Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

Neuman, Keir C.; Nagy, Attila

doi:10.1038/nmeth.1218

Cited by 2,085 publications

(1,863 citation statements)

References 167 publications

(197 reference statements)

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“…Such applications would facilitate single molecule research by reducing the extensive labor requirements impeding some of the well-known technologies for precise physical manipulation of single molecule structures such as atomic force microscope (AFM), optical and magnetic tweezers. 53 …”

Section: Resultsmentioning

confidence: 99%

Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage

et al. 2013

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We demonstrate temperature-controlled encapsulation and release of the enzyme horseradish peroxidase using a preassembled and covalently closed three-dimensional DNA cage structure as a controllable encapsulation device. The utilized cage structure was covalently closed and composed of 12 double-stranded B-DNA helices that constituted the edges of the structure. The double stranded helices were interrupted by short single-stranded thymidine linkers constituting the cage corners except for one, which was composed by four 32 nucleotide long stretches of DNA with a sequence that allowed them to fold into hairpin structures. As demonstrated by gel-electrophoretic and fluorophore-quenching experiments this design imposed a temperature-controlled conformational transition capability to the structure, which allowed entrance or release of an enzyme cargo at 37 °C while ensuring retainment of the cargo in the central cavity of the cage at 4 °C. The entrapped enzyme was catalytically active inside the DNA cage and was able to convert substrate molecules penetrating the apertures in the DNA lattice that surrounded the central cavity of the cage.

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Section: Resultsmentioning

confidence: 99%

Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage

et al. 2013

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“…P recise recognition mechanisms of biological molecules offer a tremendous potential for the assembly [1][2][3] , imaging 4,5 and analysis of complex biological materials 6 . Single-molecule force spectroscopy (SMFS) experiments with the atomic force microscope (AFM) 7 have demonstrated the potential of intermolecular forces to be used for chemically specific nanoscale imaging 8 , manipulation 9 , directed assembly 10 , and biophysical studies of deformation and failure of biomolecules 11 .…”

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confidence: 99%

A nanomechanical interface to rapid single-molecule interactions

Dong

Şahin

2011

Nat Commun

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single-molecule techniques provide opportunities for molecularly precise imaging, manipulation, assembly and biophysical studies. owing to the kinetics of bond rupture processes, rapid single-molecule measurements can reveal novel bond rupture mechanisms, probe singlemolecule events with short lifetimes and enhance the interaction forces supplied by single molecules. Rapid measurements will also increase throughput necessary for technological use of single-molecule techniques. Here we report a nanomechanical sensor that allows single-molecule force spectroscopy on the previously unexplored microsecond timescale. We probed bond lifetimes around 5 µs and observed significant enhancements in molecular interaction forces. our loading-rate-dependent measurements provide experimental evidence for an additional energy barrier in the biotin-streptavidin complex. We also demonstrate quantitative mapping of rapid single-molecule interactions with high spatial resolution. This nanomechanical interface may allow studies of molecular processes with short lifetimes and development of novel biological imaging, single-molecule manipulation and assembly technologies.

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“…With a similar concept to optical tweezers, magnetic tweezers use a pair of permanent magnets to generate a constant magnetic force to move or rotate a paramagnetic particle due to the large characteristic length of the magnetic field gradient (19). Detailed working principle for these force spectroscopy techniques and their applications to single molecule studies where they have made the most impact have been well described in other reviews (20). In combination with microfluidics, one area where field gradient methods have contributed in recent years is in cell separation applications, where cells can be handled as objects to be moved around (21)(22)(23)(24).…”

Section: Feel the Force: Overview Of Field Gradient Methodsmentioning

confidence: 99%

Biophysical Tools for Cellular and Subcellular Mechanical Actuation of Cell Signaling

Liu

2016

Biophysical Journal

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The ability to spatially control cell signaling can help resolve fundamental biological questions. Optogenetic and chemical dimerization techniques along with fluorescent biosensors to report cell signaling activities have enabled researchers to both visualize and perturb biochemistry in living cells. A number of approaches based on mechanical actuation using forcefield gradients have emerged as complementary technologies to manipulate cell signaling in real time. This review covers several technologies, including optical, magnetic, and acoustic control of cell signaling and behavior and highlights some studies that have led to novel insights. I will also discuss some future direction on repurposing mechanosensitive channel for mechanical actuation of spatial cell signaling.

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Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

Cited by 2,085 publications

References 167 publications

Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage

Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage

A nanomechanical interface to rapid single-molecule interactions

Biophysical Tools for Cellular and Subcellular Mechanical Actuation of Cell Signaling

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