Abstract:Several methods for generating human skin equivalent (HSE) organoid cultures are regularly used to study skin biology and test pharmaceuticals, however few studies have thoroughly characterized these systems. To fill this gap, we used single cell-RNA sequencing to compare the cellular states of in vitro HSEs generated from distinct culture methods, HSEs xenografted onto mice, and in vivo epidermis. By combining differential gene expression, pseudotime analyses, splicing kinetics, and spatial localization, we r… Show more
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