Single-cell RNA sequencing identifies heterogeneous cell subtypes within gingival tissue

Qian, Shu‐Jiao; Huang, Qing-Guo; Shi, Junyu; Zhou, Liyun; Zhao, Yi; Li, Bin; Lai, Hong-Chang

doi:10.21203/rs.3.rs-101475/v1

Cited by 2 publications

(2 citation statements)

References 60 publications

(64 reference statements)

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“…The rapid advancement of single-cell RNA sequencing (scRNA-seq) and corresponding data analysis algorithms has provided a chance to draw this entire cell interactome. Scientists have applied scRNA-seq to draw the cell atlas of mouth dental development at various stages [16][17][18] and human periodontal tissue [19]. However, the comprehensive cell interactome of human tooth is still lacking.…”

Section: Introductionmentioning

confidence: 99%

A single-cell interactome of human tooth germ from growing third molar elucidates signaling networks regulating dental development

Shi

Zhou

et al. 2021

Cell Biosci

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Background Development of dental tissue is regulated by extensive cell crosstalk based on various signaling molecules, such as bone morphogenetic protein (BMP) and fibroblast growth factor (FGF) pathways. However, an intact network of the intercellular regulation is still lacking. Result To gain an unbiased and comprehensive view of this dental cell interactome, we applied single-cell RNA-seq on immature human tooth germ of the growing third molar, discovered refined cell subtypes, and applied multiple network analysis to identify the central signaling pathways. We found that immune cells made up over 80% of all tooth germ cells, which exhibited profound regulation on dental cells via Transforming growth factor-β, Tumor necrosis factor (TNF) and Interleukin-1. During osteoblast differentiation, expression of genes related to extracellular matrix and mineralization was continuously elevated by signals from BMP and FGF family. As for the self-renewal of apical papilla stem cell, BMP-FGFR1-MSX1 pathway directly regulated the G0-to-S cell cycle transition. We also confirmed that Colony Stimulating Factor 1 secreted from pericyte and TNF Superfamily Member 11 secreted from osteoblast regulated a large proportion of genes related to osteoclast transformation from macrophage and monocyte. Conclusions We constructed the intercellular signaling networks that regulated the essential developmental process of human tooth, which served as a foundation for future dental regeneration engineering and the understanding of oral pathology.

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Section: Introductionmentioning

confidence: 99%

A single-cell interactome of human tooth germ from growing third molar elucidates signaling networks regulating dental development

Shi

Zhou

et al. 2021

Cell Biosci

View full text Add to dashboard Cite

show abstract

“…The rapid advancement of single-cell RNA sequencing (scRNA) and corresponding data analysis algorithms has provided a chance to draw this entire cell interactome. Scientists have applied scRNA to draw the cell atlas of mouth dental development at various stages [16][17][18] and human periodontal tissue [19,20]. However, the comprehensive cell interactome of human tooth is still lacking.…”

Section: Introductionmentioning

confidence: 99%

A Single-cell Interactome of Human Tooth Germ Elucidates Signaling Networks Regulating Dental Development

Shi¹,

Yu²,

Zhang³

et al. 2021

Preprint

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Background: Development of dental tissue is regulated by extensive cell crosstalk based on various signaling molecules, such as BMP, FGF and SHH pathways. However, an intact network of the intercellular regulation is still lacking.Result: To gain an unbiased and comprehensive view of this dental cell interactome, we applied single-cell RNA-seq on immature human tooth germ of the third molar, discovered refined cell subtypes, and applied multiple network analysis to identify the central signaling pathways. We found that immune cells made up over 80% of all tooth germ cells, which exhibited profound regulation on dental cells via TGF-β, TNF and IL-1. During osteoblast differentiation, expression of genes related to extracellular matrix and mineralization was continuously elevated by signals from BMP and FGF family. As for the self-renewal of apical papilla stem cell, BMP-FGFR1-MSX1 pathway directly regulated the G0-to-S cell cycle transition. We also confirmed that CSF1 secreted from pericyte and TNFSF11 secreted from osteoblast regulated a large proportion of genes related to osteoclast transformation from macrophage and monocyte. Conclusion: We constructed the intercellular signaling networks that regulated the essential developmental process of human tooth, which served as a foundation for future dental regeneration engineering and the understanding of oral pathology.

show abstract

Single-cell RNA sequencing identifies heterogeneous cell subtypes within gingival tissue

Cited by 2 publications

References 60 publications

A single-cell interactome of human tooth germ from growing third molar elucidates signaling networks regulating dental development

A single-cell interactome of human tooth germ from growing third molar elucidates signaling networks regulating dental development

A Single-cell Interactome of Human Tooth Germ Elucidates Signaling Networks Regulating Dental Development

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