Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

Hou, Helei; Zhao, Yangyang; Li, Chuanping; Wang, Minmin; Xu, Xiaolong; Jin, Yongdong

doi:10.1038/s41598-017-01956-1

Cited by 65 publications

(73 citation statements)

References 30 publications

(41 reference statements)

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“…The small impact of HAp nanoobjects on A549 cells is probably connected to their cancerous nature and lower intracellular pH value [77]. Even after a high uptake of HAp particles, A549 could dissolve hydroxyapatite more efficiently and safely than the other studied cells.…”

Section: Resultsmentioning

confidence: 99%

Characterization and influence of hydroxyapatite nanopowders on living cells

Oberbek

Bolek

Chlanda

et al. 2018

Beilstein J. Nanotechnol.

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Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and respiratory systems.

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Section: Resultsmentioning

confidence: 99%

Characterization and influence of hydroxyapatite nanopowders on living cells

Oberbek

Bolek

Chlanda

et al. 2018

Beilstein J. Nanotechnol.

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“…Novel vaccines with higher clinical efficacy can be developed using results from antibody screening and quantification experiments at single-cell level ( 149 , 166 ). Also, quantification of signaling molecules at single-cell level provides information on new pathways for development of sensitive diagnostic tools that can provide faster and accurate results in comparison to traditional laboratory methods ( 189 ).…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Integrating Immunology and Microfluidics for Single Immune Cell Analysis

2018

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The field of immunoengineering aims to develop novel therapies and modern vaccines to manipulate and modulate the immune system and applies innovative technologies toward improved understanding of the immune system in health and disease. Microfluidics has proven to be an excellent technology for analytics in biology and chemistry. From simple microsystem chips to complex microfluidic designs, these platforms have witnessed an immense growth over the last decades with frequent emergence of new designs. Microfluidics provides a highly robust and precise tool which led to its widespread application in single-cell analysis of immune cells. Single-cell analysis allows scientists to account for the heterogeneous behavior of immune cells which often gets overshadowed when conventional bulk study methods are used. Application of single-cell analysis using microfluidics has facilitated the identification of several novel functional immune cell subsets, quantification of signaling molecules, and understanding of cellular communication and signaling pathways. Single-cell analysis research in combination with microfluidics has paved the way for the development of novel therapies, point-of-care diagnostics, and even more complex microfluidic platforms that aid in creating in vitro cellular microenvironments for applications in drug and toxicity screening. In this review, we provide a comprehensive overview on the integration of microsystems and microfluidics with immunology and focus on different designs developed to decode single immune cell behavior and cellular communication. We have categorized the microfluidic designs in three specific categories: microfluidic chips with cell traps, valve-based microfluidics, and droplet microfluidics that have facilitated the ongoing research in the field of immunology at single-cell level.

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“…The development of reversible reaction-based fluorescent probe, such as RealThiol (RT) [ 163 ], that can quantitatively monitor real-time glutathione dynamics in living cells may also find use in delineating this biomarker in vivo. Additionally, both Hu et al [ 164 ] and Wang et al [ 165 ] recently described techniques which allow accurate and sensitive pH detection on the single-cell or sub-cell level indicating the ability to assess PT-related biomarkers in great detail. Hu et al [ 164 ] employed an innovative combination of UV-Vis microspectroscopy and common pH indicators, while Wang et al [ 165 ] applied ultra pH-sensitive fluorescent nanoprobes that display tunable, exponential fluorescence activation on encountering subtle, physiologically relevant pH transitions.…”

Section: Hurdle 3: Intracellular Release Of Active Agentmentioning

confidence: 99%

Polymer Therapeutics: Biomarkers and New Approaches for Personalized Cancer Treatment

Atkinson

Andreu

Vicent

2018

JPM

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Polymer therapeutics (PTs) provides a potentially exciting approach for the treatment of many diseases by enhancing aqueous solubility and altering drug pharmacokinetics at both the whole organism and subcellular level leading to improved therapeutic outcomes. However, the failure of many polymer-drug conjugates in clinical trials suggests that we may need to stratify patients in order to match each patient to the right PT. In this concise review, we hope to assess potential PT-specific biomarkers for cancer treatment, with a focus on new studies, detection methods, new models and the opportunities this knowledge will bring for the development of novel PT-based anti-cancer strategies. We discuss the various “hurdles” that a given PT faces on its passage from the syringe to the tumor (and beyond), including the passage through the bloodstream, tumor targeting, tumor uptake and the intracellular release of the active agent. However, we also discuss other relevant concepts and new considerations in the field, which we hope will provide new insight into the possible applications of PT-related biomarkers.

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Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

Cited by 65 publications

References 30 publications

Characterization and influence of hydroxyapatite nanopowders on living cells

Characterization and influence of hydroxyapatite nanopowders on living cells

Integrating Immunology and Microfluidics for Single Immune Cell Analysis

Polymer Therapeutics: Biomarkers and New Approaches for Personalized Cancer Treatment

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