Single human oocyte transcriptome analysis reveals distinct maturation stage‐dependent pathways impacted by age

Llonch, Sílvia; Barragán, Montserrat; Nieto, Paula; Mallol, Anna; Elosúa-Bayes, Marc; Lorden, Patricia; Ruiz, Sara; Zambelli, Filippo; Heyn, Holger; Vassena, Rita; Payer, Bernhard

doi:10.1111/acel.13360

Cited by 49 publications

(45 citation statements)

References 82 publications

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“…This hypothesis is supported by mouse studies showing an effect of obesity on oocyte polarization, reactive oxygen species levels and DNA methylation, including methylation of metabolism-related genes, such as the leptin promotor region [ 44 , 45 ]. In humans, it has been demonstrated that rising BMI affects regulation of oocyte RNA expression and oocyte metabolism [ 35 , 46 , 47 ]. Furthermore, the maternally-inherited genome passively demethylates with each cell-division, reaching the lowest level at the blastocyst stage, whereas the paternally-inherited genome actively demethylates within 8 h after fertilization [ 48 – 50 ].…”

Section: Discussionmentioning

confidence: 99%

Higher preconceptional maternal body mass index is associated with faster early preimplantation embryonic development: the Rotterdam periconception cohort

Duijn

Rousian

Hoek

et al. 2021

Reprod Biol Endocrinol

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Background Overweight and obesity affect millions of people globally, which has also serious implications for reproduction. For example, treatment outcomes after in vitro fertilisation (IVF) are worse in women with a high body mass index (BMI). However, the impact of maternal BMI on embryo quality is inconclusive. Our main aim is to study associations between preconceptional maternal BMI and morphokinetic parameters of preimplantation embryos and predicted implantation potential. In addition, associations with clinical IVF outcomes are investigated. Methods From a tertiary hospital, 268 women undergoing IVF or IVF with intracytoplasmic sperm injection (ICSI) were included; 143 normal weight, 79 overweight and 46 obese women. The embryos of these women were cultured in the EmbryoScope, a time-lapse incubator. The morphokinetic parameters of preimplantation embryos and predicted implantation potential, assessed by the KIDScore algorithm were longitudinally evaluated as primary and secondary outcomes, respectively. The tertiary outcomes included clinical outcomes, i.e., fertilization, implantation and live birth rate. Results After adjustment for patient- and treatment-related factors, we demonstrated in 938 embryos that maternal BMI is negatively associated with the moment of pronuclear appearance (βtPNa -0.070 h (95%CI -0.139, -0.001), p = 0.048), pronuclear fading (βtPNf -0.091 h (95%CI -0.180, -0.003), p = 0.043 and the first cell cleavage (βt2 -0.111 h (95%CI -0.205, -0.016), p = 0.022). Maternal BMI was not significantly associated with the KIDScore and tertiary clinical treatment outcomes. In embryos from couples with female or combined factor subfertility, the impact of maternal BMI was even larger (βtPNf -0.170 h (95%CI -0.293, -0.047), p = 0.007; βt2 -0.199 h (95%CI -0.330, -0.067), p = 0.003). Additionally, a detrimental impact of BMI per point increase was observed on the KIDScore (β -0.073 (se 0.028), p = 0.010). Conclusions Higher maternal BMI is associated with faster early preimplantation development. In couples with female or combined factor subfertility, a higher BMI is associated with a lower implantation potential as predicted by the KIDScore. Likely due to power issues, we did not observe an impact on clinical treatment outcomes. However, an effect of faster preimplantation development on post-implantation development is conceivable, especially since the impact of maternal BMI on pregnancy outcomes has been widely demonstrated.

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Section: Discussionmentioning

confidence: 99%

Higher preconceptional maternal body mass index is associated with faster early preimplantation embryonic development: the Rotterdam periconception cohort

Duijn

Rousian

Hoek

et al. 2021

Reprod Biol Endocrinol

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“…Similarly, a study based on 133 patients (aged 1–35 years) who underwent FP by ovarian tissue cryopreservation found that patients between menarche to 25 years could achieve the highest IVM rates, while women ≥30 years and pre-menarche girls (<6 years) obtained extremely low IVM rates (<10%) [ 134 ]. The changed oocyte maturation capacity in different ages may stem from the epigenetic changes [ 135 ] and differential gene expressions [ 136 , 137 ] of oocytes as they age. Besides, it has been proven that ovaries before puberty contain abnormal follicles with decreased follicle development [ 138 ].…”

Section: Woman’s Agementioning

confidence: 99%

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

et al. 2021

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In vitro maturation (IVM) of oocytes is a promising assisted reproductive technology (ART) deemed as a simple and safe procedure. It is mainly used in patients with impaired oocyte maturation and in fertility preservation for women facing the risk of losing fertility. However, to date, it is still not widely used in clinical practice because of its underperformance. The influencing factors, such as biphasic IVM system, culture medium, and the supplementation, have a marked effect on the outcomes of oocyte IVM. However, the role of different culture media, supplements, and follicular priming regimens in oocyte IVM have yet to be fully clarified and deserve further investigation.

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“…This provides arguments to use mitochondrial transfer to improve IVF pregnancy rates in elderly women [ 222 ], even if more data are necessary before an eventual implementation. Globally, age could impact all proteins [ 223 , 224 ] and aneuploidy is only the most accessible defect detected using conventional technologies. In horses, to the author’s knowledge, high throughput analysis of the oocyte is not yet available.…”

Section: Effects Of Maternal Environment In Equine and Humanmentioning

confidence: 99%

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Benammar

Derisoud

Vialard

et al. 2021

Animals

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Although there are large differences between horses and humans for reproductive anatomy, follicular dynamics, mono-ovulation, and embryo development kinetics until the blastocyst stage are similar. In contrast to humans, however, horses are seasonal animals and do not have a menstrual cycle. Moreover, horse implantation takes place 30 days later than in humans. In terms of artificial reproduction techniques (ART), oocytes are generally matured in vitro in horses because ovarian stimulation remains inefficient. This allows the collection of oocytes without hormonal treatments. In humans, in vivo matured oocytes are collected after ovarian stimulation. Subsequently, only intra-cytoplasmic sperm injection (ICSI) is performed in horses to produce embryos, whereas both in vitro fertilization and ICSI are applied in humans. Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. Embryo and oocyte cryopreservation has been mastered in humans, but not completely in horses. Finally, both species share infertility concerns due to ageing and obesity. Thus, reciprocal knowledge could be gained through the comparative study of ART and infertility treatments both in woman and mare, even though the horse could not be used as a single model for human ART.

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Single human oocyte transcriptome analysis reveals distinct maturation stage‐dependent pathways impacted by age

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 49 publications

References 82 publications

Higher preconceptional maternal body mass index is associated with faster early preimplantation embryonic development: the Rotterdam periconception cohort

Higher preconceptional maternal body mass index is associated with faster early preimplantation embryonic development: the Rotterdam periconception cohort

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

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