Single-Fluorescence ATP Sensor Based on Fluorescence Resonance Energy Transfer Reveals Role of Antibiotic-Induced ATP Perturbation in Mycobacterial Killing

Abstract

Bioenergetics has emerged as a new paradigm for antituberculosis (anti-TB) drug discovery, and the cellular ATP level is the core indicator reflecting bacterial metabolic homeostasis. Although several bulk assays have been designed for the measurement of cellular ATP content, a more convenient strategy is required for real-time ATP measurement of single viable cells.

Antibacterial Activity and Mechanism of Action of Osthole against Listeria monocytogenes

“…ATP plays a key role in storing and transferring energy in cells and reveals the metabolic state of bacteria. It was reported that ATP fluctuations during antibiotic treatment could provide insight into drug efficacy . Consequently, intracellular and extracellular ATP levels of L.…”

“…Compared with the growth rate, the cellular ATP level could indicate the antibiotic efficacy more accurately. 41,43 For instance, it was reported that if cells treated with antibiotics such as streptomycin, rifampin, and ciprofloxacin, underwent a switch from a high to low intracellular ATP level, they would never recommence growth after antibiotic washout. 42 In this work, a decreased intracellular ATP level was observed as shown in Figure 7, indicating a remarkable decrease in cell viability caused by osthole.…”

“…It was reported that ATP fluctuations during antibiotic treatment could provide insight into drug efficacy. 41 Consequently, intracellular and extracellular ATP levels of L. monocytogenes were determined as shown in Figure 7. It can be seen that the intracellular ATP levels decreased significantly after treatment with osthole for 2 h. With the increased concentration of the osthole, intracellular ATP levels declined in a dose-dependent manner.…”