Single-cell RNA sequencing reveals peripheral blood mononuclear immune cell landscape associated with operational tolerance in a kidney transplant recipient

Azim, Shafquat; Zubair, Haseeb; Rousselle, Thomas; McDaniels, Jennifer; Shetty, Amol; Kuscu, Cem; Kuscu, Canan; Talwar, Manish; Eason, James D.; Maluf, Daniel G.; Mas, Valeria R.

doi:10.1016/j.ajt.2023.04.035

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…Also, studying the kidney behavior at multiple time points after transplantation to understand its dynamic behavior is an important need; however, repeated biopsies are not convenient and safe, if practically possible [124]. Urinary and PBMCs from kidney transplant recipients are other sources of organ injury biomarkers [125]. Transcriptomic analysis and clustering of these cells are considered noninvasive analysis methods on which researchers are working.…”

Section: Kidney Transplantationmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples. This method typically uses microfluidics systems to uncover the complex intercellular communication networks and biological pathways buried within highly heterogeneous cell populations in tissues. One important application of this technology sits in the fields of organ and stem cell transplantation, where complications such as graft rejection and other post-transplantation life-threatening issues may occur. In this review, we first focus on research in which single-cell RNA sequencing is used to study the transcriptional profile of transplanted tissues. This technology enables the analysis of the donor and recipient cells and identifies cell types and states associated with transplant complications and pathologies. We also review the use of single-cell RNA sequencing in stem cell implantation. This method enables studying the heterogeneity of normal and pathological stem cells and the heterogeneity in cell populations. With their remarkably rapid pace, the single-cell RNA sequencing methodologies will potentially result in breakthroughs in clinical transplantation in the coming years.

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Section: Kidney Transplantationmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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Single Cell Sequencing and Spatial Transcriptomics in Kidney Transplantation

Paul,

Atkinson,

Malone

2024

Curr Transpl Rep

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Purpose of Review Progress in biology and medicine relies on technological advances. The ability to examine gene expression from single cells in tissues with complex architecture is now possible with the advent of single cell sequencing and spatial transcriptomics methods. This review will outline how these methods have been applied to kidney transplantation and their inherent challenges and limitations. Recent Findings The transcriptomes of single cells can now be defined in health and disease allowing for the differences to be investigated. Advanced in situ sequencing and capture methodologies now add spatial resolution to single cell transcriptomics. However, such powerful methods bring challenges, most notably the associated computational burden that is required to make the resulting high-dimensional output data interpretable. A second challenge is that of cost. Thankfully, as with many new technologies’ costs will drop over time making these methods available to more research groups. Summary The sequencing and analysis of gene expression at the single cell level with spatial information retained is now feasible. These methods are major advances for biomedical research and bring huge potential with regards to the better understanding of disease at cellular and organ levels.

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Demystifying tolerance

Newell

2023

American Journal of Transplantation

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Single-cell RNA sequencing reveals peripheral blood mononuclear immune cell landscape associated with operational tolerance in a kidney transplant recipient

Cited by 4 publications

References 29 publications

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Single Cell Sequencing and Spatial Transcriptomics in Kidney Transplantation

Demystifying tolerance

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