Single detection of human bocavirus 1 with a high viral load in severe respiratory tract infections in previously healthy children

Zhou, Lili; Zheng, Shouyan; Xiao, Qiuyan; Luo, Ren; Xie, Xiaohong; Luo, Jinyong; Wang, Lijia; Huang, Aiping; Liu, Wei; Liu, Enmei

doi:10.1186/1471-2334-14-424

Cited by 43 publications

(60 citation statements)

References 32 publications

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“…Si bien la mayoría de los autores reportó tasas superiores del virus en los meses de otoño e invierno [13][14][15]17,22 , estudios realizados en China 7,19 muestran una mayor frecuencia de HBoV en los meses de primavera y verano. Zhou y cols., realizaron un estudio durante tres años y hallaron tasas más elevadas en los meses de verano 19 .…”

Section: Discussionunclassified

“…El más frecuente fue VRS seguido de parainfluenza 3; también se detectaron ADV, MPVh e influenza A. El número de co-detecciones hallado en nuestro trabajo, si bien es alto, es inferior al reportado por otros autores, cuyos porcentajes oscilaron entre 30 y 76% 7,13,14,19,22 . Por este motivo, se debate si el virus causa infección respiratoria o es sólo un espectador de otras infecciones.…”

Section: Artículo Originalunclassified

“…Por este motivo, se debate si el virus causa infección respiratoria o es sólo un espectador de otras infecciones. No se encontró relación entre las coinfecciones y la aparición de enfermedad grave, al igual que en otras publicaciones 7,10,19 , en las que se describió que no existían diferencias significativas en la expresión clínica de los pacientes según estuvieran co-infectados o HBoV fuera el único agente detectado, en lo que respecta a signos y síntomas ni en el desarrollo de enfermedad grave.…”

Section: Artículo Originalunclassified

“…HBoV fue propuesto como causante de IRA, principalmente en lactantes 6,4 y se lo detectó en tasas variables, desde 0,9 a 33%, asociado a cuadro del tracto respiratorio superior, bronquiolitis, neumonía y exacerbación del asma 7,[13][14][15][16][17][18][19][20][21][22][23][24][25] . Sin embargo, HBoV tiene una elevada prevalencia en co-detecciones con otros patógenos 7,10,13,14,19,20,22 y se detectó en muestras de individuos asintomáticos 22,23,26 por lo cual aún debe ser confirmado su rol como patógeno.…”

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“…Sin embargo, HBoV tiene una elevada prevalencia en co-detecciones con otros patógenos 7,10,13,14,19,20,22 y se detectó en muestras de individuos asintomáticos 22,23,26 por lo cual aún debe ser confirmado su rol como patógeno.…”

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Detección de bocavirus humano en la población infantil de Tucumán y Santa Fe, Argentina

Vera-Garate

Rudi

Gómez

et al. 2016

Rev. chil. infectol.

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Section: Discussionunclassified

Section: Artículo Originalunclassified

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Detección de bocavirus humano en la población infantil de Tucumán y Santa Fe, Argentina

Vera-Garate

Rudi

Gómez

et al. 2016

Rev. chil. infectol.

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Development of RPA‐Cas12a‐fluorescence assay for rapid and reliable detection of human bocavirus 1

Qian

Wang

et al. 2023

Anim Models and Exp Med

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Human bocavirus (HBoV) 1 is considered an important pathogen that mainly affects infants aged 6–24 months, but preventing viral transmission in resource‐limited regions through rapid and affordable on‐site diagnosis of individuals with early infection of HBoV1 remains somewhat challenging. Herein, we present a novel faster, lower cost, reliable method for the detection of HBoV1, which integrates a recombinase polymerase amplification (RPA) assay with the CRISPR/Cas12a system, designated the RPA‐Cas12a‐fluorescence assay. The RPA‐Cas12a‐fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37°C without the need for sophisticated instruments. The method also demonstrates excellent specificity without cross‐reactivity to non‐target pathogens. Furthermore, the method was appraised using 28 clinical samples, and displayed high accuracy with positive and negative predictive agreement of 90.9% and 100%, respectively. Therefore, our proposed rapid and sensitive HBoV1 detection method, the RPA‐Cas12a‐fluorescence assay, shows promising potential for early on‐site diagnosis of HBoV1 infection in the fields of public health and health care. The established RPA‐Cas12a‐fluorescence assay is rapid and reliable method for human bocavirus 1 detection. The RPA‐Cas12a‐fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

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Atypical presentation of human bocavirus: Severe respiratory tract infection complicated with encephalopathy

Aktürk

Şık

Salman

et al. 2015

Journal of Medical Virology

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Human bocavirus (HBOV) has been reported as a worldwide distributed respiratory pathogen. It has also been associated with encephalitis recently by detection of the virus in cerebrospinal fluid (CSF) of patients presented with encephalitis. This retrospective study aimed to present clinical features of HBOV infections in children with respiratory symptoms and describe unexplained encephalopathy in a subgroup of these patients. Results of 1,143 pediatric nasal samples from mid-December 2013 to July 2014 were reviewed for detection of HBOV. A multiplex real time polymerase chain reaction assay was used for viral detection. Medical records of the patients were retrospectively analyzed. HBOV was detected in 30 patients (2.6%). Median age was 14 months (5-80). Clinical diagnoses were upper respiratory tract infection (n = 10), bronchopneumonia (n = 9), acute bronchiolitis (n = 5), pneumonia (n = 4), acute bronchitis (n = 1), and asthma execarbation (n = 1). Hospitalization was required in 16 (53.3%) patients and 10 (62.5%) of them admitted to pediatric intensive care unit (PICU). Noninvasive mechanical ventilation modalities was applied to four patients and mechanical ventilation to four patients. Intractable seizures developed in four patients while mechanically ventilated on the 2nd-3rd days of PICU admission. No specific reason for encephalopathy was found after a thorough investigation. No mortality was observed, but two patients were discharged with neurological sequela. HBOV may lead to respiratory infections in a wide spectrum of severity. This report indicates its potential to cause severe respiratory infections requiring PICU admission and highlights possible clinical association of HBOV and encephalopathy, which developed during severe respiratory infection.

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Single detection of human bocavirus 1 with a high viral load in severe respiratory tract infections in previously healthy children

Cited by 43 publications

References 32 publications

Detección de bocavirus humano en la población infantil de Tucumán y Santa Fe, Argentina

Detección de bocavirus humano en la población infantil de Tucumán y Santa Fe, Argentina

Development of RPA‐Cas12a‐fluorescence assay for rapid and reliable detection of human bocavirus 1

Atypical presentation of human bocavirus: Severe respiratory tract infection complicated with encephalopathy

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