Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

Hooley, E.; Paolucci, V.; Liao, Zhiyu; Carro‐Temboury, Miguel R.; Vosch, Tom

doi:10.1002/adom.201500249

Cited by 6 publications

(13 citation statements)

References 27 publications

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“…S4B) since the work-function of NCs did not match with that of the normal metallic state. Moreover, the size of Au NCs matches with Fermi wavelength of free electrons leading to the formation of quantized energy states and hence the energy levels of these NCs equal the molecule like behaviour2035. Thus it enables a multimodal sensing strategy for the detection of Cys over a wide concentration range including the biologically relevant concentration range under neutral, acidic and basic conditions.…”

Section: Resultsmentioning

confidence: 98%

“…Large number of reports are available in literature discussing the origin of fluorescence for Au NPs and Au NCs131415161718192021. Wu et al .…”

mentioning

confidence: 99%

“…In addition to the size reduction resulting in the formation of metal NCs, the emission properties can also be varied from UV to near IR (NIR) region. Particularly this shift in emission wavelength could be exploited in many different research fields, such as single molecular detection and recognizing fluorescence of metal NCs by using optical sensing research2021. Optically active noble metal nanomaterials such as Au and Ag are crucial for the construction of optical biosensors and these materials improve the properties in terms of cost, sensitivity, selectivity and miniaturization of devices etc 222324…”

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confidence: 99%

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Multimodal Sensing Strategy Using pH Dependent Fluorescence Switchable System

Muthurasu

Ganesh

2016

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Biomolecules assisted preparation of fluorescent gold nanoparticles (FL–Au NPs) has been reported in this work using glucose oxidase enzyme as both reducing and stabilizing agent and demonstrated their application through multimodal sensing strategy for selective detection of cysteine (Cys). Three different methods namely fluorescence turn OFF–ON strategy, naked eye detection and electrochemical methods are used for Cys detection by employing FL–Au NPs as a common probe. In case of fluorescence turn–OFF method a strong interaction between Au NPs and thiol results in quenching of fluorescence due to replacement of glucose oxidase by Cys at neutral pH. Second mode is based on fluorescence switch–ON strategy where initial fluorescence is significantly quenched by either excess acid or base and further addition of Cys results in appearance of rosy-red and green fluorescence respectively. Visual colour change and fluorescence emission arises due to etching of Au atoms on the surface by thiol leading to formation of Au nanoclusters. Finally, electrochemical sensing of Cys is also carried out using cyclic voltammetry in 0.1 M PBS solution. These findings provide a suitable platform for Cys detection over a wide range of pH and concentration levels and hence the sensitivity can also be tuned accordingly.

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Section: Resultsmentioning

confidence: 98%

“…Large number of reports are available in literature discussing the origin of fluorescence for Au NPs and Au NCs131415161718192021. Wu et al .…”

mentioning

confidence: 99%

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confidence: 99%

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Multimodal Sensing Strategy Using pH Dependent Fluorescence Switchable System

Muthurasu

Ganesh

2016

Sci Rep

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“…Although other DNA template sequences for AgNC formation were incorporated in this proposed system, unfortunately not all AgNCs were well formed under similar conditions or exhibited lower emission intensities, especially blue and green fluorescent emitting AgNCs. 26,27 This proved to be a bottleneck in generating a true multiplex (AgNC with four colors) system for DNA sensing.…”

Section: Discussionmentioning

confidence: 99%

DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping

et al. 2019

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The inherent ability of nucleic acids to recognize a complementary pair has gained wide popularity in DNA sensor applications. DNA molecules can be produced in bulk and easily incorporated with various nanomaterials for sensing applications. More complex designs and sophisticated DNA sensors have been reported over the years to allow DNA detection in a faster, cheaper, and more convenient manner. Here, we report a DNA sensor designed to function like a switch to turn "on" silver nanocluster (AgNC) generation in the presence of a specific DNA target. By defining the probe region sequence, we are able to tune the color of the AgNC generated in direct relation to the different targets. As a proof of concept, we used dengue RNA-dependent RNA polymerase conserved sequences from all four serotypes as targets. This method was able to distinguish each dengue serotype by generating the serotype-respective AgNCs. The DNA switch was also able to identify and amplify the correct target in a mixture of targets with good specificity. This strategy has a detection limit of between 1.5 and 2.0 µM depending on the sequence of AgNC. The DNA switch approach provides an attractive alternative for single-target or multiplex DNA detection.

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“…46 Time-resolved and polarizationresolved studies of single Ag N -DNAs within a polymer film find that Ag N -DNA spectral properties are sensitive to slight variations in local environment. 25,41,54 In one case, removing a terminal adenosine from the DNA template of an Ag 16 -DNA increases Stokes shift while leaving cluster geometry, peak absorbance energy, and quantum yield essentially unchanged. 14 Some Ag N -DNAs exhibit temperature-dependent excited state relaxation 9,14 while others do not.…”

Section: Introductionmentioning

confidence: 99%