Single fluorescence centers on the tips of crystal needles: First observation and prospects for application in scanning one-atom fluorescence microscopy

Sekatskiǐ, S. K.; Letokhov, V. S.

doi:10.1007/s003400050119

Cited by 31 publications

(15 citation statements)

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“…Such orientation dependence can potentially enable the study of protein folding and the environment effect on protein interactions. 266 Scanning FRET microscopy was developed with a SNOM fiber probe 271 and with an apertureless AFM tip. 272 In the fiber probe approach, a small crystal containing the donor or the acceptor was placed at the apex of the fiber probe.…”

Section: B Förster Resonance Energy Transfermentioning

confidence: 99%

“…272 In the fiber probe approach, a small crystal containing the donor or the acceptor was placed at the apex of the fiber probe. 271 In the apertureless approach, the AFM tip was coated with donor or acceptor molecules. 272 When the FRET-activated fiber probe or AFM tip scanned the sample, the complementary donor/acceptor deposited on the sample generated a FRET signal if it was within the Förster radius.…”

Section: B Förster Resonance Energy Transfermentioning

confidence: 99%

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Invited Review Article: Combining scanning probe microscopy with optical spectroscopy for applications in biology and materials science

Lucas

Riedo

2012

Review of Scientific Instruments

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This is a comprehensive review of the combination of scanning probe microscopy (SPM) with various optical spectroscopies, with a particular focus on Raman spectroscopy. Efforts to combine SPM with optical spectroscopy will be described, and the technical difficulties encountered will be examined. These efforts have so far focused mainly on the development of tip-enhanced Raman spectroscopy, a powerful technique to detect and image chemical signatures with single molecule sensitivity, which will be reviewed. Beyond tip-enhanced Raman spectroscopy and/or topography measurements, combinations of SPM with optical spectroscopy have a great potential in the characterization of structure and quantitative measurements of physical properties, such as mechanical, optical, or electrical properties, in delicate biological samples and nanomaterials. The different approaches to improve the spatial resolution, the chemical sensitivity, and the accuracy of physical properties measurements will be discussed. Applications of such combinations for the characterization of structure, defects, and physical properties in biology and materials science will be reviewed. Due to the versatility of SPM probes for the manipulation and characterization of small and/or delicate samples, this review will mainly focus on the apertureless techniques based on SPM probes.

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Section: B Förster Resonance Energy Transfermentioning

confidence: 99%

Section: B Förster Resonance Energy Transfermentioning

confidence: 99%

Invited Review Article: Combining scanning probe microscopy with optical spectroscopy for applications in biology and materials science

Lucas

Riedo

2012

Review of Scientific Instruments

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“…After the exposure to X‐rays, the LiF plate storing sample image is illuminated with blue light and the fluorescent CCs spatial distribution, which reproduces the absorption contrast image of the sample, is observed by an optical microscope in fluorescence mode. While using luminescent LiF as soft X‐ray imaging detector, the spatial resolution is limited by the image reading optical process; as a matter of fact, the intrinsic spatial resolution of LiF detectors is, in principle, limited only from the dimension of the produced CCs (a few nanometres; Sekatski & Letokhov, ). Anyway, by using as reading process of LiF luminescent detectors advanced optical microscopes, like confocal laser scanning microscope and scanning near‐field optical microscope, optical fluorescence images with micro‐ and nanometric spatial resolutions can be respectively obtained (Almaviva et al ., 2006; Ustione et al ., ).…”

Section: Methodsmentioning

confidence: 99%

Contact X‐ray microscopy of living cells by using LiF crystal as imaging detector

Reale

Bonfigli

Lai

et al. 2015

Journal of Microscopy

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In this paper, the use of lithium fluoride (LiF) as imaging radiation detector to analyse living cells by single-shot soft X-ray contact microscopy is presented. High resolved X-ray images on LiF of cyanobacterium Leptolyngbya VRUC135, two unicellular microalgae of the genus Chlamydomonas and mouse macrophage cells (line RAW 264.7) have been obtained utilizing X-ray radiation in the water window energy range from a laser plasma source. The used method is based on loading of the samples, the cell suspension, in a special holder where they are in close contact with a LiF crystal solid-state X-ray imaging detector. After exposure and sample removal, the images stored in LiF by the soft X-ray contact microscopy technique are read by an optical microscope in fluorescence mode. The clear image of the mucilaginous sheath the structure of the filamentous Leptolyngbya and the visible nucleolus in the macrophage cells image, are noteworthiness results. The peculiarities of the used X-ray radiation and of the LiF imaging detector allow obtaining images in absorption contrast revealing the internal structures of the investigated samples at high spatial resolution. Moreover, the wide dynamic range of the LiF imaging detector contributes to obtain high-quality images. In particular, we demonstrate that this peculiar characteristic of LiF detector allows enhancing the contrast and reveal details even when they were obscured by a nonuniform stray light.

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“…Nowadays, a variety of different implementations of SNOM exists. Starting from the visionary work of Synge in 1929 [25] in which the basic idea of apertured SNOM was presented, the concept of a microscopy technique based on the local interaction of light mediated by a nanoprobe has been extended including, but not limited at, Scattering-type SNOM (s-SNOM) [26], collection mode SNOM [27], illumination-mode SNOM [28], PSTM (Photon Scanning Tunnelling Microscopy) [29], coupled to several detection methods ranging from interferometric homodyne [30], heterodyne [31,32], to spectrometric (fluorescence [33,34] and Raman [35]). A comprehensive description of SNOM-related techniques is outside the scope of this work.…”

Section: Techniques For the Characterization Of Surfaces At The Nanosmentioning

confidence: 99%

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

Chiono

Descrovi

Sartori

et al. 2010

Scanning Probe Microscopy in Nanoscience and Nanotechnology 2

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New-generation biomaterials are information-rich and incorporate biologically active components derived from Nature. The chapter reviews current approaches for the realization of biomimetic coatings for tissue engineering applications, via functionalization with bioactive molecules such as native long chains of extracellular matrix (ECM) proteins as well as short peptide sequences derived from intact ECM proteins. Biomimetic materials provide cues for cell-matrix interactions, favouring cell adhesion, proliferation, spreading, and differentiation. The recently developed scanning probe techniques for optical and spectroscopic characterization of surfaces are also described, providing advanced topographical imaging and sensitive force measurements. The spectroscopic imaging of surfaces at the nanoscale provides insight into the function and structure of biomimetic molecules and represents a tool for tailoring the surface design.

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Single fluorescence centers on the tips of crystal needles: First observation and prospects for application in scanning one-atom fluorescence microscopy

Cited by 31 publications

References 0 publications

Invited Review Article: Combining scanning probe microscopy with optical spectroscopy for applications in biology and materials science

Invited Review Article: Combining scanning probe microscopy with optical spectroscopy for applications in biology and materials science

Contact X‐ray microscopy of living cells by using LiF crystal as imaging detector

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

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