Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection

Sato, Akio; Toyoshima, Koh-éi; Toki, H.; Ishibashi, Naoko; Asakawa, Kyosuke; Iwadate, Ayako; Kanayama, Tatsuya; Tobe, Hirofumi; Tsuji, Takashi

doi:10.1111/j.1346-8138.2012.01505.x

Cited by 14 publications

(27 citation statements)

References 32 publications

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“…6c). These results indicate that the bioengineered hair follicles have a piloerection ability that is comparable to that of natural follicles45. These findings indicated that our bioengineered follicles could induce selective connections with the appropriate types of muscles and nerve fibres, and they showed that these follicles were able to achieve piloerection through the rearrangement of stem cells and their niches (Fig.…”

Section: Resultssupporting

confidence: 57%

Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches

et al. 2012

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Organ replacement regenerative therapy is purported to enable the replacement of organs damaged by disease, injury or aging in the foreseeable future. Here we demonstrate fully functional hair organ regeneration via the intracutaneous transplantation of a bioengineered pelage and vibrissa follicle germ. The pelage and vibrissae are reconstituted with embryonic skin-derived cells and adult vibrissa stem cell region-derived cells, respectively. The bioengineered hair follicle develops the correct structures and forms proper connections with surrounding host tissues such as the epidermis, arrector pili muscle and nerve fibres. The bioengineered follicles also show restored hair cycles and piloerection through the rearrangement of follicular stem cells and their niches. This study thus reveals the potential applications of adult tissue-derived follicular stem cells as a bioengineered organ replacement therapy.

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Section: Resultssupporting

confidence: 57%

Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches

et al. 2012

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“…The hair follicles are major components of the cutaneous nerve network, and play a pivotal role in modulation of skin innervation. The transplanted adult hair follicle unit including functional APM can spontaneously induce the recovery of the neurofollicular and neuromuscular junctions22. Therefore, the incremental DNFL might contribute to the follicle cycling and APM function.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological Features and Immunohistochemical Alterations of Keratinocyte Proliferation, Melanocyte Density, Smooth Muscle Hyperplasia and Nerve Fiber Distribution in Becker's Nevus

et al. 2016

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BackgroundAlthough Becker's nevus (BN) is a relatively common disease, the systematic studies of clinicopathological and immunohistochemical results are poorly reported.ObjectiveTo investigate the clinicopathological features and immunohistochemical alterations of keratinocyte proliferation, melanocyte density, smooth muscle hyperplasia and nerve fiber distribution in BN.MethodsClinical and pathological data were collected in 60 newly-diagnosed BN cases. Immunohistochemical stain of Ki-67, Melan-A, keratin 15, smooth muscle actin and protein gene product 9.5 was performed in 21 cases.ResultsThe median diagnostic and onset age was 17 and 12 years, respectively. Skin lesions usually appeared on the upper trunk and upper limbs. The pathological features included the rete ridge elongation and fusion and basal hyperpigmentation. Epidermal Ki-67, Melan-A and keratin 15 expression and dermal nerve fiber length were significantly higher in lesional and perilesional skin than in normal skin (p<0.05~0.01), while smooth muscle actin expression was upregulated only in skin lesion (p<0.05).ConclusionAlthough the clinical diagnosis of BN is often straightforward, histopathology is helpful to differentiate from other pigmentary disorders. The hyperproliferation of keratinocytes, melanocytes, arrector pili muscle and dermal nerve fibers could be involved in the pathogenesis of BN.

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“…40 We further demonstrated that mature bioengineered hair follicles, but not the follicle germ generated by ectopic transplantation, could functionally replace orthotopic autologous hair follicle unit transplantation therapy, which had achieved clinical restoration of proper hair appearance by controlling hair type, hair density, and hair stream via the representation of natural hair orientation through the surgical implantation of hair follicles. 44 The bioengineered hair follicles reproduced the hair cycle through the rearrangement of stem cell niches for epithelial stem cells and mesenchymal precursor cells in the DP. The bioengineered hair follicle correctly connected autonomously to the arrector pili muscle and nerve fibers, and showed piloerection in the skin environment.…”

Section: Functional Hair Follicle Regenerationmentioning

confidence: 99%

Development and Prospects of Organ Replacement Regenerative Therapy

Hirayama

Oshima

Tsuji

2013

Cornea

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Current approaches for the development of regenerative therapies have been influenced by our understanding of embryonic development, stem cell biology, and tissue engineering technology. The ultimate goal of regenerative therapy is to develop fully functioning bioengineered organs to replace lost or damaged organs that result from disease, injury, or aging. Almost all organs including ectodermal organs, such as teeth, hair, salivary glands, and lacrimal glands, arise from organ germs induced by reciprocal epithelial-mesenchymal interactions in the developing embryo. A novel concept to generate a bioengineered organ is to recreate organogenesis and thereby develop fully functioning bioengineered organs from the resulting bioengineered organ germ generated via 3-dimensional cell manipulation using immature stem cells in vitro. We have previously developed a bioengineering method for forming a 3-dimensional organ germ in the early developmental stages, termed the "bioengineered organ germ method." Recently, we reported fully functioning bioengineered tooth replacements after transplantation of a bioengineered tooth germ or a mature tooth unit comprising the bioengineered tooth and periodontal tissues. This concept could be adopted to generate not only teeth but also bioengineered hair follicles, salivary glands, and lacrimal glands. These studies emphasize the potential for bioengineered organ replacement in future regenerative therapies. In this review, we will summarize the strategies and the recent progress of research and development for the establishment of organ replacement regenerative therapies.

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Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection

Cited by 14 publications

References 32 publications

Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches

Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches

Clinicopathological Features and Immunohistochemical Alterations of Keratinocyte Proliferation, Melanocyte Density, Smooth Muscle Hyperplasia and Nerve Fiber Distribution in Becker's Nevus

Development and Prospects of Organ Replacement Regenerative Therapy

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