Single-Dose Immunogenic DNA Vaccines Coding for Live-Attenuated Alpha- and Flaviviruses

Pushko, Peter; Lukashevich, Igor S.; Johnson, Dylan M.; Tretyakova, Irina

doi:10.3390/v16030428

Cited by 1 publication

(1 citation statement)

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“…Nevertheless, the main current application of DNA-launched in vivo infection is the development of new strategy to deliver LAV. By combining the ease of preparation, the genetic and thermal stability of traditional DNA vaccines with the high immunogenicity of conventional LAV, DNA-launched LAV could be a strategy for improving the biosafety and distribution of existing LAV [ 23 ]. Several studies have shown that DNA coding for live attenuated viral strains of several arboviruses, such as yellow fever virus and Venezuelan equine encephalitis virus, can induce a protective immune response in mice [ 24–36 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo rescue of arboviruses directly from subgenomic DNA fragments

Cochin,

Driouich,

Moureau

et al. 2024

Emerging Microbes & Infections

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Reverse genetic systems are mainly used to rescue recombinant viral strains in cell culture. These tools have also been used to generate, by inoculating infectious clones, viral strains directly in living animals. We previously developed the “Infectious Subgenomic Amplicons” (ISA) method, which enables the rescue of single-stranded positive sense RNA viruses in vitro by transfecting overlapping subgenomic DNA fragments. Here, we provide proof-of-concept for direct in vivo generation of infectious particles following the inoculation of subgenomic amplicons. First, we rescued a strain of tick-borne encephalitis virus in mice to transpose the ISA method in vivo . Subgenomic DNA fragments were amplified using a 3-fragment reverse genetics system and inoculated intramuscularly. Almost all animals were infected when quantities of DNA inoculated were at least 20 µg. We then optimized our procedure in order to increase the animal infection rate. This was achieved by adding an electroporation step and/or using a simplified 2- fragment reverse genetics system. Under optimal conditions, a large majority of animals were infected with doses of 20 ng of DNA. Finally, we demonstrated the versatility of this method by applying it to Japanese encephalitis and Chikungunya viruses. This method provides an efficient strategy for in vivo rescue of arboviruses. Furthermore, in the context of the development of DNA-launched live attenuated vaccines, this new approach may facilitate the generation of attenuated strains in vivo . It also enables to deliver a substance free of any vector DNA, which seems to be an important criterion for the development of human vaccines.

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Section: Discussionmentioning

confidence: 99%