The copper foil is taped to the side wall of a petri dish. Then warm LB agar liquid (10 g agar powder in 1 L medium, autoclaved) is poured into the dish and let consolidate to form agar plate. 50 µL of 1 × 10 6 CFU mL −1 S. aureus is added at the center of the agar plate and spread into a rectangular area with a cell spreader. The dish is moved under the plasma plume in a letter-writing route. Then the plates are incubated at 37 °C for 18 h after plasma treatment.