Single-cell technologies and analyses in hematopoiesis and hematological malignancies

Campillo-Marcos, Ignacio; Álvarez-Errico, Damiana; Alandes, Regina A.; Mereu, Elisabetta; Esteller, Manel

doi:10.1016/j.exphem.2021.05.001

Cited by 11 publications

(7 citation statements)

References 153 publications

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“…In recent years, single-cell-sequencing approaches have revolutionized our understanding of normal and malignant hematopoiesis by enabling the precise characterization of the transitional cell states between hematopoietic hierarchy stages and their HSPC onset clonal origins that are dysregulated during hematologic malignancies [ 120 , 121 ]. Knowing the characteristics of normal cells should facilitate understanding their malignant counterparts and thus provide clues on how CH shifts the HSPC transcriptional landscape toward clonal expansion and lineage skewing.…”

Section: Embracing Complexity: New Tools To Study the Ch Bm Nichementioning

confidence: 99%

Clonal hematopoiesis and its impact on the aging osteo-hematopoietic niche

Winter,

Götze,

Hecker

et al. 2024

Leukemia

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Clonal hematopoiesis (CH) defines a premalignant state predominantly found in older persons that increases the risk of developing hematologic malignancies and age-related inflammatory diseases. However, the risk for malignant transformation or non-malignant disorders is variable and difficult to predict, and defining the clinical relevance of specific candidate driver mutations in individual carriers has proved to be challenging. In addition to the cell-intrinsic mechanisms, mutant cells rely on and alter cell-extrinsic factors from the bone marrow (BM) niche, which complicates the prediction of a mutant cell’s fate in a shifting pre-malignant microenvironment. Therefore, identifying the insidious and potentially broad impact of driver mutations on supportive niches and immune function in CH aims to understand the subtle differences that enable driver mutations to yield different clinical outcomes. Here, we review the changes in the aging BM niche and the emerging evidence supporting the concept that CH can progressively alter components of the local BM microenvironment. These alterations may have profound implications for the functionality of the osteo-hematopoietic niche and overall bone health, consequently fostering a conducive environment for the continued development and progression of CH. We also provide an overview of the latest technology developments to study the spatiotemporal dependencies in the CH BM niche, ideally in the context of longitudinal studies following CH over time. Finally, we discuss aspects of CH carrier management in clinical practice, based on work from our group and others.

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Section: Embracing Complexity: New Tools To Study the Ch Bm Nichementioning

confidence: 99%

Clonal hematopoiesis and its impact on the aging osteo-hematopoietic niche

Winter,

Götze,

Hecker

et al. 2024

Leukemia

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“…With the advent of single cell RNA-sequencing (scRNA-seq), the transcriptome has become the molecular level most exploited by single cell technologies. It has accelerated our understanding of cancer biology, enabling the characterization of the intratumoral heterogeneity and cellular architecture of several cancer types at unprecedented resolution [20,[30][31][32][33]. Additionally, there are emerging single cell DNA-sequencing technologies that allow us to profile, in an amplicon-based and targeted manner, recurrently mutated genes, providing the genotype of every cell by detecting single nucleotide variants (SNPs) and copy number variants (CNVs) [34].…”

Section: Single Cell Mono-omic Technologies To Explore Cancer Epigeno...mentioning

confidence: 99%

Single cell cancer epigenetics

2022

Self Cite

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“…Fortunately, the recent explosion in molecular biology techniques and computational approaches makes it possible to overcome the above problems. Artificial intelligence (AI)-based methods have been successfully applied to several tasks of single-cell omics, representing powerful and promising tools for biological discovery ( 123 ). The power of single-cell metabolomics can be improved by combining artificial intelligence-based algorithms.…”

Section: Challenges and Perspectives Of Single-cell Metabolomicsmentioning

confidence: 99%

Single-Cell Metabolomics in Hematopoiesis and Hematological Malignancies

Zuo

2022

Front. Oncol.

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Aberrant metabolism contributes to tumor initiation, progression, metastasis, and drug resistance. Metabolic dysregulation has emerged as a hallmark of several hematologic malignancies. Decoding the molecular mechanism underlying metabolic rewiring in hematological malignancies would provide promising avenues for novel therapeutic interventions. Single-cell metabolic analysis can directly offer a meaningful readout of the cellular phenotype, allowing us to comprehensively dissect cellular states and access biological information unobtainable from bulk analysis. In this review, we first highlight the unique metabolic properties of hematologic malignancies and underscore potential metabolic vulnerabilities. We then emphasize the emerging single-cell metabolomics techniques, aiming to provide a guide to interrogating metabolism at single-cell resolution. Furthermore, we summarize recent studies demonstrating the power of single-cell metabolomics to uncover the roles of metabolic rewiring in tumor biology, cellular heterogeneity, immunometabolism, and therapeutic resistance. Meanwhile, we describe a practical view of the potential applications of single-cell metabolomics in hematopoiesis and hematological malignancies. Finally, we present the challenges and perspectives of single-cell metabolomics development.

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Single-cell technologies and analyses in hematopoiesis and hematological malignancies

Cited by 11 publications

References 153 publications

Clonal hematopoiesis and its impact on the aging osteo-hematopoietic niche

Clonal hematopoiesis and its impact on the aging osteo-hematopoietic niche

Single cell cancer epigenetics

Single-Cell Metabolomics in Hematopoiesis and Hematological Malignancies

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