Single-Molecule Force Spectroscopy Studies on Sumo Polyproteins Reveal their Mechanical Flexibility

Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy

“…In an AFS experiment proteins of interest are deposited onto a surface and, after a protein comes into contact with the probe and is mechanically stretched between the surface and the probe, the forces used to unfold the proteins to an unstructured conformation can be directly determined (reviewed in refs − ). Such experiments can provide important insights into the energetics of that protein’s structure and protein folding or unfolding behavior. − …”