Single Cell Oil Production from Undetoxified Arundo donax  L. hydrolysate by Cutaneotrichosporon curvatus

Fidio, Nicola Di; Liuzzi, Federico; Mastrolitti, Silvio; Albergo, Roberto; Bari, Isabella De

doi:10.4014/jmb.1808.08015

Cited by 30 publications

(28 citation statements)

References 42 publications

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“…This is certainly a positive key aspect, enabling the direct biological conversion of these hydrolysates, without additional further relevant detoxification procedures [46,47]. In fact, in the fermentative route for sugars valorisation, the by-products act as strong inhibitors for microorganisms [55]. Therefore, the synthesized hydrolysate, characterized by a very low amount of by-products and a high quantity of reducing sugars (46.1 g/L), represents an ideal substrate for the production of value-added bio-chemicals through a subsequent biological route.…”

Section: Microwave-assisted Hydrolysis Of Giant Reed Hemicellulose Tomentioning

confidence: 99%

“…For this reason, Arundo donax L. is widely used as a substrate for the synthesis of important platform-chemicals, biofuels, and second-generation sugars [14,16,25,27,35,[46][47][48]. In particular, the interest towards the synthesis of xylose and glucose is continuously growing, due to their promising applications in both chemical and biological processes to produce alcohols, acids, oils, hydrocarbons, hydrogen, and other valuable products [1,15,22,34,46,47,[49][50][51][52][53][54][55]. However, up to now, only a few works discussed the employment of acid solid catalysts for the conversion of Arundo donax L. to value-added products, performing the reaction in the presence of ionic liquids as solvents, which have some criticisms related to their high viscosity, toxicity and cost, which strongly limit the sustainability of this approach.…”

Section: Introductionmentioning

confidence: 99%

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Multi-Step Exploitation of Raw Arundo donax L. for the Selective Synthesis of Second-Generation Sugars by Chemical and Biological Route

et al. 2020

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Lignocellulosic biomass represents one of the most important feedstocks for future biorefineries, being a precursor of valuable bio-products, obtainable through both chemical and biological conversion routes. Lignocellulosic biomass has a complex matrix, which requires the careful development of multi-step approaches for its complete exploitation to value-added compounds. Based on this perspective, the present work focuses on the valorization of hemicellulose and cellulose fractionsof giant reed (Arundo donax L.) to give second-generation sugars, minimizing the formation of reaction by-products. The conversion of hemicellulose to xylose was undertaken in the presence of the heterogeneous acid catalyst Amberlyst-70 under microwave irradiation. The effect of the main reaction parameters, such as temperature, reaction time, catalyst, and biomass loadings on sugars yield was studied, developing a high gravity approach. Under the optimised reaction conditions (17 wt% Arundo donax L. loading, 160 °C, Amberlyst-70/Arundo donax L. weight ratio 0.2 wt/wt), the xylose yield was 96.3 mol%. In the second step, the cellulose-rich solid residue was exploited through the chemical or enzymatic route, obtaining glucose yields of 32.5 and 56.2 mol%, respectively. This work proves the efficiency of this innovative combination of chemical and biological catalytic approaches, for the selective conversion of hemicellulose and cellulose fractions of Arundo donax L. to versatile platform products.

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Section: Microwave-assisted Hydrolysis Of Giant Reed Hemicellulose Tomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multi-Step Exploitation of Raw Arundo donax L. for the Selective Synthesis of Second-Generation Sugars by Chemical and Biological Route

et al. 2020

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“…Oleaginous microorganisms are characterised by the ability to accumulate high concentrations of lipids into their cells as energy storage. Several studies demonstrated the potential use of SCO as a good alternative to vegetable oil to produce new-generation biodiesels and other bioproducts [3,7,17,18], such as biosurfactants, biopolymers, cosmetics and animal feed (e.g., fish cultures) [14,19,20].…”

Section: Introductionmentioning

confidence: 99%

“…In fact, some organic compounds, such as furaldehyde, formaldehyde, phenols, aliphatic acids, vanillic acid, uronic acid, 4-hydroxybenzoic acid, acetic acid and cinnamaldehyde, can act as inhibitors for different metabolic activities of oleaginous yeasts [27,28]. The susceptibility of microorganisms to inhibitors strictly depends on the strain they belong to, so it is necessary to adapt the fermentation process to the type of microorganism used, in order to increase the cell biomass and lipid production [4,7,17].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, C. oleaginosus is fast growing, is able to use a wide range of carbon sources, can accumulate a high content of TAGs (up to 60% of its dry cell weight) [27] and presents good tolerance to the main growth inhibitors. In particular, this yeast can grow on carbon-rich media obtained from lignocellulosic biomasses [17], organic acids [31], organic wastes from the agriculture and food industry [32] as well as active sludge [33]. Moreover, the lipid profile is characterised by over 50% of unsaturated long-chain fatty acids, with a high quantity of oleic acid and linolenic acid.…”

Section: Introductionmentioning

confidence: 99%

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Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

et al. 2021

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Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It is fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it a promising biocatalyst for custom tailored microbial oils. C. oleaginosus can accumulate up to 60 wt.% of its biomass as lipids. This species is able to grow by using several compounds as a substrate, such as acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper and other agro-industrial wastes. This review is focused on state-of-the-art innovative and sustainable biorefinery schemes involving this promising yeast and second- and third-generation biomasses. Moreover, this review offers a comprehensive and updated summary of process strategies, biomass pretreatments and fermentation conditions for enhancing lipid production by C. oleaginosus as a whole-cell biocatalyst. Finally, an overview of the main industrial applications of single-cell oil is reported together with future perspectives.

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Sustainable Valorisation and Efficient Downstream Processing of Giant Reed by High‐Pressure Carbon Dioxide Pretreatment

et al. 2022

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This work investigated the catalytic high‐pressure CO2 pretreatment of giant reed. CO2 is a renewable resource; its use does not generate chemical wastes and it can be easily removed and recycled. The effect of the addition of low concentrations of FeCl3 (0.16 wt %) and PEG 400 (1.0 wt %) on the hemicellulose hydrolysis to xylose and xylo‐oligosaccharides (XOS) is reported for the first time. Under the optimised pretreatment conditions, the xylan conversion of 82 mol % and xylose and XOS yields of 43 and 20 mol % were achieved, respectively. The solid residues obtained from different pretreatments were used as the substrate for the enzymatic hydrolysis to give glucose. The total glucose yield achieved under the optimised two‐step process was 67.8 mol % with respect to the glucan units in the biomass. The results demonstrated that PEG‐assisted FeCl3‐catalysed scCO2 pretreatment can produce xylose‐ or XOS‐rich hydrolysates and improve the enzymatic hydrolysis of biomass.

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Single Cell Oil Production from Undetoxified Arundo donax L. hydrolysate by Cutaneotrichosporon curvatus

Cited by 30 publications

References 42 publications

Multi-Step Exploitation of Raw Arundo donax L. for the Selective Synthesis of Second-Generation Sugars by Chemical and Biological Route

Multi-Step Exploitation of Raw Arundo donax L. for the Selective Synthesis of Second-Generation Sugars by Chemical and Biological Route

Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Sustainable Valorisation and Efficient Downstream Processing of Giant Reed by High‐Pressure Carbon Dioxide Pretreatment

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