Single-dose local administration of parathyroid hormone (1–34, PTH) with β-tricalcium phosphate/collagen (β-TCP/COL) enhances bone defect healing in ovariectomized rats

Abstract

Parathyroid hormone (1-34, PTH) combined β-tricalcium phosphate (β-TCP) achieves stable bone regeneration without cell transplantation in previous studies. Recently, with the development of tissue engineering slow release technology, PTH used locally to promote bone defect healing become possible. This study by virtue of collagen with a combination of drugs and has a slow release properties, and investigated bone regeneration by β-TCP/collagen (β-TCP/COL) with the single local administration of PTH. After the creation of a rodent critical-sized femoral metaphyseal bone defect, β-TCP/COL was prepared by mixing sieved granules of β-TCP and atelocollagen for medical use, then β-TCP/COL with dripped PTH solution (1.0 µg) was implanted into the defect of OVX rats until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that single-dose local administration of PTH combined local usage of β-TCP/COL can increase the healing of defects in OVX rats. Furthermore, treatments with single-dose local administration of PTH and β-TCP/COL showed a stronger effect on accelerating the local bone formation than β-TCP/COL used alone. The results from our study demonstrate that combination of single-dose local administration of PTH and β-TCP/COL had an additive effect on local bone formation in osteoporosis rats.

Effects of strontium-modified calcium phosphate cement combined with bone morphogenetic protein-2 on osteoporotic bone defects healing in rats

“…The bone formations in the scaffolds were observed using a microscope and a digital camera (Leica Microsystems) as previously described. 25,26…”

Effect of alendronate on the femoral metaphyseal defect under carbamazepine in ovariectomized rats

“…A small DC drill was used under 0.9% sodium chloride to create a cylindrical defect with a diameter of 1.5 mm and a penetration length of about 4 mm. The femoral bony deficit is located slightly below the distal epiphyseal growth plate as described earlier [35] (Fig. 2).…”

Silibinin Promotes Bone Regeneration Through HIF-1α/VEGF and Notch Signaling Pathway in Ovariectomized Rats

“…So far, studies have con rmed that osteoporotic bones undergo a prolonged and impaired healing process in clinical postmenopausal osteoporotic women and experimental estrogen de ciency animal model [27,23]. Despite substantial advances in the eld of bone repair, strengthening the ability of bone formation remains a major challenge that has held back the clinical use of arti cial bone biomaterials.…”

“…12 weeks after sham operation(Sham) and bilateral ovariectomy(OVX), all rats were randomly divided into 3 groups: Sham group (Sham), OVX rats group (OVX) and OVX rats + silybin treatment group (SIL+OVX). Next, the rats of three groups established an intramedullary defect in the distal femur (1.5 mm in diameter×4mm in depth) using a 1.5 mm diameter clinical drill bits(Stryker Corporation, US) as described previously [22,23]. The rats were classi ed to SIL+OVX were treated with silibinin intraperitoneally with a dose of 50mg/kg (Sigma-Aldrich) [24].…”