Single Gold@Silver Nanoprobes for Real-Time Tracing the Entire Autophagy Process at Single-Cell Level

Abstract

This article describes a multimodified core-shell gold@silver nanoprobe for real-time monitoring the entire autophagy process at single-cell level. Autophagy is vital for understanding the mechanisms of human pathologies, developing novel drugs, and exploring approaches for autophagy controlling. A major challenge for autophagy study lies in real-time monitoring. One solution might come from real-time detection of in situ superoxide radicals (O2(•-)), because it is the main regulator of autophagy. In this work, our proposed nanoprobes were etched by O2(•-) and gave a notable wavelength change in the plasmon resonance scattering spectra. Both the experimental and simulated results suggested the wavelength change rate correlated well with O2(•-) level. This response enabled its application in real-time in situ quantification of O2(•-) during autophagy course. More importantly, with the introduction of "relay probe" operation, two types of O2(•-)-regulating autophagy processes were successfully traced from the beginning to the end, and the possible mechanism was also proposed.

Imaging Local Heating and Thermal Diffusion of Nanomaterials with Plasmonic Thermal Microscopy

“…The higher efficiency is expected because the wavelength of the heating laser was close to the absorption peak of GNRs. 37 To confirm this observation, control experiments were carried out using 60 nmdiameter gold nanoparticles (GNPs), 540 nm silica nanoparticles (Silica NPs) and blank sample 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 camera (frame rate up to 120,000 fps) was used to capture fast transient heat diffusion process due to the heating of single GNRs.…”

Direct observation of single plasmonic metal nanoparticle reaction in microcolumn with chromatic‐aberration‐free LASER light‐sheet scattering imaging

“…In fact, due to the heterogeneous nucleation process, there is always a slight difference between the synthesized MNPs at the individual particle level. To explore the difference in crystal structure and surface energy, real‐time detection of the oxidation etching reaction of MNPs at the single particle level is significant . In this work, we selected AuNRs (37 × 70 nm) with different surface modifications as the model system of single MNP reaction study.…”

Developing Hollow-Channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes

“…The good spatial agreement of the signals observed in the dark-field and fluorescence microscopy and the absence of a dispersed fluorescence signal suggest the nanotags (HAuNFs@R6G@dBSA) possess good stability in the intracellular environment, without the detachment of R6G from the nanoconjugates. These observations demonstrate the capability of HAuNFs@R6G@dBSA as dark-field and fluorescent imaging nanoprobes, which would combine the advantages of excellent photo-stability (i.e., negligible photobleaching or photo-blinking) conferred by the scattering imaging based on novel metal nanoparticles [32], alongside the high spatial resolution and rich information at the subcellular level provided by (confocal) fluorescent imaging [33]. …”