Single-chain Fv antibody with specificity for Listeria monocytogenes

Paoli, George C.; Chen, Chin-Yi; Brewster, Jeffrey D.

doi:10.1016/j.jim.2004.04.001

Cited by 42 publications

(34 citation statements)

References 30 publications

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“…The phage, Lm P4:A8, expressing the scFv antibody was isolated previously (Paoli et al, 2004) from the Griffin.1 library (Griffiths et al, 1994). The phage host strain E. coli TG1 and the library described above were both a gift of the Centre for Protein Engineering (Cambridge, UK).…”

Section: Phage Antibodymentioning

confidence: 99%

“…The phage host strain E. coli TG1 and the library described above were both a gift of the Centre for Protein Engineering (Cambridge, UK). Phage Lm P4:A8 was propagated using E. coli TG1 as described previously (Paoli et al, 2004). The concentration of phage used in this investigation was 5 × 10 10 transfecting units (TU)/ml.…”

Section: Phage Antibodymentioning

confidence: 99%

“…High cost of production, difficulty in reproducing stocks with similar target specificities, loss of functionality when exposed to extremes of the environment, and failure of in vivo systems to produce antibodies selective for important pathogens are some of the problems that are related to the use of antibodies in biosensor applications. To overcome these limitations, phage display technology has been used to develop single-chain antibodies for some pathogens (Benhar et al, 2001;Paoli et al, 2004). The filamentous bacteriophages used as probes in this study are bacterial viruses that possess a surface displayed single-chain antibody (scFv).…”

Section: Introductionmentioning

confidence: 99%

“…The scFv is expressed as a fusion to the minor coat protein, pIII, which is present in five copies on the bacteriophage surface. The phage probe used in this study, Lm P4:A8, was selected from a naive scFv phage display library for its specific interaction with L. monocytogenes (Paoli et al, 2004). The scFv expressed on the surface of phage Lm P4:A8 interacts with ActA (Paoli and Brewster, 2007), an L. monocytogenes protein virulence factor that is expressed on the bacterial cell surface and functions in host cell-to-cell movement via actin polymerization.…”

Section: Introductionmentioning

confidence: 99%

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SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

Nanduri

Bhunia

et al. 2007

Biosensors and Bioelectronics

133

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Section: Phage Antibodymentioning

confidence: 99%

Section: Phage Antibodymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

Nanduri

Bhunia

et al. 2007

Biosensors and Bioelectronics

133

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“…10 L. monocytogenes 4b specific monoclonal antibodies showed cross reaction with serotype 4d and 4e and L. innocua strains. 20 Paoli et al 21 developed a singlechain Fv antibody to L. monocytogenes that reacted with no other Listeria species, but failed to react with several serovars of L. monocytogenes. A polyclonal antibody against internalin B was also reported, and this antibody was used in a surface plasmon resonance sensor for detection of L. monocytogenes.…”

Section: Lathrop Et Almentioning

confidence: 99%

Pathogen-specific antigen target for production of antibodies produced by comparative genomics

Lathrop¹,

Bailey²,

Kim³

et al. 2014

ANTI

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Listeria monocytogenes continues to be a major public health risk and there is a need for improved rapid detection methods. New and highly specific L. monocytogenes antibodies are needed to advance current detection and meet the needs of industry. This research compared the L. monocytogenes genome with that of L. innocua (a nonpathogenic species of Listeria) and identified nine surface proteins specific to L. monocytogenes. Protein sequences were collected from the database and properties such as hydropathy profile and transmembrane topology were analyzed using TMpred software. Nine peptide sequences were chosen, and synthetic peptides were made and administered to rabbits for antibody production. All nine antibodies were screened against a panel of L. monocytogenes, nonpathogenic Listeria, and nonListeria bacteria. Two of the nine antibodies, ie, the Lm404 and LmC639 polyclonal antibodies, showed a specific reaction to L. monocytogenes internalin B and actin polymerization protein, respectively, and were characterized further by enzyme-linked immunosorbent assay, Western blot, and transmission electron microscopy. In Western blot, both antibodies reacted with the targeted protein and did not cross-react with other Listeria spp. The Lm404 polyclonal antibody showed a high reaction with the panel of 41 L. monocytogenes strains while the LmC639 polyclonal antibody showed a weak reaction. Both the Lm404 and LmC639 polyclonal antibodies showed potential for use in immunoassays for specific detection of L. monocytogenes. This study further indicates that comparative genomics could be used to select pathogen-specific antigen for antibody production.

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Antibodies and Immunoassays for Detection of Bacterial Pathogens

Banada

Bhunia

Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems

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Single-chain Fv antibody with specificity for Listeria monocytogenes

Cited by 42 publications

References 30 publications

SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

Pathogen-specific antigen target for production of antibodies produced by comparative genomics

Antibodies and Immunoassays for Detection of Bacterial Pathogens

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