Single-cell temporal transcriptomics from tiny cytoplasmic biopsies

Horváth, Róbert

doi:10.1016/j.crmeth.2022.100319

Cited by 3 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Remarkably, this aspect remains unaddressed not only in our study but also in most studies related to cuproptosis. Real-time monitoring of cellular responses may be necessary during induced cuproptosis, and a recently developed sequencing technology called Live-seq could potentially provide valuable insights for future research in this area [ 79 ]. It is noteworthy that our study is constrained by its confinement to cell experiments and colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

The crosstalk role of CDKN2A between tumor progression and cuproptosis resistance in colorectal cancer

Cheng,

Yang,

et al. 2024

Aging

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

The crosstalk role of CDKN2A between tumor progression and cuproptosis resistance in colorectal cancer

Cheng,

Yang,

et al. 2024

Aging

View full text Add to dashboard Cite

“…Single-cell temporal transcriptomics from tiny FluidFM biopsies (LiveSeq) could address a broad range of basic biological questions. The method introduced recently by the groups of Vorholt and Deplancke transformed scRNA-seq from an endpoint to a temporal analysis workflow with the help of FluidFM 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic function and spring constant calibration of FluidFM micropipette cantilevers

Bonyár,

Nagy,

Gunstheimer

et al. 2024

Microsyst Nanoeng

Self Cite

View full text Add to dashboard Cite

Fluidic force microscopy (FluidFM) fuses the force sensitivity of atomic force microscopy with the manipulation capabilities of microfluidics by using microfabricated cantilevers with embedded fluidic channels. This innovation initiated new research and development directions in biology, biophysics, and material science. To acquire reliable and reproducible data, the calibration of the force sensor is crucial. Importantly, the hollow FluidFM cantilevers contain a row of parallel pillars inside a rectangular beam. The precise spring constant calibration of the internally structured cantilever is far from trivial, and existing methods generally assume simplifications that are not applicable to these special types of cantilevers. In addition, the Sader method, which is currently implemented by the FluidFM community, relies on the precise measurement of the quality factor, which renders the calibration of the spring constant sensitive to noise. In this study, the hydrodynamic function of these special types of hollow cantilevers was experimentally determined with different instruments. Based on the hydrodynamic function, a novel spring constant calibration method was adapted, which relied only on the two resonance frequencies of the cantilever, measured in air and in a liquid. Based on these results, our proposed method can be successfully used for the reliable, noise-free calibration of hollow FluidFM cantilevers.

show abstract

Artificial Breakthrough of Cell Membrane Barrier for Transmembrane Substance Exchange: A Review of Recent Progress

Wang,

Zhu,

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Cell membrane composed of lipid bilayer is a selectively permeable membrane that only allows specific molecules to pass through. While such selectivity is essential for the survival and function of cells, there exist instances when it is necessary to overcome the cell membrane barrier. Study on the artificial cell membrane barrier breakthrough strategies is of great significance for the development of drug delivery systems and the understanding of cellular behaviors. Herein, the advancements in the development of strategies for opening cell membrane barriers over the past decade are summarized. The main transmembrane mechanisms are elucidated and then divided into three categories, i.e., cell perforation via microinjection/external physical fields, cell endocytosis‐assisted construction of artificial transmembrane channels, and untethered micro/nanomachines. Next, the potential applications after opening the cell membrane are discussed, which mainly focus on the transmembrane cargo delivery into the cell and endogenous substance extraction out from the cell. Finally, this review outlines the current challenges that impede the realization of practical applications and presents an outlook of future opportunities to promote further development. Through overcoming the challenges, it is anticipated that artificial cell membrane breakthrough strategies will provide a revolutionized tool in the near future to advance the field of biomedicine and biotechnology.

show abstract

Single-cell temporal transcriptomics from tiny cytoplasmic biopsies

Cited by 3 publications

References 10 publications

The crosstalk role of CDKN2A between tumor progression and cuproptosis resistance in colorectal cancer

The crosstalk role of CDKN2A between tumor progression and cuproptosis resistance in colorectal cancer

Hydrodynamic function and spring constant calibration of FluidFM micropipette cantilevers

Artificial Breakthrough of Cell Membrane Barrier for Transmembrane Substance Exchange: A Review of Recent Progress

Contact Info

Product

Resources

About