Single molecule analysis of the actomyosin motor

Overdamped mechanical model of myosin II

“…5. The free-energy difference that can be measured during lever arm rotation is around 3k B T ≈ 12 pN·nm [16]; this is also consistent with our numerical simulation.…”

Kinetic Characterization of Nonmuscle Myosin IIB at the Single Molecule Level

“…3f) were measured for the two molecules, which are within the ranges typically observed for myosin II molecules (for review, see Refs. 45,46). The unpaired Student's t test showed that the difference between the power stroke size values is not statistically significant (the two-tailed p value equals 0.8141).…”

Protein Linear Molecular Motor‐Powered Nanodevices

“…Since myosin II is a non-processive motor, the two heads act (quasi)independently. In support of this independent operation, it has been demonstrated [12] that one myosin head (actually the S1 unit) is sufficient to achieve motility, albeit at lower velocities. However, there are geometrical considerations that would suggest that the myosin heads cannot operate in a fully independent manner, and indeed there are studies [62,63] that have demonstrated a certain level of cooperativity.…”

“…The past four decades have witnessed extensive investigations into the molecular mechanisms that underpin the operation of these motors, which are central to biology. These fundamental investigations have been (i) experimental-aided by advances in molecular imaging, single molecule manipulation techniques, such as optical trapping, and development of motility assays, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] and (ii) theoretical-analyzing and attempting to explain the solely understanding their mechanisms per se. These devices are hybrids: consisting of naturally evolved protein motors, biochemically interfaced and housed in glass or polymeric microstructures, possibly with external (fluidic or EM) control of motility.…”