Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells

Waki, Michihiko; Ide, Yoshimi; Ishizaki, Itsuko; Nagata, Yasuyuki; Nio, Masaki; Sugiyama, Eiji; Kurabe, Nobuya; Nicolaescu, D.; Yamazaki, Fumiyoshi; Hayasaka, Takahiro; Ikegami, Koji; Kondo, Takeshi; Shibata, Kiyoshi; Hiraide, Takanori; Taki, Yumiko; Ogura, Hironao; Shiiya, Norihiko; Sanada, N.; Setou, Mitsutoshi

doi:10.1016/j.biochi.2014.10.003

Cited by 26 publications

(20 citation statements)

References 23 publications

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“…They also demonstrated that the relative amount of PE was increased in cancerous tissues when compared to normal tissues. Recently, Waki et al () conducted TOF‐SIMS study to analyze PLs in breast cancer stem cells (CSCs) and non‐stem cancer cells (NSCCs), where PC(16:0/16:1) was less abundant and PC(16:0/16:0) was more abundant in CSCs compared to NSCCs. Very recently, Mao et al () investigated a novel air flow‐assisted ionization (AFAI) MSI study on tissue samples of breast invasive ductal carcinoma (IDC) and breast ductal carcinoma in situ (DCIS) during surgery of BC patients to identify lipid profiles.…”

Section: Ms‐based Analysis Of Pls In Various Cancers and Biomarker DImentioning

confidence: 99%

Phospholipids as cancer biomarkers: Mass spectrometry‐based analysis

Bandu

Mok

Kim

2016

Mass Spectrometry Reviews

145

114

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Lipids, particularly phospholipids (PLs), are key components of cellular membrane. PLs play important and diverse roles in cells such as chemical-energy storage, cellular signaling, cell membranes, and cell-cell interactions in tissues. All these cellular processes are pertinent to cells that undergo transformation, cancer progression, and metastasis. Thus, there is a strong possibility that some classes of PLs are expected to present in cancer cells and tissues in cellular physiology. The mass spectrometric soft-ionization techniques, electrospray ionization (ESI), and matrix-assisted laser desorption/ionization (MALDI) are well-established in the proteomics field, have been used for lipidomic analysis in cancer research. This review focused on the applications of mass spectrometry (MS) mainly on ESI-MS and MALDI-MS in the structural characterization, molecular composition and key roles of various PLs present in cancer cells, tissues, blood, and urine, and on their importance for cancer-related problems as well as challenges for development of novel PL-based biomarkers. The profiling of PLs helps to rationalize their functions in biological systems, and will also provide diagnostic information to elucidate mechanisms behind the control of cancer, diabetes, and neurodegenerative diseases. The investigation of cellular PLs with MS methods suggests new insights on various cancer diseases and clinical applications in the drug discovery and development of biomarkers for various PL-related different cancer diseases. PL profiling in tissues, cells and body fluids also reflect the general condition of the whole organism and can indicate the existence of cancer and other diseases. PL profiling with MS opens new prospects to assess alterations of PLs in cancer, screening specific biomarkers and provide a basis for the development of novel therapeutic strategies. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:107-138, 2018.

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Section: Ms‐based Analysis Of Pls In Various Cancers and Biomarker DImentioning

confidence: 99%

Phospholipids as cancer biomarkers: Mass spectrometry‐based analysis

Bandu

Mok

Kim

2016

Mass Spectrometry Reviews

145

114

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“…Schober et al was the first to develop single-cell MALDI-IMS analysis using the HeLa cell line [27]; however, no report has examined comparative lipid profiling by single-cell MALDI-IMS analysis. Several reports have been published on comparative analysis of single cells using secondary ion mass spectrometry (SIMS), and these reports have demonstrated fatty acid profiles in the single-cell level using clinical samples such as breast cancer stem cell and MM cell [28,29]. Liquid chromatography-MS was also used for analyzing the profile of lipids in a purified cell; however, this method requires extraction and bulk amounts of cells and misses specific information on rare target populations due to the heterogeneity of cells in tissues or groups [28,30].…”

Section: Discussionmentioning

confidence: 99%

Decreased level of phosphatidylcholine (16:0/20:4) in multiple myeloma cells compared to plasma cells: a single-cell MALDI–IMS approach

et al. 2015

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Lipid metabolic changes under diseased conditions, particularly in solid tumors, are attracting increased attention. However, in non-solid tumors, including most hematopoietic tumors, lipid analyses are scarce. Multiple myeloma (MM) is a plasma cell disorder arising from bone marrow, and the lipid status of MM cells has not been reported yet. In this study, we analyzed flow cytometry-sorted single MM cells and normal plasma cells (NPCs) using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), a two-dimensional label-free mass spectrometry technique for biomolecular analysis, to obtain specific lipid information. We isolated 1.31-5.77% of MM cells and 0.03-0.24% of NPCs using fluorescence-activated cell sorting (FACS). Analysis of purified cells using MALDI-IMS at the single-cell level revealed that the peak intensity and ion signals of phosphatidylcholine [PC (16:0/20:4) + H](+) at m/z 782.5 were significantly decreased in MM cells compared to NPCs. By examining particular cell populations rather than cell mixtures, our method can become a suitable tool for the analysis of rare cell populations at the single-cell level and advance the understanding of MM progression.

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“…Neither further differences in fatty acid composition nor specific membrane structures were observed. The study may give hints for cancer therapy and represents an example for the application of FACS and ToF-SIMS for imaging of cellular membrane components [ 55 ].…”

Section: Lipidomicsmentioning

confidence: 99%

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS): A New Tool for the Analysis of Toxicological Effects on Single Cell Level

Jungnickel

Laux

Luch

2016

Toxics

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Abstract:Single cell imaging mass spectrometry opens up a complete new perspective for strategies in toxicological risk assessment and drug discovery. In particular, time-of-flight secondary ion mass spectrometry (ToF-SIMS) with its high spatial and depth resolution is becoming part of the imaging mass spectrometry toolbox used for single cell analysis. Recent instrumentation advancements in combination with newly developed cluster ion guns allow 3-dimensional reconstruction of single cells together with a spatially resolved compound location and quantification on nanoscale depth level. The exact location and quantification of a single compound or even of a set of compounds is no longer restricted to the two dimensional space within single cells, but is available for voxels, a cube-sized 3-dimensional space, rather than pixels. The information gathered from one voxel is further analysed using multivariate statistical methodology like maximum autocorrelation factors to co-locate the compounds of interest within intracellular organelles like nucleus, mitochondria or golgi apparatus. Furthermore, the cell membrane may be resolved, including adhering compounds and potential changes of the lipid patterns. The generated information can be used further for a first evaluation of intracellular target specifity of new drug candidates or for the toxicological risk assessment of environmental chemicals and their intracellular metabolites. Additionally, single cell lipidomics and metabolomics enable for the first time an in-depth understanding of the activation or inhibition of cellular biosynthesis and signalling pathways.

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Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells

Cited by 26 publications

References 23 publications

Phospholipids as cancer biomarkers: Mass spectrometry‐based analysis

Phospholipids as cancer biomarkers: Mass spectrometry‐based analysis

Decreased level of phosphatidylcholine (16:0/20:4) in multiple myeloma cells compared to plasma cells: a single-cell MALDI–IMS approach

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS): A New Tool for the Analysis of Toxicological Effects on Single Cell Level

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