Purpose: Laparoscopic cholecystectom y has been the standard of care for gallbladder diseases since the late 1980s. Many surgeons have rapidly adopted single-port laparoscopic cholecystectom y for gallbladder pathologies. The aim of the current study w as to analyze clinical outcom e in initial single-port laparoscopic cholecystectom y. Methods: Analysis of data from 206 consecutive single-port laparoscopic cholecystectom ies perform ed betw een M ay 2008 and Jun 2012 w as conducted retrospectively. W e divided the patients into four groups according to surgery period-period I (n=56), II (n=50), III (n=50), and IV (n=50), consecutively. During each procedure only one longitudinal transum bilical incision, 1.5 to 2.0 cm in length, w as m ade in order to access the abdom inal cavity. O ne of the various single-port trocars w as used for the procedure. Standard laparoscopic instrum ents w ere used in perform ance of cholecystectom y. Results: Patients' dem ographics did not differ am ong the groups. O f the 14 cases that w ere converted to conventional laparoscopic surgery, seven w ere part of period I, one of II, five of III, and one of IV. Mean operation tim e for single-port laparoscopic cholecystectom y in each group w as 71.6, 58.2, 69.1, and 53.3 m inutes, in order. There w ere tw o operative com plications in period I, w hich w ere m anaged successfully w ith laparoscopic surgery. N o statistical difference in hospital stay w as observed am ong the groups. Conclusion: Single-port laparoscopic cholecystectom y can be perform ed safely for various gallbladder lesions in selected cases, and the operation tim e im proved w ith accum ulation of cases.