Single Marker Analysis and Mapping of QTLs Governing Fruit Weight in Eggplant (Solanum melongena L.)

Bhanushree, N; Saha, Partha; Tomar, B S; Krishnan, S. Gopala; Gurung, Bishal; Ghoshal, Chandrika; Munshi, A. D.

doi:10.1007/s40011-019-01149-y

Cited by 5 publications

(4 citation statements)

References 32 publications

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“…Pathogen race is an important factor in determining the resistance/susceptibility reaction of plant host. Previously in eggplant, BSA was used to identify the markers putatively linked to yield contributing traits and also to disease resistance of various diseases (Bhanushree et al, 2020; Pandiyaraj et al, 2019; Toppino et al, 2008). In the present study, we followed bulked segregant analysis to identify putatively linked markers to Phomopsis fruit rot resistance.…”

Section: Discussionmentioning

confidence: 99%

“…BSA is mainly based on the principle of selecting and grouping the plants from segregating populations with extreme phenotypes and testing for the differences in allele frequency between the population bulks. Previously in eggplant, BSA was used to identify the markers putatively linked to yield contributing traits and also to disease resistance of other diseases (Bhanushree et al, 2020; Pandiyaraj et al, 2019; Toppino et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Narayanswami,

Tomar,

Akhtar

et al. 2023

Plant Breeding

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The present investigation was carried out to decipher inheritance of resistance and to identify linked SSR markers for Phomopsis blight resistance in eggplant. An F2 population comprising 161 plants was developed from the cross of Pusa Kranti and BR‐40‐7. Genetic analysis was carried out using Chi square test. Artificial inoculation of fruits was carried out using pin prick method, and scoring was done as per the standard scoring scale. The F2 plants segregated into 92 susceptible (77—highly susceptible, 15—susceptible): 69 resistant (17—highly resistant, 27—resistant, 25—moderately resistant) suggesting complimentary epistasis with ratio of 9:7. To identify the putatively linked markers to resistance gene, parental polymorphic markers were subjected to bulk segregant analysis (BSA), and two markers (emk03O04 and emf11A03) could differentiate resistant and susceptible bulk and co‐segregated with resistance gene. The genetic distance between the identified markers was found to be 18.12 cM using QTL IciMapping V3.2 software depicting two new QTLs on chromosome number 6. The identified QTLs have great significant importance in marker assisted breeding programme.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Narayanswami,

Tomar,

Akhtar

et al. 2023

Plant Breeding

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“…To dissect this important trait for eggplant genetics and breeding, in this study we have combined classical forward genetics techniques with advanced association and sequencing analyses, using both experimental populations and germplasm materials to identify and validate a candidate gene controlling the synthesis of fruit peel chlorophyll. Up to now, the dissection of complex traits in eggplant has been usually performed using germplasm panels ( Portis et al., 2015 ) or temporary mapping populations, such as F 2 and BC 1 ( Barchi et al., 2012 ; Miyatake et al., 2016 ; Pandiyaraj et al., 2019 ; Bhanushree et al., 2020 ). These populations have the advantage of being easy and fast to develop but are not as powerful as the immortal ones to identify strong associations, candidate genes or causative SNPs, and accumulate fewer genetic recombination events, limiting the resolution for QTL detection ( Arrones et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

et al. 2022

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Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the fruit peel and used to identify candidate genes for this trait. A genome-wide association study (GWAS) performed with 420 MAGIC individuals revealed a major association on chromosome 8 close to a gene similar to APRR2. Two variants in SmAPRR2, predicted as having a high impact effect, were associated with the absence of fruit chlorophyll pigmentation in the MAGIC population, and a large deletion of 5.27 kb was found in two reference genomes of accessions without chlorophyll in the fruit peel. The validation of the candidate gene SmAPRR2 was performed by its sequencing in a set of MAGIC individuals and through its de novo assembly in 277 accessions from the G2P-SOL eggplant core collection. Two additional mutations in SmAPRR2 associated with the lack of chlorophyll were identified in the core collection set. The phylogenetic analysis of APRR2 reveals orthology within Solanaceae and suggests that specialization of APRR2-like genes occurred independently in Cucurbitaceae and Solanaceae. A strong geographical differentiation was observed in the frequency of predominant mutations in SmAPRR2, resulting in a lack of fruit chlorophyll pigmentation and suggesting that this phenotype may have arisen and been selected independently several times. This study represents the first identification of a major gene for fruit chlorophyll pigmentation in the eggplant fruit.

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“…SSR markers being multi‐allelic, co‐dominant, providing large genome coverage along with more resolution could be better applied in genetic diversity studies, gene tagging, linkage mapping and even in map‐based cloning (Huang et al, 2011; Stàgel et al, 2008). SSR markers were actively utilized in eggplant and related species to study their genetic diversity and relationships among them (Behera et al, 2006; Bhanushree et al, 2018; Nunome et al, 2003), and in studies deciphering genes of interest for the target traits (Bhanushree et al, 2023; Pandiyaraj et al, 2019). There are no such specific reports available showing the inheritance studies, markers identification as well as linkage map construction for the brinjal fruit‐bearing habit trait, Hence, this study was conducted and the identified marker could be used for marker‐assisted selection in eggplant breeding and we could locate potential gene involved.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis and molecular mapping of fruit‐bearing habit locus (Bh¹) in eggplant (Solanum melongena L.)

K. H.,

Saha,

Tomar

et al. 2024

Plant Breeding

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In eggplant, two kinds of fruit‐bearing habits can be observed, that is, cluster and solitary. The fruits grown in cluster are small in size but contribute towards yield and productivity. In this investigation, we have studied the genetics of fruit‐bearing habit and identified the SSR markers linked to this trait. Pusa Safed Baingan 1 (cluster bearer) and Pusa Hara Baingan 1 (solitary bearer) having contrasting traits were crossed to generate F1 (20 plants), F2 (215 plants), B1 (73 plants) and B2 (64 plants). Genetic analysis of the trait was carried out using Chi‐square test. The segregation of plants in F2 suggested recessive epistasis with ratio of 9:3:4 (solitary: mixed: cluster) indicating involvement of two genes for controlling the fruit‐bearing habit. The parental polymorphic 6 SSR markers were used for genotyping F2 plants and co‐segregated with the fruit‐bearing habit (Bh1) locus in 1:2:1. A linkage map covering 63.86 cm distance was developed, the SSR marker emf11A03 was closest with a distance of 4.37 cm, emk03O04 at 12.5 cm distance, and emf21O06 at 14.5 cm. The physical positions of the SSR markers emb01J19 and emf11A03 flanking Bh1 gene were located at 4.77 mb position of contig Sme2.5_05721.1 and 5.18 Mb position of contig Sme2.5_00994.1, respectively. The findings in the present study will be helpful in marker‐assisted breeding to enhance yield in eggplant.

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Single Marker Analysis and Mapping of QTLs Governing Fruit Weight in Eggplant (Solanum melongena L.)

Cited by 5 publications

References 32 publications

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

Genetic analysis and molecular mapping of fruit‐bearing habit locus (Bh¹) in eggplant (Solanum melongena L.)

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Single Marker Analysis and Mapping of QTLs Governing Fruit Weight in Eggplant (Solanum melongena L.)

Cited by 5 publications

References 32 publications

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

Genetic analysis and molecular mapping of fruit‐bearing habit locus (Bh1) in eggplant (Solanum melongena L.)

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Genetic analysis and molecular mapping of fruit‐bearing habit locus (Bh¹) in eggplant (Solanum melongena L.)