estrogen-like signaling (ANGELS) molecules as treatment.

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Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons

Cited by 3 publications

References 74 publications

Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

GLUT4 dispersal at the plasma membrane of adipocytes: a super-resolved journey

Estradiol and Estrogen-like Alternative Therapies in Use: The Importance of the Selective and Non-Classical Actions

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