“…While both gyrases appear to supercoil DNA in processive bursts, the EC enzyme, which derives from a fast-growing bacterium, supercoils at higher rates. Previous single-molecule work has shown that EC and Salmonella typhimurium gyrase are processive motors and that the Bacillus anthracis enzyme processively relaxes positive supercoils ( 36 , 42 ). The ∼5× faster EC gyrase speed than MTB may relate to the difference in doubling time for their respective host cells in vivo .…”