Single-cell RNA-Seq revealed profound immune alteration in the peripheral blood of patients with bacterial infection

Lei, Hongxing; Wang, Chi; Wang, Yunlai; Wang, Chengbin

doi:10.1016/j.ijid.2020.11.205

Cited by 16 publications

(15 citation statements)

References 24 publications

(26 reference statements)

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“…Therefore, genes were classified as adaptive or innate based on the immune cells where they are most commonly expressed. For example, T cells (CD4, CD8) (Lei et al, 2021) and B cells (IgG, IgM, IgA, IgE) (Croote et al, 2018) are adaptive immune cells, while NK cells (CD16, NKG2D) (Lei et al, 2021) and macrophages (CD11) are generally classified as innate immune cells (Roitt et al, 2001) (more details in Table 1). This classification does not mean the selected genes are only involved in either adaptive or innate immune responses, most of the chosen genes stimulate multiple immune pathways and can be involved in recruiting additional immune cells.…”

Section: Methodsmentioning

confidence: 99%

Transmissible cancer influences immune gene expression in an endangered marsupial, the Tasmanian devil (Sarcophilus harrisii)

et al. 2022

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Understanding the effects of wildlife diseases on populations requires insight into local environmental conditions, host defence mechanisms, host life‐history trade‐offs, pathogen population dynamics, and their interactions. The survival of Tasmanian devils (Sarcophilus harrisii) is challenged by a novel, fitness limiting pathogen, Tasmanian devil facial tumour disease (DFTD), a clonally transmissible, contagious cancer. In order to understand the devils’ capacity to respond to DFTD, it is crucial to gain information on factors influencing the devils’ immune system. By using RT‐qPCR, we investigated how DFTD infection in association with intrinsic (sex and age) and environmental (season) factors influences the expression of 10 immune genes in Tasmanian devil blood. Our study showed that the expression of immune genes (both innate and adaptive) differed across seasons, a pattern that was altered when infected with DFTD. The expression of immunogbulins IgE and IgM:IgG showed downregulation in colder months in DFTD infected animals. We also observed strong positive association between the expression of an innate immune gene, CD16, and DFTD infection. Our results demonstrate that sampling across seasons, age groups and environmental conditions are beneficial when deciphering the complex ecoevolutionary interactions of not only conventional host‐parasite systems, but also of host and diseases with high mortality rates, such as transmissible cancers.

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Section: Methodsmentioning

confidence: 99%

Transmissible cancer influences immune gene expression in an endangered marsupial, the Tasmanian devil (Sarcophilus harrisii)

et al. 2022

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“…We noted that the proportion of cells with divergent gene and hashtag antibody UMIs correlated directly with the expected proportion of granulocytes in these samples. Notably, while mouse granulocytes have been successfully characterized by scRNA-seq using the 10X Genomics platform [9][10][11] , granulocytes are largely absent or extremely under-represented in human data scRNA-seq datasets generated using the 10X Genomics platform 12,13 . Despite their low gene expression, we reasoned that the robust detection of antibody-derived UMIs would allow for accurate identification and deeper phenotypic characterization of these cell populations using CITE-seq.…”

Section: Cite-seq Analyses With the 10x Genomics Platform Allow For Accurate Enumeration And Antibodybased Characterization Of Human Granmentioning

confidence: 99%

Multimodal Single-Cell Characterization of the Human Granulocyte Lineage

D’souza

Dawson

et al. 2021

Preprint

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High throughput single cell transcriptomics (scRNA-seq) has been successfully applied to characterize immune cell heterogeneity across a diverse range of settings; however, analysis of human granulocytes remains a significant challenge due to their low gene expression transcript detection. Consequently, granulocytes are typically either absent or highly under-represented and inaccurately enumerated in most human scRNA-seq datasets. Here, we apply multi-modal CITE-seq profiling to characterize granulocytes in human whole blood and bone marrow, and we show that these populations can be accurately detected and analyzed using the antibody-based modality, and that their frequencies and phenotype align well with antibody-based characterization of the same samples using CyTOF. These analyses also clearly highlight extremely low gene transcript detection across the entire granulocyte lineage including the earliest neutrophil progenitor populations when using the 10X Genomics platform. By contrast, when performing parallel analyses of the same samples using the BD Rhapsody platform, we recovered a much higher proportion of granulocyte gene transcripts, enabling true multi-modal characterization of human granulocyte heterogeneity.

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“…Among them, Staphylococcus aureus (S. aureus) is a common zoonotic pathogen that can cause toxin-mediated symptoms and breast infections and is commonly responsible for mastitis. [1][2][3][4] The development of antibacterial materials has received great attention from scientists, and research on new antibacterial materials plays an important role in the development of methods to treat bacterial infections. 5,6 Au nanorods (Au NRs) have outstanding biocompatibility and unique optical properties, 7 including horizontal and vertical surface plasmon resonance (SPR) double-spectral peaks.…”

Section: Introductionmentioning

confidence: 99%

Dual functions of epigallocatechin gallate surface-modified Au nanorods@selenium composites for near-infrared-II light-responsive synergistic antibacterial therapy

Shao

Zhao

et al. 2022

J Biomater Appl

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Diseases caused by bacterial infections pose ever-increasing threats to human health, making it important to explore alternative antibacterial strategies. Herein, epigallocatechin gallate (EGCG) surface-modified Au nanorods@selenium composites (ASE NPs) were developed for synergistic NIR-II light-responsive antibacterial therapy. In vitro antibacterial experiments demonstrated the improved antibacterial effect of ASE NPs against Staphylococcus aureus ( S. aureus) compared with EGCG alone. In addition, in vivo studies demonstrated that ASE NPs cured skin wound infections and sepsis in mice caused by S. aureus. Au nanorods with excellent photothermal conversion realized synergistic photothermal therapy (PTT) in the NIR-II biowindow with an improved penetration depth at a low power density. More importantly, toxicity analysis showed that the composites had no toxic effects on major organs. Thus, the EGCG surface-modified Au nanorods@selenium composites with an NIR-II light-responsive synergistic activity hold great promise for the effective treatment of drug-resistant bacterial infections.

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Single-cell RNA-Seq revealed profound immune alteration in the peripheral blood of patients with bacterial infection

Cited by 16 publications

References 24 publications

Transmissible cancer influences immune gene expression in an endangered marsupial, the Tasmanian devil (Sarcophilus harrisii)

Transmissible cancer influences immune gene expression in an endangered marsupial, the Tasmanian devil (Sarcophilus harrisii)

Multimodal Single-Cell Characterization of the Human Granulocyte Lineage

Dual functions of epigallocatechin gallate surface-modified Au nanorods@selenium composites for near-infrared-II light-responsive synergistic antibacterial therapy

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