Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles

Kim, Ji Wook; Seo, Daeha; Lee, Jung Uk; Southard, Kaden M.; Lim, Yongjun; Kim, Dae Hyun; Gartner, Zev J.; Jun, Young‐wook; Cheon, Jinwoo

doi:10.1038/nprot.2017.071

Cited by 48 publications

(67 citation statements)

References 68 publications

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“…For the current experiments, zinc-doped iron oxide magnetic nanoparticles (MNPs) were synthesized following the previous reports [33,35,36]. In brief, Fe(acac) 3 (353 mg), FeCl 2 (40 mg) and ZnCl 2 (30 mg) were dissolved in octyl ether with organic surfactants (oleic acid and oleylamine).…”

Section: Mnp Synthesismentioning

confidence: 99%

Thermoresponsive Behavior of Magnetic Nanoparticle Complexed pNIPAm-co-AAc Microgels

2018

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Characterization of responsive hydrogels and their enhancement with novel moieties have improved our understanding of functional materials. Hydrogels coupled with inorganic nanoparticles have been sought for novel types of responsive materials, but the efficient routes for the formation and the responsivity of complexed materials remain for further investigation. Here, we report that responsive poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) hydrogel microparticles (microgels) are tunable by varying composition of co-monomer and crosslinker as well as by their complexation with magnetic nanoparticles in aqueous dispersions. Our results show that the hydrodynamic diameter and thermoresponsivity of microgels are closely related with the composition of anionic co-monomer, AAc and crosslinker, N,N′-Methylenebisacrylamide (BIS). As a composition of hydrogels, the higher AAc increases the swelling size of the microgels and the volume phase transition temperature (VPTT), but the higher BIS decreases the size with no apparent effect on the VPTT. When the anionic microgels are complexed with amine-modified magnetic nanoparticles (aMNP) via electrostatic interaction, the microgels decrease in diameter at 25 °C and shift the volume phase transition temperature (VPTT) to a higher temperature. Hysteresis on the thermoresponsive behavior of microgels is also measured to validate the utility of aMNP-microgel complexation. These results suggest a simple, yet valuable route for development of advanced responsive microgels, which hints at the formation of soft nanomaterials enhanced by inorganic nanoparticles.

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Section: Mnp Synthesismentioning

confidence: 99%

Thermoresponsive Behavior of Magnetic Nanoparticle Complexed pNIPAm-co-AAc Microgels

2018

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“…Nowadays, with the development of science and technology, the applications of magnetic materials have been extended to biology and medicine . In particular, magnetic manipulation of cells using forces induced via magnetic nanomaterials (MNs) is becoming an attractive research area in mechanotransduction with important biomedical implications …”

Section: Introductionmentioning

confidence: 99%

“…MN‐based mechanotransduction has become an important approach to noninvasively and precisely control the forces applied to cells. For example, specific‐biomolecule‐tethered MNs can generate mechanical forces and/or torques under the control of a magnetic field setup to the associated or attached cells …”

Section: Introductionmentioning

confidence: 99%

“…For instance, a single magnetic force pulse with 0.29 ± 0.06 pN was able to open the mechanoelectrical transduction channels in hair cells . This falls into the right range of forces that MNs can generate on the attached proteins, ion channels, or lipids . Therefore, specific MNs have been developed as mechanotransduction tools to modulate cell fate .…”

Section: Introductionmentioning

confidence: 99%

“…This falls into the right range of forces that MNs can generate on the attached proteins, ion channels, or lipids . Therefore, specific MNs have been developed as mechanotransduction tools to modulate cell fate . The design of these MNs offers maneuverability under an external magnetic field through their fine‐tuned compositions and geometries to generate localized forces on cells .…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Magnetic‐Nanomaterial‐Based Mechanotransduction for Cell Fate Regulation

Shen

Chen

et al. 2018

Advanced Materials

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Remote control of cells and the regulation of cell events at the molecular level are of great interest in the biomedical field. In addition to chemical compounds and genes, mechanical forces play a pivotal role in regulating cell fate, which have prompted the rapid growth of mechanobiology. From a perspective of nanotechnology, magnetic nanomaterials (MNs) are an appealing option for mechanotransduction due to their capabilities in spatiotemporal manipulation of mechanical forces via the magnetic field. As a newly developed paradigm, magneto-mechanotransduction is harnessed to physically regulate cell fate for biomedical applications. Here, the critical factors that determine the magnetomechanical forces induced by MNs in mechanotransduction are briefly reviewed. Recent innovative approaches and their underlying mechanisms for controlling cell fate are highlighted, which offer possibilities for the remote mechanical manipulation of cells and biomolecules in a precise manner. Promising applications including regenerative medicine and cancer treatment based on magnetomechanical stimulation through MNs are also addressed. Perspectives and challenges in MN-based mechanotransduction are commented.

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Immunoregulation of Macrophages by Controlling Winding and Unwinding of Nanohelical Ligands

Bae

Jeon

et al. 2021

Adv Funct Materials

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Developing materials with the capability of changing their innate features can help to unravel direct interactions between cells and ligand‐displaying features. This study demonstrates the grafting of magnetic nanohelices displaying cell‐adhesive Arg‐Gly‐Asp (RGD) ligand partly to a material surface. These enable nanoscale control of rapid winding (“W”) and unwinding (“UW”) of their nongrafted portion, such as directional changes in nanohelix unwinding (lower, middle, and upper directions) by changing the position of a permanent magnet while keeping the ligand‐conjugated nanohelix surface area constant. The unwinding (“UW”) setting cytocompatibility facilitates direct integrin recruitment onto the ligand‐conjugated nanohelix to mediate the development of paxillin adhesion assemblies of macrophages that stimulate M2 polarization using glass and silicon substrates for in vitro and in vivo settings, respectively, at a single cell level. Real time and in vivo imaging are demonstrated that nanohelices exhibit reversible unwinding, winding, and unwinding settings, which modulate time‐resolved adhesion and polarization of macrophages. It is envisaged that this remote, reversible, and cytocompatible control can help to elucidate molecular‐level cell–material interactions that modulate regenerative/anti‐inflammatory immune responses to implants.

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Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles

Cited by 48 publications

References 68 publications

Thermoresponsive Behavior of Magnetic Nanoparticle Complexed pNIPAm-co-AAc Microgels

Thermoresponsive Behavior of Magnetic Nanoparticle Complexed pNIPAm-co-AAc Microgels

Recent Advances in Magnetic‐Nanomaterial‐Based Mechanotransduction for Cell Fate Regulation

Immunoregulation of Macrophages by Controlling Winding and Unwinding of Nanohelical Ligands

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