Single-cell RNA sequencing suggests plasmids constrain bacterial heterogeneity and conjugation is subpopulation specific
Valentine Cyriaque,
Rodrigo Ibarra-Chávez,
Anna Kuchina
et al.
Abstract:Transcriptional heterogeneity is common within isogenic bacterial populations. Nonetheless, the interplay between conjugative plasmids and host subpopulation dynamics remains elusive. Here, the efficacy of microbial split-pool ligation transcriptomics in unraveling plasmid-host interactions was demonstrated through the generation of high-quality single-cell transcriptomic data. We found that transcription was impacted by growth state but also by plasmid carriage at the single-cell level, resulting in a reducti… Show more
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