Single DNA Origami Detection by Nanoimpact Electrochemistry

Abstract

DNA has emerged as the material of choice for producing supramolecular building blocks of arbitrary geometry from the 'bottom up'. Characterisation of these structures via electron or atomic force microscopy usually requires their surface immobilisation. In this work, we developed a nanoimpact electrochemistry platform to detect DNA self-assembled origami structures in solution, using the intercalator methylene blue as a redox probe. Here, we report the electrochemical detection of single DNA origami collisions at Pt microelectrodes. Our work paves the way towards the characterisation of DNA nanostructures in solution via nanoimpact electrochemistry.

Twisted DNA Origami-Based Chiral Monolayers for Spin Filtering

“…Introducing a right-handed tertiary chiral structure enhanced the CISS efficiency, while the left-handed structure had the opposite effect. This work, along with previous applications of DNA origami structures for electrochemical detection, − underscores the potential of DNA origami as a precise electronic manipulating device, offering valuable insights for developing novel electrochemical and electronic devices based on DNA origami structures.…”

CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

“…Electrochemistry has been a long-standing framework for elucidating the movement of electrons resulting from chemical reactions. Leveraging its attributes of rapid signal readout, cost-effective transduction element, and straightforward sensing platform, electrochemistry-based biosensing platforms have experienced significant advancements and widespread development. − Nowadays, most CRISPR-based systems rely on the trans -cleavage activity of Cas proteins combined with fluorescent, bioluminescent, or colorimetric reporters for readouts. ,, While these methods are accurate, rapid, and robust, the output signals they can generate are relatively weak, which limits their sensitivity. To address this limitation, considering the low concentration of target nucleic acids in the sample and the contamination caused by amplification, it is possible to combine the CRISPR system and electrochemical-based readout methods to develop a time-saving, high-sensitivity, and amplification-free biosensor for the detection of infectious diseases.…”

Electrode Nanopatterning for Bioelectroanalysis and Bioelectrocatalysis

“…Furthermore, the tetrahedron allowed tuning the position of cytochrome c relative to the electrode. Regarding DNA origamis, nanoimpact electrochemistry was developed to investigate their properties thanks to the intercalation of methylene blue 113 , opening the way to electrochemical nanosensors. miRNA-21 detection was achieved thanks to indirect measurement by intercalated methylene blue (MB) redox probe of the hybridization with ssDNA decorating a cross-shaped DNA origami 114 (Fig.…”