Single Molecule Enzymology Finds Its Stride

Perkel, Jeffrey M.

doi:10.2144/000114337

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Single-molecule enzymology, which focuses on studying single enzyme molecules instead of their large ensembles, is increasing in popularity [1,2]. This approach utilizes socalled molecular detectors, which allow researchers to investigate enzymes with ultra-high sensitivity [3]: atomic force microscopes [4][5][6][7], nanoelectronic detectors [8][9][10][11], nanoporebased sensors [12,13], total internal reflection microscopes [2] etc.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Incubation near an Inversely Oriented Square Pyramidal Structure on Adsorption Properties of Horseradish Peroxidase

Ivanov

Tatur²,

Pleshakova

et al. 2022

Applied Sciences

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The incubation of a solution of horseradish peroxidase (HRP) enzyme either below the apex or near the base of an inversely oriented square pyramid (inverted square pyramid; ISP) has been found to influence the enzyme’s aggregation and adsorption properties. The HRP enzyme is used herein as a model object due to its importance in analytical chemistry applications. Atomic force microscopy (AFM) is employed to investigate the HRP’s adsorption on mica substrates at the single-molecule level. Conventional spectrophotometry is used in parallel as a reference method for the determination of the HRP’s enzymatic activity. Using AFM, we reveal a significant change in the adsorption properties of HRP on mica substrates after the incubation of the HRP solutions either above the base or below the apex of the ISP in comparison with the control HRP solution. The same situation is observed after the incubation of the enzyme solution above the center of the ISP’s base. Here, the enzymatic activity of HRP remained unaffected in both cases. Since pyramidal structures of positive and inverted orientation are employed in biosensor devices, it is important to take into account the results obtained herein in the development of highly sensitive biosensor systems, in which pyramidal structures are employed as sensor (such as AFM probes) or construction elements.

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Section: Introductionmentioning

confidence: 99%

The Effect of Incubation near an Inversely Oriented Square Pyramidal Structure on Adsorption Properties of Horseradish Peroxidase

Ivanov

Tatur²,

Pleshakova

et al. 2022

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…Single-molecule enzymology, focusing on studying single enzyme molecules instead of their large ensembles, becomes an increasing popularity [1,2]. This approach utilizes so-called molecular detectors, which allow the researchers to investigate enzymes with ultra-high sensitivity [3]: atomic force microscopes [4][5][6][7], nanoelectronic detectors [8][9][10][11], nanopore-based sensors [12][13], total internal re ection microscopes [2] etc.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Incubation Near an Inverted Pyramidal Structure on Adsorption Properties of an Enzyme

Ivanov

Tatur²,

Pleshakova

et al. 2022

Preprint

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The incubation of a solution of horseradish peroxidase (HRP) enzyme near an inverted pyramid (IP) has been found to influence the enzyme's aggregation and adsorption properties. Atomic force microscopy (AFM) has been employed to study the adsorption of HRP onto mica substrates at the single-molecule level, while spectrophotometry has been used as a reference method for the estimation of its enzymatic activity. By AFM, we have revealed an increase in the relative content of HRP oligomers adsorbed onto mica substrates after the incubation of the HRP solutions below the IP's apex in comparison with the control HRP solution. The same situation has been observed after the incubation of the enzyme solution above the center of the IP's base. At that, the enzymatic activity of HRP remained virtually unchanged in the both cases. Since pyramidal structures of positive and inverted orientation are employed in biosensor devices, the results obtained in our study are important to be taken into account in the development of highly sensitive biosensor systems.

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Single Molecule Enzymology Finds Its Stride

Abstract: More techniques aimed at probing the nature of single molecules are being developed and advanced in biophysics labs. Jeffrey Perkel takes a look at the scientists leading the charge into the micro-world.

Cited by 2 publications

References 6 publications

The Effect of Incubation near an Inversely Oriented Square Pyramidal Structure on Adsorption Properties of Horseradish Peroxidase

The Effect of Incubation near an Inversely Oriented Square Pyramidal Structure on Adsorption Properties of Horseradish Peroxidase

The Effect of Incubation Near an Inverted Pyramidal Structure on Adsorption Properties of an Enzyme

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