Single-cell sequencing reveals the new existence form of dermal papilla cells in the hair follicle regeneration of cashmere goats

Yang, Feng; Li, Rui; Zhao, Chen; Che, Tianyu; Guo, Jun‐tao; Xie, Yingxin; Wang, Zhixin; Li, Jinquan; Liu, Zhihong

doi:10.1016/j.ygeno.2022.110316

Cited by 7 publications

(11 citation statements)

References 43 publications

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“…In the third branch, not only were cell connection information transmission and other related functions enriched, but apoptosis-related functions were also enriched in the second gene set. Yang et al [32] found that the second branch of cashmere goat DP cells was enriched in the apoptosis process via a pseudotemporal analysis of cashmere goat DP cells. They speculated that the regulation of normal gene transcription into pseudogenes in the process of apoptosis coordinated hair follicle apoptosis.…”

Section: Discussionmentioning

confidence: 99%

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Interpretation of the Yak Skin Single-Cell Transcriptome Landscape

Zheng,

Ye,

Bao

et al. 2023

Animals

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The morphogenesis of hair follicle structure is accompanied by the differentiation of skin tissue. Mammalian coats are produced by hair follicles. The formation of hair follicles requires signal transmission between the epidermis and dermis. However, knowledge of the transcriptional regulatory mechanism is still lacking. We used single-cell RNA sequencing to obtain 26,573 single cells from the scapular skin of yaks at hair follicle telogen and anagen stages. With the help of known reference marker genes, 11 main cell types were identified. In addition, we further analyzed the DP cell and dermal fibroblast lineages, drew a single-cell map of the DP cell and dermal fibroblast lineages, and elaborated the key genes, signals, and functions involved in cell fate decision making. The results of this study provide a very valuable resource for the analysis of the heterogeneity of DP cells and dermal fibroblasts in the skin and provide a powerful theoretical reference for further exploring the diversity of hair follicle cell types and hair follicle morphogenesis.

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Section: Discussionmentioning

confidence: 99%

“…Cluster 4 expressed the HS cell marker genes IGFBP5 [29] and LHX2 [30]. Cluster 5 expressed the DP cell marker genes LEF1 [31], MSX1 [32], and HOXC13 [32]. Cluster 7 expressed the epidermal cell lineage marker genes KRT5 [27] and KRT15 [33,34].…”

Section: Identification Of Main Cell Types and Analysis Of Characteri...mentioning

confidence: 99%

Interpretation of the Yak Skin Single-Cell Transcriptome Landscape

Zheng,

Ye,

Bao

et al. 2023

Animals

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show abstract

“…The lowest correlation occurred between DP and Mx, revealing striking differences between the two populations whose signaling exchange orchestrates the dynamics of the hair growth. In recent years, with the development of single-cell sequencing technology, the classification of cells related to HF development and the analysis of differential genes have been studied [ 46 – 48 ]. Comparing the results of previous studies with the results of this study, we found the role of some genes in different HF cell populations (Fig.…”

Section: Discussionmentioning

confidence: 99%

Gene network analysis reveals candidate genes related with the hair follicle development in sheep

Zhao

Huang

et al. 2022

BMC Genomics

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Background Merino sheep are the most famous fine wool sheep in the world. They have high wool production and excellent wool quality and have attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibers grown from secondary wool follicles. Therefore, it is necessary to study the development of hair follicles to understand the mechanism of wool production. The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. The hair follicle development process is very complex and poorly understood. The purpose of our research is to identify candidate genes related to hair follicle development, provide a theoretical molecular breeding basis for the cultivation of fine wool sheep, and provide a reference for the problems of hair loss and alopecia areata that affect human beings. Results We analyzed mRNAs data in skin tissues of 18 Merino sheep at four embryonic days (E65, E85, E105 and E135) and two postnatal days (P7 and P30). G1 to G6 represent hair follicles developmental at six stages (i.e. E65 to P30). We identified 7879 differentially expressed genes (DEGs) and 12623 novel DEGs, revealed different expression patterns of these DEGs at six stages of hair follicle development, and demonstrated their complex interactions. DEGs with stage-specific expression were significantly enriched in epidermal differentiation and development, hair follicle development and hair follicle morphogenesis and were enriched in many pathways related to hair follicle development. The key genes (LAMA5, WNT10A, KRT25, SOSTDC1, ZDHHC21, FZD1, BMP7, LRP4, TGFβ2, TMEM79, SOX10, ITGB4, KRT14, ITGA6, and GLI2) affecting hair follicle morphogenesis were identified by network analysis. Conclusion This study provides a new reference for the molecular basis of hair follicle development and lays a foundation for further improving sheep hair follicle breeding. Candidate genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine wool sheep. These results are a valuable resource for biological investigations of fleece evolution in animals.

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“…The DP cells regulate hair follicle development and secondary hair growth [ 18 ], and the quality of villi produced in animals is significantly relational to the secondary hair follicles [ 22 ]. Through scRNA-seq, DP cell lineage differentiation trajectories can be constructed to reveal key genes and functions involved in cell fate determination [ 23 ]. It is expected that in the future, scRNA-seq can comprehensively analyze the fate regulation process of major cell types during animal hair follicle development, provide reliable candidate genes for animal breeding work, improve the yield and quality of animal villi, and find some potential solutions for hair loss.…”

Section: Analysis Of Different Cellsmentioning

confidence: 99%

Understanding Mammalian Hair Follicle Ecosystems by Single-Cell RNA Sequencing

Zheng

Zhang

Bao

et al. 2022

Animals

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Single-cell sequencing technology can fully reflect the heterogeneity of cell populations at the single cell level, making it possible for us to re-recognize various tissues and organs. At present, the sequencing study of hair follicles is transiting from the traditional ordinary transcriptome level to the single cell level, which will provide diverse insights into the function of hair follicle cells. This review focuses on research advances in the hair follicle microenvironment obtained from scRNA-seq studies of major cell types in hair follicle development, with a special emphasis on the discovery of new subpopulations of hair follicles by single-cell techniques. We also discuss the problems and current solutions in scRNA-seq observation and look forward to its prospects.

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Single-cell sequencing reveals the new existence form of dermal papilla cells in the hair follicle regeneration of cashmere goats

Cited by 7 publications

References 43 publications

Interpretation of the Yak Skin Single-Cell Transcriptome Landscape

Interpretation of the Yak Skin Single-Cell Transcriptome Landscape

Gene network analysis reveals candidate genes related with the hair follicle development in sheep

Understanding Mammalian Hair Follicle Ecosystems by Single-Cell RNA Sequencing

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