Single-Cell Protein from C1 Compounds

Krug, Elizabeth L.R.; Lim, Henry C.; Tsao, George T.

doi:10.1016/b978-0-12-040303-5.50012-5

Cited by 9 publications

(1 citation statement)

References 134 publications

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“…This effect was eliminated when optimum levels of nitrogen and phosphorus were used. Krug et al (1979) reported that magnesium is an essential element of the key enzyme hexulose phosphate synthetase of microorganisms assimilating methanol by the ribulose phosphate pathway. This may explain the high Mg +÷ requirement (24-50 ppm) of the mixed bacterial culture.…”

Section: Discussionmentioning

confidence: 99%

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

Aburuwaida

Banat

Hamdan

1990

Appl Microbiol Biotechnol

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A continuous culture technique was used to optimize the medium composition and growth conditions of a mixed bacterial culture utilizing methanol. The improved medium resuited in satisfactory growth, high-yield coefficients and gave a product containing reduced polysaccharide concentrations. Optimal growth and biomass yields occurred at pH 6.8 a temperature of 37°C and dissolved oxygen at >20% saturation. The maximum growth rate was 0.58 h -1 and maximum biomass yield 0.48 g g-1. The protein content of the product ranged between 81%-83%, and nucleic acid content between 10%-12%, increasing with growth rate. The amino acid profile of the mixed culture product m e t and, in some cases, exceeded the UN Food and Agricultural Organization standard, indicating a good source of feed protein.

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Section: Discussionmentioning

confidence: 99%

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

Aburuwaida

Banat

Hamdan

1990

Appl Microbiol Biotechnol

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An operational strategy for unstable recombinant DNA cultures

Ryder

DiBiasio

1984

Biotech & Bioengineering

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A simple operational strategy is shown to offer a viable means of enhancing plasmid stability in chemostat systems where plasmid loss is a common problem. Feedback control can be used to stabilize coexistence states, which are naturally unstable in the system investigated, and thus gurantee retention of the plasmid-carrying strain. The strategy exploits the normally undesirable characteristics of substrate inhibited growth kinetics, and is illustrated with specific reference to methanolutilizing organisms. Since the methodology may be easily implemented in practice, it offers an alternative to costly environmental methods such as antibiotic addition.

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Direct measurement of carbon substrate oxidation and incorporation patterns in RuMP‐type methylotrophs: Chemostatic cultures of Methylomonas L3

Chu

Bussineau

Papoutsakis

1985

Biotech & Bioengineering

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A technique using C-14 isotope tracers to probe the branching of carbon flow in methylotrophic bacteria has been devised and applied to continuous steady-state cultures. Methylomonas L3, a strain which utilizes the KDPG/TA variant of the ribulose monophosphate cycle for carbon fixation, was employed in the experimental studies. The actual in vivo rates of substrate-carbon incorporation into biomass, both direct and via CO(2), and of the two carbon oxidation schemes were determined in three different steady-state cultures. The results show that the carbon substrate is oxidized predominantly via formate (the linear oxidation scheme), and that the cyclic scheme of oxidation is minimally, if at all, utilized. The carbon incorporation and oxidation patterns appear to vary considerably with the dilution rate and the inoculum history. The experimental accuracy of the new technique is discussed in detail.

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Single-Cell Protein from C1 Compounds

Cited by 9 publications

References 134 publications

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

An operational strategy for unstable recombinant DNA cultures

Direct measurement of carbon substrate oxidation and incorporation patterns in RuMP‐type methylotrophs: Chemostatic cultures of Methylomonas L3

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