Single cell RNA-seq reveals cellular diversity and developmental characteristics of human infant retina

Abstract

Retina, located in the innermost layer of the eye of human, holds the decisive role in visual perception. Dissecting the heterogeneity of retina is essential for understanding the mechanism of vision formation and even the development of central nervous system (CNS). Here, we performed single cell RNA-seq, analyzed 57,832 cells from human infant donors, resulting in 20 distinct clusters representing major cell types in retina: rod photoreceptors, cone photoreceptors, bipolar cells, horizontal cells, amacrine cells, Muller glia cells and microglia. We next constructed extensive networks of intercellular communication and identified ligand-receptor interactions playing crucial roles in regulating neural cell development and immune homeostasis in retina. Though re-clustering, we identified known subtypes in cone PRs and additional unreported subpopulations and corresponding markers in rod PRs as well as bipolar cells. Additionally, we linked inherited retinal disease to certain cell subtypes or subpopulations through enrichment analysis. Intriguingly, we found that status and functions of photoreceptors changed drastically between early and late retina. Overall, our study offers the first retinal cell atlas in human infants, dissecting the heterogeneity of retina and identifying the key molecules in the developmental process, which provides an important resource that will pave the way for retina development mechanism research and regenerative medicine concerning retinal biology.

Synthesis, optical properties and application of a set of novel pyrazole nopinone derivatives

“…β-pinene is a main constituent of natural turpentine, and nopinone was directly obtained by oxidating β-pinene with potassium permanganate [13]. Nopinone is easy to develop new derivatives because of its C=O group [14,15], and many nopinone derivatives have been reported [16][17][18][19], such as alkylation derivatives of nopinone [20], chiral annulated indenes [21], nopinone triazole ketones [22], pyridine derivatives of pinene [23], chiral 1,3-aminoalcohols and 1,3-diols [24], 4-pyridyl-2-aminopyrimidine derivatives [25], etc. In addition, some novel nopinone derivatives have been synthesized for a functional application in recent years.…”