Single-Molecule Fret Detects Intermediates and Fast Dynamics of DNA Holliday Junctions

Abstract

that melt at high temperatures (greater than 80 oC). This function is developed using a three-state model that approximates the ensemble of partially fluorescent premelted states as single state. The utility of this model is demonstrated by the extraction of melting temperatures from a set of synthesized DNA oligomers in both macro-and microscale environments to calibrate laser-heating for microdroplet PCR.