Single-cell transcriptome profiling reveals the mechanism of abnormal proliferation of epithelial cells in congenital cystic adenomatoid malformation

Zhang, Shouhua; Ye, Chunjing; Xiao, Jianghong; Yang, Jiale; C, Zhu; Xiao, Yao; Ye, Ming; Chen, Qiang

doi:10.1016/j.yexcr.2020.112299

Cited by 4 publications

(4 citation statements)

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“…[ 79 ] TGFB1 secreted by MACs binds to TGFBR3, which is a regulating growth factor during repairing, [ 80 ] promoting cell migration and epithelial cell proliferation together with FAM3C. [ 81 ] We then analyzed the CellChat between IFECs (Figure 8B) and found that the expressions of receptor ligands were very similar, suggesting that they may exhibit similar effects. In epidermis, NECTIN is expressed at cell‐cell junctions mainly in the suprabasal layer.…”

Section: Resultsmentioning

confidence: 99%

“…[84] LAMP1 and FAM3C can promote epithelial cell proliferation. [81] For endothelial cells and M1 MACs (Figure 8C), CXCL9 secreted by endothelial cells is a pro-inflammatory chemokine that triggers immune cell migration and rapid relocation to boost antibody secretion at the infected site. [85] MACs AREG receptors bind to ICAM1 of endothelial cells and regulate stem cell pluripotency of endothelial cells.…”

Section: Therapeutic Mechanisms Of Sa For Wound Healing In Ratsmentioning

confidence: 99%

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Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Zhang

Song

et al. 2023

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Complete skin reconstruction is a hierarchical, physiological assembly process involving healing of the epidermis, dermis, vasculature, nerves, and cutaneous appendages. To date, few works have reported complete skin regeneration, particularly lacking vascular structures and hair follicles after full skin defects. In this study, a hydrogel derived from the skin secretion of Andrias davidianus (SSAD) that features adhesiveness was used as a bioactive scaffold to load micronized amnion (MA). The SSAD hydrogel was found to promote the migration and proliferation of amnion stem cells and human keratinocytes, as well as inhibit their apoptosis in vitro. In a rat full‐skin defect model, the regeneration of skin appendages was observed at the wound area, achieving scarless healing. Transcriptome analyses further validated that SSAD could positively regulate cell migration, proliferation, and differentiation. These functions might be attributed to the abundant growth factors present in the SSAD. Synergized by the delivery of MA, SSAD loaded with the MA could achieve a significantly better skin regeneration effect than SSAD or MA used alone, providing a simple yet highly effective means to obtain complete, scarless skin regeneration, suggesting favorable potential for clinical translation.

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Section: Resultsmentioning

confidence: 99%

Section: Therapeutic Mechanisms Of Sa For Wound Healing In Ratsmentioning

confidence: 99%

Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Zhang

Song

et al. 2023

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“…S3 ). We further investigated the expression of SPOCK2 in epithelial subtypes of ciliated cells, alveolar type 1 (AT1), alveolar type 2 (AT2), club cells, and basal cells based on the scRNA-seq data [ 27 ]. No expression was detected in basal cells, but a relatively higher expression of SPOCK2 was found in AT1 cells than in other cell types.…”

Section: Resultsmentioning

confidence: 99%

“…While deregulated SHH signaling was thought to be the pathogenesis of lung cystic abnormalities [ 32 ]. Of note, pseudotime trajectory analysis from scRNA-seq revealed that epithelial subtypes of ciliated cells, AT1 and AT2 originated from the club and basal cells and were at the end of pseudotime [ 27 ]. Our CPAM tissues and their paired simply controls were with the postnatal collection.…”

Section: Discussionmentioning

confidence: 99%

Integrated bulk and single-cell RNA-sequencing reveals SPOCK2 as a novel biomarker gene in the development of congenital pulmonary airway malformation

Tan

Chen

et al. 2023

Respir Res

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Background Congenital pulmonary airway malformation (CPAM) is the most frequent pulmonary developmental malformation and the pathophysiology remains poorly understood. This study aimed to identify the characteristic gene expression patterns and the marker genes essential to CPAM. Methods Tissues from the cystic area displaying CPAM and the area of normal appearance were obtained during surgery. Bulk RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) were performed for integrating analysis. Iterative weighted gene correlation network analysis (iWGCNA) was used to identify specifically expressed genes to CPAM. Results In total, 2074 genes were significantly differentially expressed between the CPAM and control areas. Of these differentially expressed genes (DEGs), 1675 genes were up-regulated and 399 genes were down-regulated. Gene ontology analysis revealed these DEGs were specifically enriched in ciliated epithelium and involved in immune response. We also identified several CPAM-related modules by iWGCNA, among them, P15_I4_M3 module was the most influential module for distinguishing CPAMs from controls. By combining the analysis of the expression dataset from RNA-seq and scRNA-seq, SPOCK2, STX11, and ZNF331 were highlighted in CPAM. Conclusions Through our analysis of expression datasets from both scRNA-seq and bulk RNA-seq of tissues obtained from patients with CPAM, we identified the characteristic gene expression patterns associated with the condition. Our findings suggest that SPOCK2 could be a potential biomarker gene for the diagnosis and therapeutic target in the development of CPAM, whereas STX11 and ZNF331 might serve as prognostic markers for this condition. Further investigations with larger samples and function studies are necessary to confirm the involvement of these genes in CPAM.

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Congenital lung malformations: Dysregulated lung developmental processes and altered signaling pathways

Doktor¹,

Antounians²,

Lacher³

et al. 2022

Seminars in Pediatric Surgery

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Single-cell transcriptome profiling reveals the mechanism of abnormal proliferation of epithelial cells in congenital cystic adenomatoid malformation

Cited by 4 publications

References 32 publications

Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Integrated bulk and single-cell RNA-sequencing reveals SPOCK2 as a novel biomarker gene in the development of congenital pulmonary airway malformation

Congenital lung malformations: Dysregulated lung developmental processes and altered signaling pathways

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