Single chain antibody fragment with serine protease inhibitory property capable of neutralizing toxicity of Trimeresurus mucrosquamatus venom

Lee, Yu Ching; Chen, Wang Chuan; Liang, Meng Huei; Lee, Chi Hsin; Tsai, Keng‐Chang; Chiang, Jen-Ron; Chiang, Liao-Chun; Chen, Chi Ching; Chang, Chang Yu; Lee, Ching Hsiao; Leu, Sy Jye; Yang, Yi Yuan

doi:10.1016/j.bbrc.2015.02.169

Cited by 4 publications

(2 citation statements)

References 21 publications

(23 reference statements)

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“…Our previous study indicated that the hemolytic activity of SVSP in TM venom proteins was significantly inhibited by scFv antibodies [51]. However, anti-TS scFv antibodies (TSS1, TSL7, and TSL8) obtained in this study exhibited partial inhibitory activities on SVSPs (Additional file 2 and Additional file 3).…”

Section: Discussionmentioning

confidence: 74%

“…In this study, we established two scFv antibody libraries with 2.4 × 10 7 and 6.8 × 10 7 phage clones from hens administered with TS venom proteins. Our previous studies have shown that the size of libraries was sufficient for the generation of specific antibodies [43,44,51,52,53,54]. Despite generating from naïve antibody libraries, which avoid animal immunization, it requires sizable libraries and extensive biopanning steps for the generation of specific antibodies [55].…”

Section: Discussionmentioning

confidence: 99%

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Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

Lee

Liu

Leu

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: The venom of bamboo vipers (Trimeresurus stejnegeri-TS), commonly found in Taiwan, contains deadly hemotoxins that cause severe envenomation. Equinederived antivenom is a specific treatment against snakebites, but its production costs are high and there are some inevitable side effects. The aim of the present work is to help in the development of an affordable and more endurable therapeutic strategy for snakebites. Methods: T. stejnegeri venom proteins were inactivated by glutaraldehyde in order to immunize hens for polyclonal immunoglobulin (IgY) antibodies production. After IgY binding assays, two antibody libraries were constructed expressing single-chain variable fragment (scFv) antibodies joined by the short or long linker for use in phage display antibody technology. Four rounds of biopanning were carried out. The selected scFv antibodies were then further tested for their binding activities and neutralization assays to TS proteins. Results: Purified IgY from egg yolk showed the specific binding ability to TS proteins. The dimensions of these two libraries contain 2.4 × 10 7 and 6.8 × 10 7 antibody clones, respectively. An increase in the titers of eluted phage indicated anti-TS clones remarkably enriched after 2 nd panning. The analysis based on the nucleotide sequences of selected scFv clones indicated that seven groups of short linkers and four groups of long linkers were identified. The recombinant scFvs showed significant reactivity to TS venom proteins and a cross-reaction to Trimeresurus mucrosquamatus venom proteins. In in vivo studies, the data demonstrated that anti-TS IgY provided 100% protective effects while combined scFvs augmented partial survival time of mice injected with a lethal amount of TS proteins. Conclusion: Chickens were excellent hosts for the production of neutralization antibodies at low cost. Phage display technology is available for generation of monoclonal antibodies against snake venom proteins. These antibodies could be applied in the development of diagnostic kits or as an alternative for snakebite envenomation treatment in the near future.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

Lee

Liu

Leu

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

Self Cite

View full text Add to dashboard Cite

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Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display

Kühn

Fühner

Unkauf

et al. 2016

Proteomics Clinical Apps

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Antibodies are valuable molecules for the diagnostic and treatment of diseases caused by pathogens and toxins. Traditionally, these antibodies are generated by hybridoma technology. An alternative to hybridoma technology is the use of antibody phage display to generate recombinant antibodies. This in vitro technology circumvents the limitations of the immune system and allows-in theory-the generation of antibodies against all conceivable molecules. Phage display technology enables obtaining human antibodies from naïve antibody gene libraries when either patients are not available or immunization is not ethically feasible. On the other hand, if patients or immunized/infected animals are available, it is common to construct immune phage display libraries to select in vivo affinity-matured antibodies. Because the phage packaged DNA sequence encoding the antibodies is directly available, the antibodies can be smoothly engineered according to the requirements of the final application. In this review, an overview of phage display derived recombinant antibodies against bacterial, viral, and eukaryotic pathogens as well as toxins for diagnostics and therapy is given.

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Generation and characterization of avian-derived anti-human CD19 single chain fragment antibodies

Tsai

Chiang

et al. 2018

Animal Biotechnology

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Single chain antibody fragment with serine protease inhibitory property capable of neutralizing toxicity of Trimeresurus mucrosquamatus venom

Cited by 4 publications

References 21 publications

Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display

Generation and characterization of avian-derived anti-human CD19 single chain fragment antibodies

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