Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System

Hoehn, A.; Freeman, Jacob; Koenig, Paul; Stodieck, Louis S.; Vellone, Matthew; Williams, Shea; Feenstra, Wendy; Luis, Javier; Parrish, Joseph; Pretorius, Stephan; Renshaw, Robert; Sun, Liping; Dyble, Stephen; Searby, N. D.; Vandendriesche, Donald

doi:10.4271/2006-01-2212

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Before the project was completed, the requirements were modified and the CCU was replaced by 12 simpler units ( Figure 6) that take single cultures: 4 to fly, 4 for ground controls, and 4 for backups 136,137 . These Single Loop for Cell Culture (SLCCs) were delivered to NASA Ames in June, 2006, but have not been flown yet.…”

Section: On Board Experimentsmentioning

confidence: 99%

Diatoms in space: testing prospects for reliable diatom nanotechnology in microgravity

Gordon

Hoover²,

Tuszyński

et al. 2007

Instruments, Methods, and Missions for Astrobiology X

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The worldwide effort to grow nanotechnology, rather than use lithography, focuses on diatoms, single cell eukaryotic algae with ornate silica shells, which can be replaced by oxides and ceramics, or reduced to elemental silicon, to create complex nanostructures with compositions of industrial and electronics importance. Diatoms produce an enormous variety of structures, some of which are microtubule dependent and perhaps sensitive to microgravity. The NASA Single Loop for Cell Culture (SLCC) for culturing and observing microorganisms permits inexpensive, low labor inspace experiments. We propose to send up to the International Space Station diatom cultures of the three diatom species whose genomes are currently being sequenced, plus the giant diatoms of Antarctica (up to 6 mm length for a single cell) and the unique colonial diatom, Bacillaria paradoxa. Bacillaria cells move against each other in partial synchrony, like a sliding deck of cards, by a microfluidics mechanism. Will normal diatoms have aberrant patterns, shapes or motility compared to ground controls? The generation time is typically one day, so that many generations may be examined from one flight. Rapid, directed evolution may be possible running the SLCC as a compustat. The shell shapes and patterns are preserved in hard silica, so that the progress of normal and aberrant morphogenesis may be followed by drying samples on a moving filter paper "diatom tape recorder". With a biodiversity of 100,000 distinct species, diatom nanotechnology may offer a compact and portable nanotechnology toolkit for space exploration anywhere.

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Section: On Board Experimentsmentioning

confidence: 99%

Diatoms in space: testing prospects for reliable diatom nanotechnology in microgravity

Gordon

Hoover²,

Tuszyński

et al. 2007

Instruments, Methods, and Missions for Astrobiology X

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Science Research and Education Modules for the CGBA Spaceflight Incubator

Hoehn¹,

Countryman²,

Freeman³

et al. 2007

SAE Technical Paper Series

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Microbial Detection Array (MDA), a Novel Instrument for Unambiguous Detection of Microbial Metabolic Activity in Astrobiology Applications

Hoehn¹,

Lynch²,

Clawson³

et al. 2007

SAE Technical Paper Series

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MDA is designed as a test bed for an astrobiology field instrument to detect microbial metabolic activity in terrestrial or extraterrestrial geological soil samples. MDA employs electrochemical sensors in a unique differential chamber configuration, able to detect minute changes in the chemical composition between the two otherwise identical chambers. Both chambers are filled with identical autoclave-sterilized, sample-water mixtures. Only one of the chambers receives an additional minute, non-sterilized inoculation sample. Under the minimal assumptions that the geological sample contained nutrients (energy), organisms, and required water to initiate growth, the differential electrochemical measurements would now allow detection of metabolic activity, in addition to the electrochemical characterization of the soil samples in both chambers.

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Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System

Cited by 3 publications

References 6 publications

Diatoms in space: testing prospects for reliable diatom nanotechnology in microgravity

Diatoms in space: testing prospects for reliable diatom nanotechnology in microgravity

Science Research and Education Modules for the CGBA Spaceflight Incubator

Microbial Detection Array (MDA), a Novel Instrument for Unambiguous Detection of Microbial Metabolic Activity in Astrobiology Applications

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