Single chain anti-c-Met antibody conjugated nanoparticles for in vivo tumor-targeted imaging and drug delivery

Lu, Ruei‐Min; Chang, Yu‐Ling; Chen, Minshan; Wu, Han-Chung

doi:10.1016/j.biomaterials.2010.12.061

Cited by 112 publications

(68 citation statements)

References 39 publications

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“…Significant tumor reduction with tumor vasculature disruption was spotted in vivo for the targeted system. [121] scFvs have been extensively investigated as new targeting ligands on nanoparticles using many of the chemistries described in the previous sections of this report. This includes amide formation, [122] Michael addition through site-specific addition to the an engineered free C-terminal sulfydryl group on the scFv [5b] and through the biotin-avidin approach where the scFc was biotinylated and attached to a polymer-avidin conjugate.…”

Section: Synthesis Of Antibody Fragment-nanomaterials Conjugatesmentioning

confidence: 99%

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Sivaram

Wardiana

Howard

et al. 2017

Adv Healthcare Materials

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Targeted nanomedicines have significantly changed the way new therapeutics are designed to treat disease. Central to successful therapeutics is the ability to control the dynamics of protein–nanomaterial interactions to enhance the therapeutic effect of the nanomedicine. The aim of this review is to illustrate the diversity and versatility of the conjugation approaches involved in the synthesis of antibody–nanoparticle conjugates, and highlight significant new advances in the field of bioconjugation. Such nanomedicines have found utility as both advanced therapeutic agents, as well as more complex imaging contrast agents that can provide both anatomical and functional information of diseased tissue. While such conjugates show significant promise as next generation targeted nanomedicines, it is recognized that there are in fact no clinically approved targeted therapeutics on the market. This fact is reflected upon within this review, and attempts are made to draw some reasoning from the complexities associated with the bioconjugation chemistry approaches that are typically utilized. Present trends, as well as future directions of next generation targeted nanomedicines are also discussed.

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Section: Synthesis Of Antibody Fragment-nanomaterials Conjugatesmentioning

confidence: 99%

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Sivaram

Wardiana

Howard

et al. 2017

Adv Healthcare Materials

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“…22,23 Two methods have been successfully employed to synthesize Mal-PEG-DSPE (Figure 2). 24 In the first method, amino-PEG-DSPE reacted with N-succinimidyl-3-(N-maleimido)-propionate in CH 2 Cl 2 , DMF, and triethylamine to form Mal-PEG-DSPE.…”

Section: -21mentioning

confidence: 99%

Application of poly(ethylene glycol)– distearoylphosphatidylethanolamine (PEG-DSPE) block copolymers and their derivatives as nanomaterials in drug delivery

Wang

Xiao²,

Zeng³

et al. 2012

IJN

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Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) block copolymers are biocompatible and amphiphilic polymers that can be widely utilized in the preparation of liposomes, polymeric nanoparticles, polymer hybrid nanoparticles, solid lipid nanoparticles, lipid-polymer hybrid nanoparticles, and microemulsions. Particularly, the terminal groups of PEG can be activated and linked to various targeting ligands, which can prolong the circulation time, improve the drug bioavailability, reduce undesirable side effects, and especially target specific cells, tissues, and even the intracellular localization in organelles. This review herein aims to describe recent developments in drug carriers exploiting PEG-DSPE block copolymers and their derivatives, and the incorporation of different ligands to the end groups of PEG-DSPE to target delivery, focusing on their modification approaches, advantages, applications, and the probable associated drawbacks.

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“…ScFv can be conjugated to different types of molecules, such as radioisotopes and toxins, or expressed as an intracellular antibody (intrabody) in a specific intracellular compartment, where it can interfere with the function of the targeted antigen at the level of a specific domain [13] . Human single chain antibody fragments offer the advantage of being expressed as a single polypeptide, and their small size means that they can serve as a highly safe, selective and effective diagnostic and therapeutical tool [10,[14][15][16][17][18][19][20] . The scFv on target molecules within cells or intrabodies provides a useful tool for research as well as for control of diseases such as human immunodeficiency virus type 1 infection and other diseases [13,15,16,21] .…”

Section: Introductionmentioning

confidence: 99%

Anti-CHMP5 single chain variable fragment antibody retrovirus infection induces programmed cell death of AML leukemic cells in vitro

et al. 2012

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Aim: Over-expressed CHMP5 was found to act as oncogene that probably participated in leukemogenesis. In this study, we constructed the CHMP5 single chain variable fragment antibody (CHMP5-scFv) retrovirus and studied the changes of programmed cell death (PCD) of AML leukemic cells after infection by the retrovirus. Methods: The anti-CHMP5 KC14 hybridoma cell line was constructed to generate monoclonal antibody of CHMP5. The protein expression of CHMP5 was studied using immunofluorescence analysis. pMIG-CHMP5 scFv antibody expressible retroviral vector was constructed to prepare CHMP5-scFv retrovirus. AML leukemic U937 cells were infected with the retrovirus, and programmed cell death was studied using confocal microscope, FCM and Western blot. Results: We obtained a monoclonal antibody of CHMP5, and found the expression of CHMP5 was up-regulated in the leukemic cells. After U937 cells were infected with CHMP5-scFv retrovirus, CHMP5 protein was neutralized. Moreover, the infection resulted in a significant increase in apoptosis and necrosis of U937 cells. In U937 cells infected with CHMP5-scFv retrovirus, apoptosis-inducing factor (AIF)-mediated caspase-independent necrotic PCD was activated, but autophagic programmed cell death was not observed. Neither the intrinsic nor extrinsic apoptotic PCD pathway was activated. The granzyme B/perforin-mediated caspase-dependent apoptotic PCD pathway was not activated. Conclusion: CHMP5-scFv retrovirus can neutralize the abnormally high levels of the CHMP5 protein in the cytosol of AML leukemic U937 cells, thereby inducing the programmed cell death of the leukemic cells via AIF-mediated caspase-independent necrosis and apoptosis.

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Single chain anti-c-Met antibody conjugated nanoparticles for in vivo tumor-targeted imaging and drug delivery

Cited by 112 publications

References 39 publications

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Application of poly(ethylene glycol)– distearoylphosphatidylethanolamine (PEG-DSPE) block copolymers and their derivatives as nanomaterials in drug delivery

Anti-CHMP5 single chain variable fragment antibody retrovirus infection induces programmed cell death of AML leukemic cells in vitro

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Single chain anti-c-Met antibody conjugated nanoparticles for in vivo tumor-targeted imaging and drug delivery

Cited by 112 publications

References 39 publications

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Application of poly(ethylene glycol)&ndash; distearoylphosphatidylethanolamine (PEG-DSPE) block copolymers and their derivatives as nanomaterials in drug delivery

Anti-CHMP5 single chain variable fragment antibody retrovirus infection induces programmed cell death of AML leukemic cells in vitro

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Application of poly(ethylene glycol)– distearoylphosphatidylethanolamine (PEG-DSPE) block copolymers and their derivatives as nanomaterials in drug delivery